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Optimised insert design for improved single-molecule imaging and quantification through CRISPR-Cas9 mediated knock-in

The use of CRISPR-Cas9 genome editing to introduce endogenously expressed tags has the potential to address a number of the classical limitations of single molecule localisation microscopy. In this work we present the first systematic comparison of inserts introduced through CRISPR-knock in, with th...

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Detalles Bibliográficos
Autores principales:	Khan, Abdullah O., White, Carl W., Pike, Jeremy A., Yule, Jack, Slater, Alexandre, Hill, Stephen J., Poulter, Natalie S., Thomas, Steven G., Morgan, Neil V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775134/ https://www.ncbi.nlm.nih.gov/pubmed/31578415 http://dx.doi.org/10.1038/s41598-019-50733-9

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