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Bacterial diversity in Icelandic cold spring sources and in relation to the groundwater amphipod Crangonyx islandicus
Crangonyx islandicus is a groundwater amphipod endemic to Iceland, considered to have survived the Ice Ages in subglacial refugia. Currently the species is found in spring sources in lava fields along the tectonic plate boundary of the country. The discovery of a groundwater species in this inaccess...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6774475/ https://www.ncbi.nlm.nih.gov/pubmed/31577799 http://dx.doi.org/10.1371/journal.pone.0222527 |
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author | Guðmundsdóttir, Ragnhildur Kreiling, Agnes-Katharina Kristjánsson, Bjarni Kristófer Marteinsson, Viggó Þór Pálsson, Snæbjörn |
author_facet | Guðmundsdóttir, Ragnhildur Kreiling, Agnes-Katharina Kristjánsson, Bjarni Kristófer Marteinsson, Viggó Þór Pálsson, Snæbjörn |
author_sort | Guðmundsdóttir, Ragnhildur |
collection | PubMed |
description | Crangonyx islandicus is a groundwater amphipod endemic to Iceland, considered to have survived the Ice Ages in subglacial refugia. Currently the species is found in spring sources in lava fields along the tectonic plate boundary of the country. The discovery of a groundwater species in this inaccessible habitat indicates a hidden ecosystem possibly based on chemoautotrophic microorganisms as primary producers. To explore this spring ecosystem, we assessed its microbial diversity and analysed whether and how the diversity varied between the amphipods and the spring water, and if was dependent on environmental factors and geological settings. Isolated DNA from spring water and from amphipods was analysed using metabarcoding methods, targeting the 16S rRNA gene. Two genera of bacteria, Halomonas and Shewanella were dominating in the amphipod samples in terms of relative abundance, but not in the groundwater samples where Flavobacterium, Pseudomonas and Alkanindiges among others were dominating. The richness of the bacteria taxa in the microbial community of the groundwater spring sources was shaped by pH level and the beta diversity was shaped by geographic locations. |
format | Online Article Text |
id | pubmed-6774475 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-67744752019-10-12 Bacterial diversity in Icelandic cold spring sources and in relation to the groundwater amphipod Crangonyx islandicus Guðmundsdóttir, Ragnhildur Kreiling, Agnes-Katharina Kristjánsson, Bjarni Kristófer Marteinsson, Viggó Þór Pálsson, Snæbjörn PLoS One Research Article Crangonyx islandicus is a groundwater amphipod endemic to Iceland, considered to have survived the Ice Ages in subglacial refugia. Currently the species is found in spring sources in lava fields along the tectonic plate boundary of the country. The discovery of a groundwater species in this inaccessible habitat indicates a hidden ecosystem possibly based on chemoautotrophic microorganisms as primary producers. To explore this spring ecosystem, we assessed its microbial diversity and analysed whether and how the diversity varied between the amphipods and the spring water, and if was dependent on environmental factors and geological settings. Isolated DNA from spring water and from amphipods was analysed using metabarcoding methods, targeting the 16S rRNA gene. Two genera of bacteria, Halomonas and Shewanella were dominating in the amphipod samples in terms of relative abundance, but not in the groundwater samples where Flavobacterium, Pseudomonas and Alkanindiges among others were dominating. The richness of the bacteria taxa in the microbial community of the groundwater spring sources was shaped by pH level and the beta diversity was shaped by geographic locations. Public Library of Science 2019-10-02 /pmc/articles/PMC6774475/ /pubmed/31577799 http://dx.doi.org/10.1371/journal.pone.0222527 Text en © 2019 Guðmundsdóttir et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Guðmundsdóttir, Ragnhildur Kreiling, Agnes-Katharina Kristjánsson, Bjarni Kristófer Marteinsson, Viggó Þór Pálsson, Snæbjörn Bacterial diversity in Icelandic cold spring sources and in relation to the groundwater amphipod Crangonyx islandicus |
title | Bacterial diversity in Icelandic cold spring sources and in relation to the groundwater amphipod Crangonyx islandicus |
title_full | Bacterial diversity in Icelandic cold spring sources and in relation to the groundwater amphipod Crangonyx islandicus |
title_fullStr | Bacterial diversity in Icelandic cold spring sources and in relation to the groundwater amphipod Crangonyx islandicus |
title_full_unstemmed | Bacterial diversity in Icelandic cold spring sources and in relation to the groundwater amphipod Crangonyx islandicus |
title_short | Bacterial diversity in Icelandic cold spring sources and in relation to the groundwater amphipod Crangonyx islandicus |
title_sort | bacterial diversity in icelandic cold spring sources and in relation to the groundwater amphipod crangonyx islandicus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6774475/ https://www.ncbi.nlm.nih.gov/pubmed/31577799 http://dx.doi.org/10.1371/journal.pone.0222527 |
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