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MicroRNA-155-3p promotes breast cancer progression through down-regulating CADM1

BACKGROUND/PURPOSE: Cell adhesion molecule 1 (CADM1) functions as a tumor suppressor and has been identified to be frequently inactivated in breast cancer, and closely associated with patients’ poor prognosis and advanced TNM stage. However, the mechanisms underlying CADM1 in breast cancer progressi...

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Autores principales: Zhang, Guochao, Zhong, Lele, Luo, Hao, Wang, Shibing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6773971/
https://www.ncbi.nlm.nih.gov/pubmed/31579252
http://dx.doi.org/10.2147/OTT.S206180
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author Zhang, Guochao
Zhong, Lele
Luo, Hao
Wang, Shibing
author_facet Zhang, Guochao
Zhong, Lele
Luo, Hao
Wang, Shibing
author_sort Zhang, Guochao
collection PubMed
description BACKGROUND/PURPOSE: Cell adhesion molecule 1 (CADM1) functions as a tumor suppressor and has been identified to be frequently inactivated in breast cancer, and closely associated with patients’ poor prognosis and advanced TNM stage. However, the mechanisms underlying CADM1 in breast cancer progression remains incompletely clear. miR-155, a predicted modulator of CADM1 was reported to be overexpressed in breast cancer, and its high expression level was closely related to the malignant progression of breast cancer. The present study aimed to explore whether miR-155-3p could modulate CADM1 expression and then involved in the progression of breast cancer. METHODS: The expression patterns of miR-155-3p in breast cancer tissues and cell lines were determined by RT-PCR technology. The relationship between CADM1 and miR-155-3p were determined by the luciferase gene reporter and Western Blot (WB) assays. Cell proliferation, apoptosis rates and tumorigenesis were determined by CCK-8, flow cytometry and in vivo xenotransplanation experiments, respectively. RESULTS: miR-155-3p was up-regulated in breast cancer tissues and cells when compared to the adjacent normal tissues and normal breast MCF 10A cells. The mRNA and protein levels of CADM1 showed opposite expression patterns to that of miR-155-3p expression detected, and miR-155-3p could negatively regulate CADM1 expression in breast cancer MCF-7 cells. Moreover, gain-of function assay showed that overexpression of miR-155-3p promoted cell proliferation, tumorigenesis and repressed cell apoptosis, but these effects were all significantly impaired when the cells were simultaneously transfected with OE-CADM1, the overexpressing vector of CADM1. CONCLUSION: This study revealed that miR-155-3p could accelerate the progression of breast cancer via down-regulation of CADM1 expression.
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spelling pubmed-67739712019-10-02 MicroRNA-155-3p promotes breast cancer progression through down-regulating CADM1 Zhang, Guochao Zhong, Lele Luo, Hao Wang, Shibing Onco Targets Ther Original Research BACKGROUND/PURPOSE: Cell adhesion molecule 1 (CADM1) functions as a tumor suppressor and has been identified to be frequently inactivated in breast cancer, and closely associated with patients’ poor prognosis and advanced TNM stage. However, the mechanisms underlying CADM1 in breast cancer progression remains incompletely clear. miR-155, a predicted modulator of CADM1 was reported to be overexpressed in breast cancer, and its high expression level was closely related to the malignant progression of breast cancer. The present study aimed to explore whether miR-155-3p could modulate CADM1 expression and then involved in the progression of breast cancer. METHODS: The expression patterns of miR-155-3p in breast cancer tissues and cell lines were determined by RT-PCR technology. The relationship between CADM1 and miR-155-3p were determined by the luciferase gene reporter and Western Blot (WB) assays. Cell proliferation, apoptosis rates and tumorigenesis were determined by CCK-8, flow cytometry and in vivo xenotransplanation experiments, respectively. RESULTS: miR-155-3p was up-regulated in breast cancer tissues and cells when compared to the adjacent normal tissues and normal breast MCF 10A cells. The mRNA and protein levels of CADM1 showed opposite expression patterns to that of miR-155-3p expression detected, and miR-155-3p could negatively regulate CADM1 expression in breast cancer MCF-7 cells. Moreover, gain-of function assay showed that overexpression of miR-155-3p promoted cell proliferation, tumorigenesis and repressed cell apoptosis, but these effects were all significantly impaired when the cells were simultaneously transfected with OE-CADM1, the overexpressing vector of CADM1. CONCLUSION: This study revealed that miR-155-3p could accelerate the progression of breast cancer via down-regulation of CADM1 expression. Dove 2019-09-27 /pmc/articles/PMC6773971/ /pubmed/31579252 http://dx.doi.org/10.2147/OTT.S206180 Text en © 2019 Zhang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Zhang, Guochao
Zhong, Lele
Luo, Hao
Wang, Shibing
MicroRNA-155-3p promotes breast cancer progression through down-regulating CADM1
title MicroRNA-155-3p promotes breast cancer progression through down-regulating CADM1
title_full MicroRNA-155-3p promotes breast cancer progression through down-regulating CADM1
title_fullStr MicroRNA-155-3p promotes breast cancer progression through down-regulating CADM1
title_full_unstemmed MicroRNA-155-3p promotes breast cancer progression through down-regulating CADM1
title_short MicroRNA-155-3p promotes breast cancer progression through down-regulating CADM1
title_sort microrna-155-3p promotes breast cancer progression through down-regulating cadm1
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6773971/
https://www.ncbi.nlm.nih.gov/pubmed/31579252
http://dx.doi.org/10.2147/OTT.S206180
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