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Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane
Background: This study investigated the effects of propofol and isoflurane on endoplasmic reticulum (ER) stress in an animal model under general anaesthesia. Methods: Rats were randomly divided into Propofol and Isoflurane groups. Anaesthesia was maintained with propofol for Propofol group or isoflu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775274/ https://www.ncbi.nlm.nih.gov/pubmed/31588195 http://dx.doi.org/10.7150/ijms.36265 |
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author | Seo, Eun-Hye Piao, Liyun Park, Hyun-Jun Lee, Ji Yeon Sa, Mijung Oh, Chung-Sik Lee, Seung-Hyun Kim, Seong-Hyop |
author_facet | Seo, Eun-Hye Piao, Liyun Park, Hyun-Jun Lee, Ji Yeon Sa, Mijung Oh, Chung-Sik Lee, Seung-Hyun Kim, Seong-Hyop |
author_sort | Seo, Eun-Hye |
collection | PubMed |
description | Background: This study investigated the effects of propofol and isoflurane on endoplasmic reticulum (ER) stress in an animal model under general anaesthesia. Methods: Rats were randomly divided into Propofol and Isoflurane groups. Anaesthesia was maintained with propofol for Propofol group or isoflurane for Isoflurane group during 3 h. ER stress from lymphocytes in blood and tissues was evaluated between two groups after euthanasia. Reactive oxygen species (ROS) from lymphocytes in blood and tissues, and cytokines in blood were also checked. An immunohistochemical assay for ER stress marker from tissues was performed. Results: After anaesthesia, the levels of CCAAT-enhancer-binding protein homologous proteins (CHOP) in blood and liver were significantly higher in Isoflurane group, compared to Propofol group [blood, 31,499 ± 4,934 (30,733, 26,441-38,807) mean fluorescence intensity (MFI) in Isoflurane group vs. 20,595 ± 1,838 (20,780, 18,866-22,232) MFI in Propofol group, p = 0.002; liver, 28,342 ± 5,535 (29,421, 23,388-32,756) MFI in Isoflurane group vs. 20,004 ± 2,155 (19,244, 18,197-22,191) MFI in Propofol group, p = 0.020]. ROS in blood was significantly higher in Isoflurane group, compared to Propofol group. However, cytokines in blood and immunohistochemical assays in tissues were similar between groups. Conclusion: Significant higher of ER stress from blood and liver were observed in rats under anaesthesia with isoflurane, compared to those that received propofol. ROS from blood also showed significant higher under anaesthesia with isoflurane. However, these findings were not associated with any changes in cytokines in blood or immunohistochemical assay in tissues. |
format | Online Article Text |
id | pubmed-6775274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-67752742019-10-04 Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane Seo, Eun-Hye Piao, Liyun Park, Hyun-Jun Lee, Ji Yeon Sa, Mijung Oh, Chung-Sik Lee, Seung-Hyun Kim, Seong-Hyop Int J Med Sci Research Paper Background: This study investigated the effects of propofol and isoflurane on endoplasmic reticulum (ER) stress in an animal model under general anaesthesia. Methods: Rats were randomly divided into Propofol and Isoflurane groups. Anaesthesia was maintained with propofol for Propofol group or isoflurane for Isoflurane group during 3 h. ER stress from lymphocytes in blood and tissues was evaluated between two groups after euthanasia. Reactive oxygen species (ROS) from lymphocytes in blood and tissues, and cytokines in blood were also checked. An immunohistochemical assay for ER stress marker from tissues was performed. Results: After anaesthesia, the levels of CCAAT-enhancer-binding protein homologous proteins (CHOP) in blood and liver were significantly higher in Isoflurane group, compared to Propofol group [blood, 31,499 ± 4,934 (30,733, 26,441-38,807) mean fluorescence intensity (MFI) in Isoflurane group vs. 20,595 ± 1,838 (20,780, 18,866-22,232) MFI in Propofol group, p = 0.002; liver, 28,342 ± 5,535 (29,421, 23,388-32,756) MFI in Isoflurane group vs. 20,004 ± 2,155 (19,244, 18,197-22,191) MFI in Propofol group, p = 0.020]. ROS in blood was significantly higher in Isoflurane group, compared to Propofol group. However, cytokines in blood and immunohistochemical assays in tissues were similar between groups. Conclusion: Significant higher of ER stress from blood and liver were observed in rats under anaesthesia with isoflurane, compared to those that received propofol. ROS from blood also showed significant higher under anaesthesia with isoflurane. However, these findings were not associated with any changes in cytokines in blood or immunohistochemical assay in tissues. Ivyspring International Publisher 2019-09-07 /pmc/articles/PMC6775274/ /pubmed/31588195 http://dx.doi.org/10.7150/ijms.36265 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Seo, Eun-Hye Piao, Liyun Park, Hyun-Jun Lee, Ji Yeon Sa, Mijung Oh, Chung-Sik Lee, Seung-Hyun Kim, Seong-Hyop Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane |
title | Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane |
title_full | Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane |
title_fullStr | Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane |
title_full_unstemmed | Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane |
title_short | Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane |
title_sort | impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775274/ https://www.ncbi.nlm.nih.gov/pubmed/31588195 http://dx.doi.org/10.7150/ijms.36265 |
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