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Visualizing the Fate of Intra‐Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near‐Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears

Mesenchymal stem cells (MSCs) are capable of exerting strong therapeutic potential for the treatment of supraspinatus tendon tear. However, MSC therapy remains underutilized and perhaps underrated due to the limited evidence of dynamic visualization of cellular behavior in vivo. Here, second near‐in...

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Autores principales: Yang, Yimeng, Chen, Jun, Shang, Xiliang, Feng, Zhujun, Chen, Chen, Lu, Jingyi, Cai, Jiangyu, Chen, Yuzhou, Zhang, Jian, Hao, Yuefeng, Yang, Xing, Li, Yunxia, Chen, Shiyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6774022/
https://www.ncbi.nlm.nih.gov/pubmed/31592419
http://dx.doi.org/10.1002/advs.201901018
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author Yang, Yimeng
Chen, Jun
Shang, Xiliang
Feng, Zhujun
Chen, Chen
Lu, Jingyi
Cai, Jiangyu
Chen, Yuzhou
Zhang, Jian
Hao, Yuefeng
Yang, Xing
Li, Yunxia
Chen, Shiyi
author_facet Yang, Yimeng
Chen, Jun
Shang, Xiliang
Feng, Zhujun
Chen, Chen
Lu, Jingyi
Cai, Jiangyu
Chen, Yuzhou
Zhang, Jian
Hao, Yuefeng
Yang, Xing
Li, Yunxia
Chen, Shiyi
author_sort Yang, Yimeng
collection PubMed
description Mesenchymal stem cells (MSCs) are capable of exerting strong therapeutic potential for the treatment of supraspinatus tendon tear. However, MSC therapy remains underutilized and perhaps underrated due to the limited evidence of dynamic visualization of cellular behavior in vivo. Here, second near‐infrared fluorescence imaging with biocompatible PbS quantum dots (QDs) provides a cellular migration map and information on the biodistribution and clearance processes of three densities of intra‐articularly injected, labeled MSCs to treat supraspinatus tendon tear in mice. Intra‐articular injection avoids entrapment of MSCs by filter organs and reduces the QD‐induced organ toxicity. Notably, the MSCs share a similar migration direction, but the moderate density group is somewhat more efficient, showing the longest residence time and highest cell retention rate around the footprint during the repair stage. Furthermore, quantitative kinetic investigation demonstrates that labeled MSCs are cleared by feces and urine. Histomorphometric analysis demonstrates that the moderate density group achieves maximum therapeutic effect and labeled MSCs do not induce any injury or inflammation to major organs, which suggests that administration of too many or few MSCs may decrease their effectiveness. Such an imaging approach provides spatiotemporal evidence for response to MSC therapy in vivo, facilitating the optimization of MSC therapy.
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spelling pubmed-67740222019-10-07 Visualizing the Fate of Intra‐Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near‐Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears Yang, Yimeng Chen, Jun Shang, Xiliang Feng, Zhujun Chen, Chen Lu, Jingyi Cai, Jiangyu Chen, Yuzhou Zhang, Jian Hao, Yuefeng Yang, Xing Li, Yunxia Chen, Shiyi Adv Sci (Weinh) Full Papers Mesenchymal stem cells (MSCs) are capable of exerting strong therapeutic potential for the treatment of supraspinatus tendon tear. However, MSC therapy remains underutilized and perhaps underrated due to the limited evidence of dynamic visualization of cellular behavior in vivo. Here, second near‐infrared fluorescence imaging with biocompatible PbS quantum dots (QDs) provides a cellular migration map and information on the biodistribution and clearance processes of three densities of intra‐articularly injected, labeled MSCs to treat supraspinatus tendon tear in mice. Intra‐articular injection avoids entrapment of MSCs by filter organs and reduces the QD‐induced organ toxicity. Notably, the MSCs share a similar migration direction, but the moderate density group is somewhat more efficient, showing the longest residence time and highest cell retention rate around the footprint during the repair stage. Furthermore, quantitative kinetic investigation demonstrates that labeled MSCs are cleared by feces and urine. Histomorphometric analysis demonstrates that the moderate density group achieves maximum therapeutic effect and labeled MSCs do not induce any injury or inflammation to major organs, which suggests that administration of too many or few MSCs may decrease their effectiveness. Such an imaging approach provides spatiotemporal evidence for response to MSC therapy in vivo, facilitating the optimization of MSC therapy. John Wiley and Sons Inc. 2019-08-01 /pmc/articles/PMC6774022/ /pubmed/31592419 http://dx.doi.org/10.1002/advs.201901018 Text en © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Yang, Yimeng
Chen, Jun
Shang, Xiliang
Feng, Zhujun
Chen, Chen
Lu, Jingyi
Cai, Jiangyu
Chen, Yuzhou
Zhang, Jian
Hao, Yuefeng
Yang, Xing
Li, Yunxia
Chen, Shiyi
Visualizing the Fate of Intra‐Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near‐Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears
title Visualizing the Fate of Intra‐Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near‐Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears
title_full Visualizing the Fate of Intra‐Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near‐Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears
title_fullStr Visualizing the Fate of Intra‐Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near‐Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears
title_full_unstemmed Visualizing the Fate of Intra‐Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near‐Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears
title_short Visualizing the Fate of Intra‐Articular Injected Mesenchymal Stem Cells In Vivo in the Second Near‐Infrared Window for the Effective Treatment of Supraspinatus Tendon Tears
title_sort visualizing the fate of intra‐articular injected mesenchymal stem cells in vivo in the second near‐infrared window for the effective treatment of supraspinatus tendon tears
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6774022/
https://www.ncbi.nlm.nih.gov/pubmed/31592419
http://dx.doi.org/10.1002/advs.201901018
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