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Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH

Enhanced biological phosphorus removal (EBPR) is a globally important biotechnological process and relies on the massive accumulation of phosphate within special microorganisms. Candidatus Accumulibacter conform to the classical physiology model for polyphosphate accumulating organisms and are widel...

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Autores principales: Fernando, Eustace Y., McIlroy, Simon Jon, Nierychlo, Marta, Herbst, Florian-Alexander, Petriglieri, Francesca, Schmid, Markus C., Wagner, Michael, Nielsen, Jeppe Lund, Nielsen, Per Halkjær
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776032/
https://www.ncbi.nlm.nih.gov/pubmed/30894691
http://dx.doi.org/10.1038/s41396-019-0399-7
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author Fernando, Eustace Y.
McIlroy, Simon Jon
Nierychlo, Marta
Herbst, Florian-Alexander
Petriglieri, Francesca
Schmid, Markus C.
Wagner, Michael
Nielsen, Jeppe Lund
Nielsen, Per Halkjær
author_facet Fernando, Eustace Y.
McIlroy, Simon Jon
Nierychlo, Marta
Herbst, Florian-Alexander
Petriglieri, Francesca
Schmid, Markus C.
Wagner, Michael
Nielsen, Jeppe Lund
Nielsen, Per Halkjær
author_sort Fernando, Eustace Y.
collection PubMed
description Enhanced biological phosphorus removal (EBPR) is a globally important biotechnological process and relies on the massive accumulation of phosphate within special microorganisms. Candidatus Accumulibacter conform to the classical physiology model for polyphosphate accumulating organisms and are widely believed to be the most important player for the process in full-scale EBPR systems. However, it was impossible till now to quantify the contribution of specific microbial clades to EBPR. In this study, we have developed a new tool to directly link the identity of microbial cells to the absolute quantification of intracellular poly-P and other polymers under in situ conditions, and applied it to eight full-scale EBPR plants. Besides Ca. Accumulibacter, members of the genus Tetrasphaera were found to be important microbes for P accumulation, and in six plants they were the most important. As these Tetrasphaera cells did not exhibit the classical phenotype of poly-P accumulating microbes, our entire understanding of the microbiology of the EBPR process has to be revised. Furthermore, our new single-cell approach can now also be applied to quantify storage polymer dynamics in individual populations in situ in other ecosystems and might become a valuable tool for many environmental microbiologists.
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spelling pubmed-67760322019-10-04 Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH Fernando, Eustace Y. McIlroy, Simon Jon Nierychlo, Marta Herbst, Florian-Alexander Petriglieri, Francesca Schmid, Markus C. Wagner, Michael Nielsen, Jeppe Lund Nielsen, Per Halkjær ISME J Article Enhanced biological phosphorus removal (EBPR) is a globally important biotechnological process and relies on the massive accumulation of phosphate within special microorganisms. Candidatus Accumulibacter conform to the classical physiology model for polyphosphate accumulating organisms and are widely believed to be the most important player for the process in full-scale EBPR systems. However, it was impossible till now to quantify the contribution of specific microbial clades to EBPR. In this study, we have developed a new tool to directly link the identity of microbial cells to the absolute quantification of intracellular poly-P and other polymers under in situ conditions, and applied it to eight full-scale EBPR plants. Besides Ca. Accumulibacter, members of the genus Tetrasphaera were found to be important microbes for P accumulation, and in six plants they were the most important. As these Tetrasphaera cells did not exhibit the classical phenotype of poly-P accumulating microbes, our entire understanding of the microbiology of the EBPR process has to be revised. Furthermore, our new single-cell approach can now also be applied to quantify storage polymer dynamics in individual populations in situ in other ecosystems and might become a valuable tool for many environmental microbiologists. Nature Publishing Group UK 2019-03-20 2019-08 /pmc/articles/PMC6776032/ /pubmed/30894691 http://dx.doi.org/10.1038/s41396-019-0399-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fernando, Eustace Y.
McIlroy, Simon Jon
Nierychlo, Marta
Herbst, Florian-Alexander
Petriglieri, Francesca
Schmid, Markus C.
Wagner, Michael
Nielsen, Jeppe Lund
Nielsen, Per Halkjær
Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH
title Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH
title_full Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH
title_fullStr Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH
title_full_unstemmed Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH
title_short Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH
title_sort resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with raman–fish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776032/
https://www.ncbi.nlm.nih.gov/pubmed/30894691
http://dx.doi.org/10.1038/s41396-019-0399-7
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