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Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass

European sea bass (Dicentrarchus labrax) is severely affected by nervous necrosis disease, caused by nervous necrosis virus (NNV). Two out of the four genotypes of this virus (red-spotted grouper nervous necrosis virus, RGNNV; and striped jack nervous necrosis virus, SJNNV) have been detected in sea...

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Autores principales: Moreno, Patricia, Souto, Sandra, Leiva-Rebollo, Rocio, Borrego, Juan J., Bandín, Isabel, Alonso, M. Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6773868/
https://www.ncbi.nlm.nih.gov/pubmed/31575937
http://dx.doi.org/10.1038/s41598-019-50622-1
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author Moreno, Patricia
Souto, Sandra
Leiva-Rebollo, Rocio
Borrego, Juan J.
Bandín, Isabel
Alonso, M. Carmen
author_facet Moreno, Patricia
Souto, Sandra
Leiva-Rebollo, Rocio
Borrego, Juan J.
Bandín, Isabel
Alonso, M. Carmen
author_sort Moreno, Patricia
collection PubMed
description European sea bass (Dicentrarchus labrax) is severely affected by nervous necrosis disease, caused by nervous necrosis virus (NNV). Two out of the four genotypes of this virus (red-spotted grouper nervous necrosis virus, RGNNV; and striped jack nervous necrosis virus, SJNNV) have been detected in sea bass, although showing different levels of virulence to this fish species. Thus, sea bass is highly susceptible to RGNNV, whereas outbreaks caused by SJNNV have not been reported in this fish species. The role of the capsid protein (Cp) amino acids 247 and 270 in the virulence of a RGNNV isolate to sea bass has been evaluated by the generation of recombinant RGNNV viruses harbouring SJNNV-type amino acids in the above mentioned positions (Mut247Dl965, Mut270Dl965 and Mut247 + 270Dl965). Viral in vitro and in vivo replication, virus virulence and fish immune response triggered by these viruses have been analysed. Mutated viruses replicated on E-11 cells, although showing some differences compared to the wild type virus, suggesting that the mutations can affect the viral cell recognition and entry. In vivo, fish mortality caused by mutated viruses was 75% lower, and viral replication in sea bass brain was altered compared to non-mutated virus. Regarding sea bass immune response, mutated viruses triggered a lower induction of IFN I system and inflammatory response-related genes. Furthermore, mutations caused changes in viral serological properties (especially the mutation in amino acid 270), inducing higher seroconversion and changing antigen recognition.
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spelling pubmed-67738682019-10-04 Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass Moreno, Patricia Souto, Sandra Leiva-Rebollo, Rocio Borrego, Juan J. Bandín, Isabel Alonso, M. Carmen Sci Rep Article European sea bass (Dicentrarchus labrax) is severely affected by nervous necrosis disease, caused by nervous necrosis virus (NNV). Two out of the four genotypes of this virus (red-spotted grouper nervous necrosis virus, RGNNV; and striped jack nervous necrosis virus, SJNNV) have been detected in sea bass, although showing different levels of virulence to this fish species. Thus, sea bass is highly susceptible to RGNNV, whereas outbreaks caused by SJNNV have not been reported in this fish species. The role of the capsid protein (Cp) amino acids 247 and 270 in the virulence of a RGNNV isolate to sea bass has been evaluated by the generation of recombinant RGNNV viruses harbouring SJNNV-type amino acids in the above mentioned positions (Mut247Dl965, Mut270Dl965 and Mut247 + 270Dl965). Viral in vitro and in vivo replication, virus virulence and fish immune response triggered by these viruses have been analysed. Mutated viruses replicated on E-11 cells, although showing some differences compared to the wild type virus, suggesting that the mutations can affect the viral cell recognition and entry. In vivo, fish mortality caused by mutated viruses was 75% lower, and viral replication in sea bass brain was altered compared to non-mutated virus. Regarding sea bass immune response, mutated viruses triggered a lower induction of IFN I system and inflammatory response-related genes. Furthermore, mutations caused changes in viral serological properties (especially the mutation in amino acid 270), inducing higher seroconversion and changing antigen recognition. Nature Publishing Group UK 2019-10-01 /pmc/articles/PMC6773868/ /pubmed/31575937 http://dx.doi.org/10.1038/s41598-019-50622-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Moreno, Patricia
Souto, Sandra
Leiva-Rebollo, Rocio
Borrego, Juan J.
Bandín, Isabel
Alonso, M. Carmen
Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass
title Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass
title_full Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass
title_fullStr Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass
title_full_unstemmed Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass
title_short Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass
title_sort capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to european sea bass
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6773868/
https://www.ncbi.nlm.nih.gov/pubmed/31575937
http://dx.doi.org/10.1038/s41598-019-50622-1
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