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Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses

Late embryogenesis abundant (LEA) proteins are widely known to be present in higher plants and are believed to play important functional roles in embryonic development and abiotic stress responses. However, there is a current lack of systematic analyses on the LEA protein gene family in tea plant. I...

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Autores principales: Jin, Xiaofang, Cao, Dan, Wang, Zhongjie, Ma, Linlong, Tian, Kunhong, Liu, Yanli, Gong, Ziming, Zhu, Xiangxiang, Jiang, Changjun, Li, Yeyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6773783/
https://www.ncbi.nlm.nih.gov/pubmed/31575979
http://dx.doi.org/10.1038/s41598-019-50645-8
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author Jin, Xiaofang
Cao, Dan
Wang, Zhongjie
Ma, Linlong
Tian, Kunhong
Liu, Yanli
Gong, Ziming
Zhu, Xiangxiang
Jiang, Changjun
Li, Yeyun
author_facet Jin, Xiaofang
Cao, Dan
Wang, Zhongjie
Ma, Linlong
Tian, Kunhong
Liu, Yanli
Gong, Ziming
Zhu, Xiangxiang
Jiang, Changjun
Li, Yeyun
author_sort Jin, Xiaofang
collection PubMed
description Late embryogenesis abundant (LEA) proteins are widely known to be present in higher plants and are believed to play important functional roles in embryonic development and abiotic stress responses. However, there is a current lack of systematic analyses on the LEA protein gene family in tea plant. In this study, a total of 48 LEA genes were identified using Hidden Markov Model profiles in C. sinensis, and were classified into seven distinct groups based on their conserved domains and phylogenetic relationships. Genes in the CsLEA_2 group were found to be the most abundant. Gene expression analyses revealed that all the identified CsLEA genes were expressed in at least one tissue, and most had higher expression levels in the root or seed relative to other tested tissues. Nearly all the CsLEA genes were found to be involved in seed development, and thirty-nine might play an important role in tea seed maturation concurrent with dehydration. However, only sixteen CsLEA genes were involved in seed desiccation, and furthermore, most were suppressed. Additionally, forty-six CsLEA genes could be induced by at least one of the tested stress treatments, and they were especially sensitive to high temperature stress. Furthermore, it was found that eleven CsLEA genes were involved in tea plant in response to all tested abiotic stresses. Overall, this study provides new insights into the formation of CsLEA gene family members and improves our understanding on the potential roles of these genes in normal development processes and abiotic stress responses in tea plant, particularly during seed development and desiccation. These results are beneficial for future functional studies of CsLEA genes that will help preserve the recalcitrant tea seeds for a long time and genetically improve tea plant.
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spelling pubmed-67737832019-10-04 Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses Jin, Xiaofang Cao, Dan Wang, Zhongjie Ma, Linlong Tian, Kunhong Liu, Yanli Gong, Ziming Zhu, Xiangxiang Jiang, Changjun Li, Yeyun Sci Rep Article Late embryogenesis abundant (LEA) proteins are widely known to be present in higher plants and are believed to play important functional roles in embryonic development and abiotic stress responses. However, there is a current lack of systematic analyses on the LEA protein gene family in tea plant. In this study, a total of 48 LEA genes were identified using Hidden Markov Model profiles in C. sinensis, and were classified into seven distinct groups based on their conserved domains and phylogenetic relationships. Genes in the CsLEA_2 group were found to be the most abundant. Gene expression analyses revealed that all the identified CsLEA genes were expressed in at least one tissue, and most had higher expression levels in the root or seed relative to other tested tissues. Nearly all the CsLEA genes were found to be involved in seed development, and thirty-nine might play an important role in tea seed maturation concurrent with dehydration. However, only sixteen CsLEA genes were involved in seed desiccation, and furthermore, most were suppressed. Additionally, forty-six CsLEA genes could be induced by at least one of the tested stress treatments, and they were especially sensitive to high temperature stress. Furthermore, it was found that eleven CsLEA genes were involved in tea plant in response to all tested abiotic stresses. Overall, this study provides new insights into the formation of CsLEA gene family members and improves our understanding on the potential roles of these genes in normal development processes and abiotic stress responses in tea plant, particularly during seed development and desiccation. These results are beneficial for future functional studies of CsLEA genes that will help preserve the recalcitrant tea seeds for a long time and genetically improve tea plant. Nature Publishing Group UK 2019-10-01 /pmc/articles/PMC6773783/ /pubmed/31575979 http://dx.doi.org/10.1038/s41598-019-50645-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Jin, Xiaofang
Cao, Dan
Wang, Zhongjie
Ma, Linlong
Tian, Kunhong
Liu, Yanli
Gong, Ziming
Zhu, Xiangxiang
Jiang, Changjun
Li, Yeyun
Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses
title Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses
title_full Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses
title_fullStr Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses
title_full_unstemmed Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses
title_short Genome-wide identification and expression analyses of the LEA protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses
title_sort genome-wide identification and expression analyses of the lea protein gene family in tea plant reveal their involvement in seed development and abiotic stress responses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6773783/
https://www.ncbi.nlm.nih.gov/pubmed/31575979
http://dx.doi.org/10.1038/s41598-019-50645-8
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