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The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression

BACKGROUND: The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1...

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Autores principales: Li, Fan, Wang, Yun, Yang, Hui, Xu, Yingying, Zhou, Xiaoyan, Zhang, Xiao, Xie, Zhaohong, Bi, Jianzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771094/
https://www.ncbi.nlm.nih.gov/pubmed/31570097
http://dx.doi.org/10.1186/s12867-019-0140-0
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author Li, Fan
Wang, Yun
Yang, Hui
Xu, Yingying
Zhou, Xiaoyan
Zhang, Xiao
Xie, Zhaohong
Bi, Jianzhong
author_facet Li, Fan
Wang, Yun
Yang, Hui
Xu, Yingying
Zhou, Xiaoyan
Zhang, Xiao
Xie, Zhaohong
Bi, Jianzhong
author_sort Li, Fan
collection PubMed
description BACKGROUND: The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1 mRNA and promotes the formation of Aβ. After the administration of Aβ(1-42) to SH-SY5Y cells and C57/BL6J mice, we detected the expression of BACE1-AS, BACE1 mRNA, and BACE1 protein, as well as the concentration of Aβ(1-40). Then, we silenced the expression of BACE1-AS in SH-SY5Y and 20E2 cells using siRNAs targeting BACE1-AS and detected its effects on the levels of the BACE1 mRNA and BACE1 protein and Aβ(1-40) generation. RESULTS: The administration of Aβ(1-42) increased the expression of BACE1-AS, BACE1 mRNA and protein, as well as the concentration of Aβ(1-40) in SH-SY5Y cells and the brains of C57BL/6J mice. Pretreatment with the BACE1-AS siRNA inhibited the effect of Aβ(1-42) on increasing the expression of BACE1-AS and BACE1, as well as the generation of Aβ. CONCLUSIONS: The mechanism by which exogenous Aβ(1-42) induces BACE1 expression and Aβ generation is mediated by BACE1-AS. BACE1-AS is involved in the mechanism regulating BACE1 expression and Aβ generation in APPsw transgenic cells.
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spelling pubmed-67710942019-10-03 The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression Li, Fan Wang, Yun Yang, Hui Xu, Yingying Zhou, Xiaoyan Zhang, Xiao Xie, Zhaohong Bi, Jianzhong BMC Mol Biol Research Article BACKGROUND: The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1 mRNA and promotes the formation of Aβ. After the administration of Aβ(1-42) to SH-SY5Y cells and C57/BL6J mice, we detected the expression of BACE1-AS, BACE1 mRNA, and BACE1 protein, as well as the concentration of Aβ(1-40). Then, we silenced the expression of BACE1-AS in SH-SY5Y and 20E2 cells using siRNAs targeting BACE1-AS and detected its effects on the levels of the BACE1 mRNA and BACE1 protein and Aβ(1-40) generation. RESULTS: The administration of Aβ(1-42) increased the expression of BACE1-AS, BACE1 mRNA and protein, as well as the concentration of Aβ(1-40) in SH-SY5Y cells and the brains of C57BL/6J mice. Pretreatment with the BACE1-AS siRNA inhibited the effect of Aβ(1-42) on increasing the expression of BACE1-AS and BACE1, as well as the generation of Aβ. CONCLUSIONS: The mechanism by which exogenous Aβ(1-42) induces BACE1 expression and Aβ generation is mediated by BACE1-AS. BACE1-AS is involved in the mechanism regulating BACE1 expression and Aβ generation in APPsw transgenic cells. BioMed Central 2019-10-01 /pmc/articles/PMC6771094/ /pubmed/31570097 http://dx.doi.org/10.1186/s12867-019-0140-0 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Li, Fan
Wang, Yun
Yang, Hui
Xu, Yingying
Zhou, Xiaoyan
Zhang, Xiao
Xie, Zhaohong
Bi, Jianzhong
The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
title The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
title_full The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
title_fullStr The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
title_full_unstemmed The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
title_short The effect of BACE1-AS on β-amyloid generation by regulating BACE1 mRNA expression
title_sort effect of bace1-as on β-amyloid generation by regulating bace1 mrna expression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771094/
https://www.ncbi.nlm.nih.gov/pubmed/31570097
http://dx.doi.org/10.1186/s12867-019-0140-0
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