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Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins
Membrane proteins mediate a number of cellular functions and are associated with several diseases and also play a crucial role in pathogenicity. Due to their importance in cellular structure and function, they are important drug targets for ~60% of drugs available in the market. Despite the technolo...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6774292/ https://www.ncbi.nlm.nih.gov/pubmed/31608290 http://dx.doi.org/10.3389/fmolb.2019.00096 |
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author | Raghuraman, H. Chatterjee, Satyaki Das, Anindita |
author_facet | Raghuraman, H. Chatterjee, Satyaki Das, Anindita |
author_sort | Raghuraman, H. |
collection | PubMed |
description | Membrane proteins mediate a number of cellular functions and are associated with several diseases and also play a crucial role in pathogenicity. Due to their importance in cellular structure and function, they are important drug targets for ~60% of drugs available in the market. Despite the technological advancement and recent successful outcomes in determining the high-resolution structural snapshot of membrane proteins, the mechanistic details underlining the complex functionalities of membrane proteins is least understood. This is largely due to lack of structural dynamics information pertaining to different functional states of membrane proteins in a membrane environment. Fluorescence spectroscopy is a widely used technique in the analysis of functionally-relevant structure and dynamics of membrane protein. This review is focused on various site-directed fluorescence (SDFL) approaches and their applications to explore structural information, conformational changes, hydration dynamics, and lipid-protein interactions of important classes of membrane proteins that include the pore-forming peptides/proteins, ion channels/transporters and G-protein coupled receptors. |
format | Online Article Text |
id | pubmed-6774292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67742922019-10-13 Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins Raghuraman, H. Chatterjee, Satyaki Das, Anindita Front Mol Biosci Molecular Biosciences Membrane proteins mediate a number of cellular functions and are associated with several diseases and also play a crucial role in pathogenicity. Due to their importance in cellular structure and function, they are important drug targets for ~60% of drugs available in the market. Despite the technological advancement and recent successful outcomes in determining the high-resolution structural snapshot of membrane proteins, the mechanistic details underlining the complex functionalities of membrane proteins is least understood. This is largely due to lack of structural dynamics information pertaining to different functional states of membrane proteins in a membrane environment. Fluorescence spectroscopy is a widely used technique in the analysis of functionally-relevant structure and dynamics of membrane protein. This review is focused on various site-directed fluorescence (SDFL) approaches and their applications to explore structural information, conformational changes, hydration dynamics, and lipid-protein interactions of important classes of membrane proteins that include the pore-forming peptides/proteins, ion channels/transporters and G-protein coupled receptors. Frontiers Media S.A. 2019-09-25 /pmc/articles/PMC6774292/ /pubmed/31608290 http://dx.doi.org/10.3389/fmolb.2019.00096 Text en Copyright © 2019 Raghuraman, Chatterjee and Das. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Raghuraman, H. Chatterjee, Satyaki Das, Anindita Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins |
title | Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins |
title_full | Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins |
title_fullStr | Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins |
title_full_unstemmed | Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins |
title_short | Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins |
title_sort | site-directed fluorescence approaches for dynamic structural biology of membrane peptides and proteins |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6774292/ https://www.ncbi.nlm.nih.gov/pubmed/31608290 http://dx.doi.org/10.3389/fmolb.2019.00096 |
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