Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks

Here, we investigate the function of fission yeast Fun30/Smarcad1 family of SNF2 ATPase-dependent chromatin remodeling enzymes in DNA damage repair. There are three Fun30 homologues in fission yeast, Fft1, Fft2, and Fft3. We find that only Fft3 has a function in DNA repair and it is needed for singl...

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Detalles Bibliográficos
Autores principales: Ait-Saada, Anissia, Khorosjutina, Olga, Chen, Jiang, Kramarz, Karol, Maksimov, Vladimir, Svensson, J Peter, Lambert, Sarah, Ekwall, Karl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2019
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771652/
https://www.ncbi.nlm.nih.gov/pubmed/31575705
http://dx.doi.org/10.26508/lsa.201900433
Descripción
Sumario:Here, we investigate the function of fission yeast Fun30/Smarcad1 family of SNF2 ATPase-dependent chromatin remodeling enzymes in DNA damage repair. There are three Fun30 homologues in fission yeast, Fft1, Fft2, and Fft3. We find that only Fft3 has a function in DNA repair and it is needed for single-strand annealing of an induced double-strand break. Furthermore, we use an inducible replication fork barrier system to show that Fft3 has two distinct roles at blocked DNA replication forks. First, Fft3 is needed for the resection of nascent strands, and second, it is required to restart the blocked forks. The latter function is independent of its ATPase activity.

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