Cargando…
Hypermethylation of BRM promoter plays oncogenic roles in development of clear cell renal cell carcinoma
Although the inactivation of BRM plays oncogenic roles in tumorigenesis, regulation mechanism is rarely studied in clear cell renal cell carcinoma (RCC). Thus, we aimed to investigate the mechanism of BRM inactivation and explore the tumor suppressing roles of BRM in the development of clear cell RC...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775622/ https://www.ncbi.nlm.nih.gov/pubmed/31602276 http://dx.doi.org/10.7150/jca.30098 |
_version_ | 1783456289298317312 |
---|---|
author | Fang, Ru Xia, Qiuyuan Sun, Jing ng, Hao Zha Liang, Yan Wang, Xiaotong Wang, Xuan Ma, Henghui Zhou, Xiaojun Cheng, Yang Rao, Qiu |
author_facet | Fang, Ru Xia, Qiuyuan Sun, Jing ng, Hao Zha Liang, Yan Wang, Xiaotong Wang, Xuan Ma, Henghui Zhou, Xiaojun Cheng, Yang Rao, Qiu |
author_sort | Fang, Ru |
collection | PubMed |
description | Although the inactivation of BRM plays oncogenic roles in tumorigenesis, regulation mechanism is rarely studied in clear cell renal cell carcinoma (RCC). Thus, we aimed to investigate the mechanism of BRM inactivation and explore the tumor suppressing roles of BRM in the development of clear cell RCC. We verified that hypermethylation of the BRM promoter was correlated with decreased expression of BRM by multi-omics analysis based on the TCGA database. This result was further confirmed in our own tumor tissues. Moreover, BRM inhibited the ability of proliferation and invasion of RCC cells in vitro. Consistent with this, BRM overexpressing virtually inhibited the xenograft tumor growth of ACHN cells in vivo. Finally we found that BRM promoted cell apoptosis and cellular cycle arrest in G2/M. In conclusion, our study confirms that the hypermethylation of BRM promoters plays oncogenic roles by the transcription inhibition of BRM in RCC, and the tumor suppressor gene BRM inhibits RCC cell vitality in vitro and in vivo. |
format | Online Article Text |
id | pubmed-6775622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-67756222019-10-10 Hypermethylation of BRM promoter plays oncogenic roles in development of clear cell renal cell carcinoma Fang, Ru Xia, Qiuyuan Sun, Jing ng, Hao Zha Liang, Yan Wang, Xiaotong Wang, Xuan Ma, Henghui Zhou, Xiaojun Cheng, Yang Rao, Qiu J Cancer Research Paper Although the inactivation of BRM plays oncogenic roles in tumorigenesis, regulation mechanism is rarely studied in clear cell renal cell carcinoma (RCC). Thus, we aimed to investigate the mechanism of BRM inactivation and explore the tumor suppressing roles of BRM in the development of clear cell RCC. We verified that hypermethylation of the BRM promoter was correlated with decreased expression of BRM by multi-omics analysis based on the TCGA database. This result was further confirmed in our own tumor tissues. Moreover, BRM inhibited the ability of proliferation and invasion of RCC cells in vitro. Consistent with this, BRM overexpressing virtually inhibited the xenograft tumor growth of ACHN cells in vivo. Finally we found that BRM promoted cell apoptosis and cellular cycle arrest in G2/M. In conclusion, our study confirms that the hypermethylation of BRM promoters plays oncogenic roles by the transcription inhibition of BRM in RCC, and the tumor suppressor gene BRM inhibits RCC cell vitality in vitro and in vivo. Ivyspring International Publisher 2019-08-28 /pmc/articles/PMC6775622/ /pubmed/31602276 http://dx.doi.org/10.7150/jca.30098 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Fang, Ru Xia, Qiuyuan Sun, Jing ng, Hao Zha Liang, Yan Wang, Xiaotong Wang, Xuan Ma, Henghui Zhou, Xiaojun Cheng, Yang Rao, Qiu Hypermethylation of BRM promoter plays oncogenic roles in development of clear cell renal cell carcinoma |
title | Hypermethylation of BRM promoter plays oncogenic roles in development of clear cell renal cell carcinoma |
title_full | Hypermethylation of BRM promoter plays oncogenic roles in development of clear cell renal cell carcinoma |
title_fullStr | Hypermethylation of BRM promoter plays oncogenic roles in development of clear cell renal cell carcinoma |
title_full_unstemmed | Hypermethylation of BRM promoter plays oncogenic roles in development of clear cell renal cell carcinoma |
title_short | Hypermethylation of BRM promoter plays oncogenic roles in development of clear cell renal cell carcinoma |
title_sort | hypermethylation of brm promoter plays oncogenic roles in development of clear cell renal cell carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6775622/ https://www.ncbi.nlm.nih.gov/pubmed/31602276 http://dx.doi.org/10.7150/jca.30098 |
work_keys_str_mv | AT fangru hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma AT xiaqiuyuan hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma AT sunjing hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma AT nghaozha hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma AT liangyan hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma AT wangxiaotong hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma AT wangxuan hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma AT mahenghui hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma AT zhouxiaojun hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma AT chengyang hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma AT raoqiu hypermethylationofbrmpromoterplaysoncogenicrolesindevelopmentofclearcellrenalcellcarcinoma |