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Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)

Late embryogenesis-abundant (LEA) genes play important roles in plant growth and development, especially the cellular dehydration tolerance during seed maturation. In order to comprehensively understand the roles of LEA family members in wheat, we carried out a series of analyses based on the latest...

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Autores principales: Liu, Datong, Sun, Jing, Zhu, Dongmei, Lyu, Guofeng, Zhang, Chunmei, Liu, Jian, Wang, Hui, Zhang, Xiao, Gao, Derong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770980/
https://www.ncbi.nlm.nih.gov/pubmed/31510067
http://dx.doi.org/10.3390/genes10090696
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author Liu, Datong
Sun, Jing
Zhu, Dongmei
Lyu, Guofeng
Zhang, Chunmei
Liu, Jian
Wang, Hui
Zhang, Xiao
Gao, Derong
author_facet Liu, Datong
Sun, Jing
Zhu, Dongmei
Lyu, Guofeng
Zhang, Chunmei
Liu, Jian
Wang, Hui
Zhang, Xiao
Gao, Derong
author_sort Liu, Datong
collection PubMed
description Late embryogenesis-abundant (LEA) genes play important roles in plant growth and development, especially the cellular dehydration tolerance during seed maturation. In order to comprehensively understand the roles of LEA family members in wheat, we carried out a series of analyses based on the latest genome sequence of the bread wheat Chinese Spring. 121 Triticum aestivum L. LEA (TaLEA) genes, classified as 8 groups, were identified and characterized. TaLEA genes are distributed in all chromosomes, most of them with a low number of introns (≤3). Expression profiles showed that most TaLEA genes expressed specifically in grains. By qRT-PCR analysis, we confirmed that 12 genes among them showed high expression levels during late stage grain maturation in two spring wheat cultivars, Yangmai16 and Yangmai15. For most genes, the peak of expression appeared earlier in Yangmai16. Statistical analysis indicated that expression level of 8 genes in Yangmai 16 were significantly higher than Yangmai 15 at 25 days after anthesis. Taken together, our results provide more knowledge for future functional analysis and potential utilization of TaLEA genes in wheat breeding.
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spelling pubmed-67709802019-10-30 Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.) Liu, Datong Sun, Jing Zhu, Dongmei Lyu, Guofeng Zhang, Chunmei Liu, Jian Wang, Hui Zhang, Xiao Gao, Derong Genes (Basel) Article Late embryogenesis-abundant (LEA) genes play important roles in plant growth and development, especially the cellular dehydration tolerance during seed maturation. In order to comprehensively understand the roles of LEA family members in wheat, we carried out a series of analyses based on the latest genome sequence of the bread wheat Chinese Spring. 121 Triticum aestivum L. LEA (TaLEA) genes, classified as 8 groups, were identified and characterized. TaLEA genes are distributed in all chromosomes, most of them with a low number of introns (≤3). Expression profiles showed that most TaLEA genes expressed specifically in grains. By qRT-PCR analysis, we confirmed that 12 genes among them showed high expression levels during late stage grain maturation in two spring wheat cultivars, Yangmai16 and Yangmai15. For most genes, the peak of expression appeared earlier in Yangmai16. Statistical analysis indicated that expression level of 8 genes in Yangmai 16 were significantly higher than Yangmai 15 at 25 days after anthesis. Taken together, our results provide more knowledge for future functional analysis and potential utilization of TaLEA genes in wheat breeding. MDPI 2019-09-10 /pmc/articles/PMC6770980/ /pubmed/31510067 http://dx.doi.org/10.3390/genes10090696 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Datong
Sun, Jing
Zhu, Dongmei
Lyu, Guofeng
Zhang, Chunmei
Liu, Jian
Wang, Hui
Zhang, Xiao
Gao, Derong
Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)
title Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)
title_full Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)
title_fullStr Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)
title_full_unstemmed Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)
title_short Genome-Wide Identification and Expression Profiles of Late Embryogenesis-Abundant (LEA) Genes during Grain Maturation in Wheat (Triticum aestivum L.)
title_sort genome-wide identification and expression profiles of late embryogenesis-abundant (lea) genes during grain maturation in wheat (triticum aestivum l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6770980/
https://www.ncbi.nlm.nih.gov/pubmed/31510067
http://dx.doi.org/10.3390/genes10090696
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