DNA methylation biomarkers in stool for early screening of colorectal cancer

Objective: Detection of aberrant methylated genes in feces has been developed as an early screening method for colorectal cancer. The aim of this study was to probe the methylation status of SEPT9, BMP3, NDRG4, and SDC2 in stool and study whether methylation of these genes is associated with colorec...

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Autores principales: Chen, Jie, Sun, Haipeng, Tang, Weisen, Zhou, Lin, Xie, Xi, Qu, Zhan, Chen, Mengfei, Wang, Shunyao, Yang, Ting, Dai, Ying, Wang, Yongli, Gao, Tangjie, Zhou, Qiao, Song, Zhuo, Liao, Mingmei, Liu, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2019
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775613/
https://www.ncbi.nlm.nih.gov/pubmed/31602277
http://dx.doi.org/10.7150/jca.34944
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author Chen, Jie
Sun, Haipeng
Tang, Weisen
Zhou, Lin
Xie, Xi
Qu, Zhan
Chen, Mengfei
Wang, Shunyao
Yang, Ting
Dai, Ying
Wang, Yongli
Gao, Tangjie
Zhou, Qiao
Song, Zhuo
Liao, Mingmei
Liu, Weidong
author_facet Chen, Jie
Sun, Haipeng
Tang, Weisen
Zhou, Lin
Xie, Xi
Qu, Zhan
Chen, Mengfei
Wang, Shunyao
Yang, Ting
Dai, Ying
Wang, Yongli
Gao, Tangjie
Zhou, Qiao
Song, Zhuo
Liao, Mingmei
Liu, Weidong
author_sort Chen, Jie
collection PubMed
description Objective: Detection of aberrant methylated genes in feces has been developed as an early screening method for colorectal cancer. The aim of this study was to probe the methylation status of SEPT9, BMP3, NDRG4, and SDC2 in stool and study whether methylation of these genes is associated with colorectal cancer. Materials and Methods: DNAs were isolated and purified from cancerous and non-cancerous stool samples and colorectal cancer tissue. Gene methylation levels were quantified by methylation-specific PCR on SEPT9, BMP3, NDRG4, and SDC2 and analyzed by a diagnostic model. Results: DNA methylation of SEPT9, NDRG4 and SDC2, but not BMP3, had diagnostic potential for detecting colorectal cancer. Moreover, integration of SEPT9, NDRG4, and SDC2 methylation demonstrated high feasibility for detecting colorectal cancer and adenoma, with better performance on colorectal cancer than adenoma. Conclusion: The methylation of SEPT9, NDRG4, and SDC2 in stool may be a potential biomarker for early screening of colorectal cancer.
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spelling pubmed-67756132019-10-10 DNA methylation biomarkers in stool for early screening of colorectal cancer Chen, Jie Sun, Haipeng Tang, Weisen Zhou, Lin Xie, Xi Qu, Zhan Chen, Mengfei Wang, Shunyao Yang, Ting Dai, Ying Wang, Yongli Gao, Tangjie Zhou, Qiao Song, Zhuo Liao, Mingmei Liu, Weidong J Cancer Research Paper Objective: Detection of aberrant methylated genes in feces has been developed as an early screening method for colorectal cancer. The aim of this study was to probe the methylation status of SEPT9, BMP3, NDRG4, and SDC2 in stool and study whether methylation of these genes is associated with colorectal cancer. Materials and Methods: DNAs were isolated and purified from cancerous and non-cancerous stool samples and colorectal cancer tissue. Gene methylation levels were quantified by methylation-specific PCR on SEPT9, BMP3, NDRG4, and SDC2 and analyzed by a diagnostic model. Results: DNA methylation of SEPT9, NDRG4 and SDC2, but not BMP3, had diagnostic potential for detecting colorectal cancer. Moreover, integration of SEPT9, NDRG4, and SDC2 methylation demonstrated high feasibility for detecting colorectal cancer and adenoma, with better performance on colorectal cancer than adenoma. Conclusion: The methylation of SEPT9, NDRG4, and SDC2 in stool may be a potential biomarker for early screening of colorectal cancer. Ivyspring International Publisher 2019-08-28 /pmc/articles/PMC6775613/ /pubmed/31602277 http://dx.doi.org/10.7150/jca.34944 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Chen, Jie
Sun, Haipeng
Tang, Weisen
Zhou, Lin
Xie, Xi
Qu, Zhan
Chen, Mengfei
Wang, Shunyao
Yang, Ting
Dai, Ying
Wang, Yongli
Gao, Tangjie
Zhou, Qiao
Song, Zhuo
Liao, Mingmei
Liu, Weidong
DNA methylation biomarkers in stool for early screening of colorectal cancer
title DNA methylation biomarkers in stool for early screening of colorectal cancer
title_full DNA methylation biomarkers in stool for early screening of colorectal cancer
title_fullStr DNA methylation biomarkers in stool for early screening of colorectal cancer
title_full_unstemmed DNA methylation biomarkers in stool for early screening of colorectal cancer
title_short DNA methylation biomarkers in stool for early screening of colorectal cancer
title_sort dna methylation biomarkers in stool for early screening of colorectal cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775613/
https://www.ncbi.nlm.nih.gov/pubmed/31602277
http://dx.doi.org/10.7150/jca.34944
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