Cargando…
miR‐22 enhances the radiosensitivity of small‐cell lung cancer by targeting the WRNIP1
Small‐cell lung cancer (SCLC) is an aggressive malignancy characterized by high cellular proliferation and early distant metastasis. Our study aimed to explore the effect of miR‐22‐3p (miR‐22, for short) on SCLC radiosensitivity and its molecular mechanisms. The expression level of miR‐22 was evalua...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771739/ https://www.ncbi.nlm.nih.gov/pubmed/31190355 http://dx.doi.org/10.1002/jcb.29032 |
_version_ | 1783455756536774656 |
---|---|
author | Jiang, Wenhua Han, Xuemei Wang, Jingrui Wang, Lin Xu, Zanmei Wei, Qiao Zhang, Wenpan Wang, Haitao |
author_facet | Jiang, Wenhua Han, Xuemei Wang, Jingrui Wang, Lin Xu, Zanmei Wei, Qiao Zhang, Wenpan Wang, Haitao |
author_sort | Jiang, Wenhua |
collection | PubMed |
description | Small‐cell lung cancer (SCLC) is an aggressive malignancy characterized by high cellular proliferation and early distant metastasis. Our study aimed to explore the effect of miR‐22‐3p (miR‐22, for short) on SCLC radiosensitivity and its molecular mechanisms. The expression level of miR‐22 was evaluated in a human normal lung epithelial cell line and a human SCLC cell line, and cell apoptosis and migration were detected. The expression of the miR‐22 direct target WRNIP1 mRNA and protein were explored. Five differentially expressed genes were detected. The miR‐22 expression in NCI‐H446 was significantly decreased, and miR‐22 overexpression significantly promoted cell apoptosis. miR‐22 overexpression could significantly inhibit the cell migration of SCLC cells, and miR‐22 had a negative regulatory effect on WRNIP1 mRNA and protein levels. KLK8 was downregulated, and the messenger RNA (mRNA) of four other genes (PC, SCUBE1, STC1, and GPM6A) was upregulated mRNA in cells overexpressing miR‐22, which was in accordance with the bioinformatics analysis. miR‐22 could enhance the radiosensitivity of SCLC by targeting WRNIP1. |
format | Online Article Text |
id | pubmed-6771739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67717392019-10-07 miR‐22 enhances the radiosensitivity of small‐cell lung cancer by targeting the WRNIP1 Jiang, Wenhua Han, Xuemei Wang, Jingrui Wang, Lin Xu, Zanmei Wei, Qiao Zhang, Wenpan Wang, Haitao J Cell Biochem Research Articles Small‐cell lung cancer (SCLC) is an aggressive malignancy characterized by high cellular proliferation and early distant metastasis. Our study aimed to explore the effect of miR‐22‐3p (miR‐22, for short) on SCLC radiosensitivity and its molecular mechanisms. The expression level of miR‐22 was evaluated in a human normal lung epithelial cell line and a human SCLC cell line, and cell apoptosis and migration were detected. The expression of the miR‐22 direct target WRNIP1 mRNA and protein were explored. Five differentially expressed genes were detected. The miR‐22 expression in NCI‐H446 was significantly decreased, and miR‐22 overexpression significantly promoted cell apoptosis. miR‐22 overexpression could significantly inhibit the cell migration of SCLC cells, and miR‐22 had a negative regulatory effect on WRNIP1 mRNA and protein levels. KLK8 was downregulated, and the messenger RNA (mRNA) of four other genes (PC, SCUBE1, STC1, and GPM6A) was upregulated mRNA in cells overexpressing miR‐22, which was in accordance with the bioinformatics analysis. miR‐22 could enhance the radiosensitivity of SCLC by targeting WRNIP1. John Wiley and Sons Inc. 2019-06-12 2019-10 /pmc/articles/PMC6771739/ /pubmed/31190355 http://dx.doi.org/10.1002/jcb.29032 Text en © 2019 The Authors. Journal of Cellular Biochemistry Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Jiang, Wenhua Han, Xuemei Wang, Jingrui Wang, Lin Xu, Zanmei Wei, Qiao Zhang, Wenpan Wang, Haitao miR‐22 enhances the radiosensitivity of small‐cell lung cancer by targeting the WRNIP1 |
title |
miR‐22
enhances the radiosensitivity of small‐cell lung cancer by targeting the
WRNIP1
|
title_full |
miR‐22
enhances the radiosensitivity of small‐cell lung cancer by targeting the
WRNIP1
|
title_fullStr |
miR‐22
enhances the radiosensitivity of small‐cell lung cancer by targeting the
WRNIP1
|
title_full_unstemmed |
miR‐22
enhances the radiosensitivity of small‐cell lung cancer by targeting the
WRNIP1
|
title_short |
miR‐22
enhances the radiosensitivity of small‐cell lung cancer by targeting the
WRNIP1
|
title_sort | mir‐22
enhances the radiosensitivity of small‐cell lung cancer by targeting the
wrnip1 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771739/ https://www.ncbi.nlm.nih.gov/pubmed/31190355 http://dx.doi.org/10.1002/jcb.29032 |
work_keys_str_mv | AT jiangwenhua mir22enhancestheradiosensitivityofsmallcelllungcancerbytargetingthewrnip1 AT hanxuemei mir22enhancestheradiosensitivityofsmallcelllungcancerbytargetingthewrnip1 AT wangjingrui mir22enhancestheradiosensitivityofsmallcelllungcancerbytargetingthewrnip1 AT wanglin mir22enhancestheradiosensitivityofsmallcelllungcancerbytargetingthewrnip1 AT xuzanmei mir22enhancestheradiosensitivityofsmallcelllungcancerbytargetingthewrnip1 AT weiqiao mir22enhancestheradiosensitivityofsmallcelllungcancerbytargetingthewrnip1 AT zhangwenpan mir22enhancestheradiosensitivityofsmallcelllungcancerbytargetingthewrnip1 AT wanghaitao mir22enhancestheradiosensitivityofsmallcelllungcancerbytargetingthewrnip1 |