Cargando…

Alternative Oxidase (AOX) Senses Stress Levels to Coordinate Auxin-Induced Reprogramming From Seed Germination to Somatic Embryogenesis—A Role Relevant for Seed Vigor Prediction and Plant Robustness

Somatic embryogenesis (SE) is the most striking and prominent example of plant plasticity upon severe stress. Inducing immature carrot seeds perform SE as substitute to germination by auxin treatment can be seen as switch between stress levels associated to morphophysiological plasticity. This exper...

Descripción completa

Detalles Bibliográficos
Autores principales: Mohanapriya, Gunasekaran, Bharadwaj, Revuru, Noceda, Carlos, Costa, José Hélio, Kumar, Sarma Rajeev, Sathishkumar, Ramalingam, Thiers, Karine Leitão Lima, Santos Macedo, Elisete, Silva, Sofia, Annicchiarico, Paolo, Groot, Steven P.C., Kodde, Jan, Kumari, Aprajita, Gupta, Kapuganti Jagadis, Arnholdt-Schmitt, Birgit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776121/
https://www.ncbi.nlm.nih.gov/pubmed/31611888
http://dx.doi.org/10.3389/fpls.2019.01134
_version_ 1783456366010040320
author Mohanapriya, Gunasekaran
Bharadwaj, Revuru
Noceda, Carlos
Costa, José Hélio
Kumar, Sarma Rajeev
Sathishkumar, Ramalingam
Thiers, Karine Leitão Lima
Santos Macedo, Elisete
Silva, Sofia
Annicchiarico, Paolo
Groot, Steven P.C.
Kodde, Jan
Kumari, Aprajita
Gupta, Kapuganti Jagadis
Arnholdt-Schmitt, Birgit
author_facet Mohanapriya, Gunasekaran
Bharadwaj, Revuru
Noceda, Carlos
Costa, José Hélio
Kumar, Sarma Rajeev
Sathishkumar, Ramalingam
Thiers, Karine Leitão Lima
Santos Macedo, Elisete
Silva, Sofia
Annicchiarico, Paolo
Groot, Steven P.C.
Kodde, Jan
Kumari, Aprajita
Gupta, Kapuganti Jagadis
Arnholdt-Schmitt, Birgit
author_sort Mohanapriya, Gunasekaran
collection PubMed
description Somatic embryogenesis (SE) is the most striking and prominent example of plant plasticity upon severe stress. Inducing immature carrot seeds perform SE as substitute to germination by auxin treatment can be seen as switch between stress levels associated to morphophysiological plasticity. This experimental system is highly powerful to explore stress response factors that mediate the metabolic switch between cell and tissue identities. Developmental plasticity per se is an emerging trait for in vitro systems and crop improvement. It is supposed to underlie multi-stress tolerance. High plasticity can protect plants throughout life cycles against variable abiotic and biotic conditions. We provide proof of concepts for the existing hypothesis that alternative oxidase (AOX) can be relevant for developmental plasticity and be associated to yield stability. Our perspective on AOX as relevant coordinator of cell reprogramming is supported by real-time polymerase chain reaction (PCR) analyses and gross metabolism data from calorespirometry complemented by SHAM-inhibitor studies on primed, elevated partial pressure of oxygen (EPPO)–stressed, and endophyte-treated seeds. In silico studies on public experimental data from diverse species strengthen generality of our insights. Finally, we highlight ready-to-use concepts for plant selection and optimizing in vivo and in vitro propagation that do not require further details on molecular physiology and metabolism. This is demonstrated by applying our research & technology concepts to pea genotypes with differential yield performance in multilocation fields and chickpea types known for differential robustness in the field. By using these concepts and tools appropriately, also other marker candidates than AOX and complex genomics data can be efficiently validated for prebreeding and seed vigor prediction.
format Online
Article
Text
id pubmed-6776121
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-67761212019-10-14 Alternative Oxidase (AOX) Senses Stress Levels to Coordinate Auxin-Induced Reprogramming From Seed Germination to Somatic Embryogenesis—A Role Relevant for Seed Vigor Prediction and Plant Robustness Mohanapriya, Gunasekaran Bharadwaj, Revuru Noceda, Carlos Costa, José Hélio Kumar, Sarma Rajeev Sathishkumar, Ramalingam Thiers, Karine Leitão Lima Santos Macedo, Elisete Silva, Sofia Annicchiarico, Paolo Groot, Steven P.C. Kodde, Jan Kumari, Aprajita Gupta, Kapuganti Jagadis Arnholdt-Schmitt, Birgit Front Plant Sci Plant Science Somatic embryogenesis (SE) is the most striking and prominent example of plant plasticity upon severe stress. Inducing immature carrot seeds perform SE as substitute to germination by auxin treatment can be seen as switch between stress levels associated to morphophysiological plasticity. This experimental system is highly powerful to explore stress response factors that mediate the metabolic switch between cell and tissue identities. Developmental plasticity per se is an emerging trait for in vitro systems and crop improvement. It is supposed to underlie multi-stress tolerance. High plasticity can protect plants throughout life cycles against variable abiotic and biotic conditions. We provide proof of concepts for the existing hypothesis that alternative oxidase (AOX) can be relevant for developmental plasticity and be associated to yield stability. Our perspective on AOX as relevant coordinator of cell reprogramming is supported by real-time polymerase chain reaction (PCR) analyses and gross metabolism data from calorespirometry complemented by SHAM-inhibitor studies on primed, elevated partial pressure of oxygen (EPPO)–stressed, and endophyte-treated seeds. In silico studies on public experimental data from diverse species strengthen generality of our insights. Finally, we highlight ready-to-use concepts for plant selection and optimizing in vivo and in vitro propagation that do not require further details on molecular physiology and metabolism. This is demonstrated by applying our research & technology concepts to pea genotypes with differential yield performance in multilocation fields and chickpea types known for differential robustness in the field. By using these concepts and tools appropriately, also other marker candidates than AOX and complex genomics data can be efficiently validated for prebreeding and seed vigor prediction. Frontiers Media S.A. 2019-09-20 /pmc/articles/PMC6776121/ /pubmed/31611888 http://dx.doi.org/10.3389/fpls.2019.01134 Text en Copyright © 2019 Mohanapriya, Bharadwaj, Noceda, Costa, Kumar, Sathishkumar, Thiers, Santos Macedo, Silva, Annicchiarico, Groot, Kodde, Kumari, Gupta and Arnholdt-Schmitt http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Mohanapriya, Gunasekaran
Bharadwaj, Revuru
Noceda, Carlos
Costa, José Hélio
Kumar, Sarma Rajeev
Sathishkumar, Ramalingam
Thiers, Karine Leitão Lima
Santos Macedo, Elisete
Silva, Sofia
Annicchiarico, Paolo
Groot, Steven P.C.
Kodde, Jan
Kumari, Aprajita
Gupta, Kapuganti Jagadis
Arnholdt-Schmitt, Birgit
Alternative Oxidase (AOX) Senses Stress Levels to Coordinate Auxin-Induced Reprogramming From Seed Germination to Somatic Embryogenesis—A Role Relevant for Seed Vigor Prediction and Plant Robustness
title Alternative Oxidase (AOX) Senses Stress Levels to Coordinate Auxin-Induced Reprogramming From Seed Germination to Somatic Embryogenesis—A Role Relevant for Seed Vigor Prediction and Plant Robustness
title_full Alternative Oxidase (AOX) Senses Stress Levels to Coordinate Auxin-Induced Reprogramming From Seed Germination to Somatic Embryogenesis—A Role Relevant for Seed Vigor Prediction and Plant Robustness
title_fullStr Alternative Oxidase (AOX) Senses Stress Levels to Coordinate Auxin-Induced Reprogramming From Seed Germination to Somatic Embryogenesis—A Role Relevant for Seed Vigor Prediction and Plant Robustness
title_full_unstemmed Alternative Oxidase (AOX) Senses Stress Levels to Coordinate Auxin-Induced Reprogramming From Seed Germination to Somatic Embryogenesis—A Role Relevant for Seed Vigor Prediction and Plant Robustness
title_short Alternative Oxidase (AOX) Senses Stress Levels to Coordinate Auxin-Induced Reprogramming From Seed Germination to Somatic Embryogenesis—A Role Relevant for Seed Vigor Prediction and Plant Robustness
title_sort alternative oxidase (aox) senses stress levels to coordinate auxin-induced reprogramming from seed germination to somatic embryogenesis—a role relevant for seed vigor prediction and plant robustness
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776121/
https://www.ncbi.nlm.nih.gov/pubmed/31611888
http://dx.doi.org/10.3389/fpls.2019.01134
work_keys_str_mv AT mohanapriyagunasekaran alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT bharadwajrevuru alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT nocedacarlos alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT costajosehelio alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT kumarsarmarajeev alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT sathishkumarramalingam alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT thierskarineleitaolima alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT santosmacedoelisete alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT silvasofia alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT annicchiaricopaolo alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT grootstevenpc alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT koddejan alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT kumariaprajita alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT guptakapugantijagadis alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness
AT arnholdtschmittbirgit alternativeoxidaseaoxsensesstresslevelstocoordinateauxininducedreprogrammingfromseedgerminationtosomaticembryogenesisarolerelevantforseedvigorpredictionandplantrobustness