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The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
BACKGROUND: The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771094/ https://www.ncbi.nlm.nih.gov/pubmed/31570097 http://dx.doi.org/10.1186/s12867-019-0140-0 |
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author | Li, Fan Wang, Yun Yang, Hui Xu, Yingying Zhou, Xiaoyan Zhang, Xiao Xie, Zhaohong Bi, Jianzhong |
author_facet | Li, Fan Wang, Yun Yang, Hui Xu, Yingying Zhou, Xiaoyan Zhang, Xiao Xie, Zhaohong Bi, Jianzhong |
author_sort | Li, Fan |
collection | PubMed |
description | BACKGROUND: The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1 mRNA and promotes the formation of Aβ. After the administration of Aβ(1-42) to SH-SY5Y cells and C57/BL6J mice, we detected the expression of BACE1-AS, BACE1 mRNA, and BACE1 protein, as well as the concentration of Aβ(1-40). Then, we silenced the expression of BACE1-AS in SH-SY5Y and 20E2 cells using siRNAs targeting BACE1-AS and detected its effects on the levels of the BACE1 mRNA and BACE1 protein and Aβ(1-40) generation. RESULTS: The administration of Aβ(1-42) increased the expression of BACE1-AS, BACE1 mRNA and protein, as well as the concentration of Aβ(1-40) in SH-SY5Y cells and the brains of C57BL/6J mice. Pretreatment with the BACE1-AS siRNA inhibited the effect of Aβ(1-42) on increasing the expression of BACE1-AS and BACE1, as well as the generation of Aβ. CONCLUSIONS: The mechanism by which exogenous Aβ(1-42) induces BACE1 expression and Aβ generation is mediated by BACE1-AS. BACE1-AS is involved in the mechanism regulating BACE1 expression and Aβ generation in APPsw transgenic cells. |
format | Online Article Text |
id | pubmed-6771094 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-67710942019-10-03 The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression Li, Fan Wang, Yun Yang, Hui Xu, Yingying Zhou, Xiaoyan Zhang, Xiao Xie, Zhaohong Bi, Jianzhong BMC Mol Biol Research Article BACKGROUND: The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1 mRNA and promotes the formation of Aβ. After the administration of Aβ(1-42) to SH-SY5Y cells and C57/BL6J mice, we detected the expression of BACE1-AS, BACE1 mRNA, and BACE1 protein, as well as the concentration of Aβ(1-40). Then, we silenced the expression of BACE1-AS in SH-SY5Y and 20E2 cells using siRNAs targeting BACE1-AS and detected its effects on the levels of the BACE1 mRNA and BACE1 protein and Aβ(1-40) generation. RESULTS: The administration of Aβ(1-42) increased the expression of BACE1-AS, BACE1 mRNA and protein, as well as the concentration of Aβ(1-40) in SH-SY5Y cells and the brains of C57BL/6J mice. Pretreatment with the BACE1-AS siRNA inhibited the effect of Aβ(1-42) on increasing the expression of BACE1-AS and BACE1, as well as the generation of Aβ. CONCLUSIONS: The mechanism by which exogenous Aβ(1-42) induces BACE1 expression and Aβ generation is mediated by BACE1-AS. BACE1-AS is involved in the mechanism regulating BACE1 expression and Aβ generation in APPsw transgenic cells. BioMed Central 2019-10-01 /pmc/articles/PMC6771094/ /pubmed/31570097 http://dx.doi.org/10.1186/s12867-019-0140-0 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Li, Fan Wang, Yun Yang, Hui Xu, Yingying Zhou, Xiaoyan Zhang, Xiao Xie, Zhaohong Bi, Jianzhong The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression |
title | The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression |
title_full | The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression |
title_fullStr | The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression |
title_full_unstemmed | The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression |
title_short | The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression |
title_sort | effect of bace1-as on β-amyloid generation by regulating bace1 mrna expression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771094/ https://www.ncbi.nlm.nih.gov/pubmed/31570097 http://dx.doi.org/10.1186/s12867-019-0140-0 |
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