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Identification of a crucial amino acid implicated in the hydroxylation/desaturation ratio of CpFAH12 bifunctional hydroxylase
Claviceps purpurea bifunctional Δ12‐hydroxylase/desaturase, CpFAH12, and monofunctional desaturase CpFAD2, share 86% of sequence identity. To identify the underlying determinants of the hydroxylation/desaturation specificity, chimeras of these two enzymes were tested for their fatty acid production...
Autores principales: | Robin, Julien, Gueroult, Marc, Cheikhrouhou, Randa, Guicherd, Marie, Borsenberger, Vinciane, Marty, Alain, Bordes, Florence |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771796/ https://www.ncbi.nlm.nih.gov/pubmed/31282998 http://dx.doi.org/10.1002/bit.27102 |
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