Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks

Here, we investigate the function of fission yeast Fun30/Smarcad1 family of SNF2 ATPase-dependent chromatin remodeling enzymes in DNA damage repair. There are three Fun30 homologues in fission yeast, Fft1, Fft2, and Fft3. We find that only Fft3 has a function in DNA repair and it is needed for singl...

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Autores principales: Ait-Saada, Anissia, Khorosjutina, Olga, Chen, Jiang, Kramarz, Karol, Maksimov, Vladimir, Svensson, J Peter, Lambert, Sarah, Ekwall, Karl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2019
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771652/
https://www.ncbi.nlm.nih.gov/pubmed/31575705
http://dx.doi.org/10.26508/lsa.201900433
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author Ait-Saada, Anissia
Khorosjutina, Olga
Chen, Jiang
Kramarz, Karol
Maksimov, Vladimir
Svensson, J Peter
Lambert, Sarah
Ekwall, Karl
author_facet Ait-Saada, Anissia
Khorosjutina, Olga
Chen, Jiang
Kramarz, Karol
Maksimov, Vladimir
Svensson, J Peter
Lambert, Sarah
Ekwall, Karl
author_sort Ait-Saada, Anissia
collection PubMed
description Here, we investigate the function of fission yeast Fun30/Smarcad1 family of SNF2 ATPase-dependent chromatin remodeling enzymes in DNA damage repair. There are three Fun30 homologues in fission yeast, Fft1, Fft2, and Fft3. We find that only Fft3 has a function in DNA repair and it is needed for single-strand annealing of an induced double-strand break. Furthermore, we use an inducible replication fork barrier system to show that Fft3 has two distinct roles at blocked DNA replication forks. First, Fft3 is needed for the resection of nascent strands, and second, it is required to restart the blocked forks. The latter function is independent of its ATPase activity.
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spelling pubmed-67716522019-10-02 Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks Ait-Saada, Anissia Khorosjutina, Olga Chen, Jiang Kramarz, Karol Maksimov, Vladimir Svensson, J Peter Lambert, Sarah Ekwall, Karl Life Sci Alliance Research Articles Here, we investigate the function of fission yeast Fun30/Smarcad1 family of SNF2 ATPase-dependent chromatin remodeling enzymes in DNA damage repair. There are three Fun30 homologues in fission yeast, Fft1, Fft2, and Fft3. We find that only Fft3 has a function in DNA repair and it is needed for single-strand annealing of an induced double-strand break. Furthermore, we use an inducible replication fork barrier system to show that Fft3 has two distinct roles at blocked DNA replication forks. First, Fft3 is needed for the resection of nascent strands, and second, it is required to restart the blocked forks. The latter function is independent of its ATPase activity. Life Science Alliance LLC 2019-10-01 /pmc/articles/PMC6771652/ /pubmed/31575705 http://dx.doi.org/10.26508/lsa.201900433 Text en © 2019 Ait-Saada et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Ait-Saada, Anissia
Khorosjutina, Olga
Chen, Jiang
Kramarz, Karol
Maksimov, Vladimir
Svensson, J Peter
Lambert, Sarah
Ekwall, Karl
Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks
title Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks
title_full Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks
title_fullStr Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks
title_full_unstemmed Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks
title_short Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks
title_sort chromatin remodeler fft3 plays a dual role at blocked dna replication forks
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771652/
https://www.ncbi.nlm.nih.gov/pubmed/31575705
http://dx.doi.org/10.26508/lsa.201900433
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