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Field assessment of a major QTL associated with tolerance to cold-induced seed coat discoloration in soybean

In Hokkaido, the northernmost region of Japan, soybean [Glycine max (L.) Merr.] crops are damaged by cold weather. Chilling temperatures negatively affect seed appearance by causing seed coat discoloration around the hilum region, which is called cold-induced discoloration (CD). An assay for CD tole...

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Autores principales: Yamaguchi, Naoya, Hagihara, Seiji, Hirai, Dai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776143/
https://www.ncbi.nlm.nih.gov/pubmed/31598087
http://dx.doi.org/10.1270/jsbbs.19024
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author Yamaguchi, Naoya
Hagihara, Seiji
Hirai, Dai
author_facet Yamaguchi, Naoya
Hagihara, Seiji
Hirai, Dai
author_sort Yamaguchi, Naoya
collection PubMed
description In Hokkaido, the northernmost region of Japan, soybean [Glycine max (L.) Merr.] crops are damaged by cold weather. Chilling temperatures negatively affect seed appearance by causing seed coat discoloration around the hilum region, which is called cold-induced discoloration (CD). An assay for CD tolerance using a phytotron was developed, and two quantitative trait loci (QTLs) associated with CD tolerance were identified. The major QTL was located in the proximal region of the I locus, and structural variation of this locus can serve as a useful DNA marker, called the Ic marker. To use this marker in breeding programs, the effects need to be assessed under field conditions because the Ic marker has been developed solely under phytotron conditions. The aim of this study was thus to assess the effect of the Ic marker under a cool field environment. We confirmed that the Ic allele was highly effective using 27 cultivars and breeding lines including a near-isogenic line grown in the field where severe cold-weather damage occurred. This allele had no negative influence on the agronomic traits in the near-isogenic line. Our results suggest that marker-assisted selection for the Ic allele is effective for improving CD tolerance in breeding programs.
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spelling pubmed-67761432019-10-09 Field assessment of a major QTL associated with tolerance to cold-induced seed coat discoloration in soybean Yamaguchi, Naoya Hagihara, Seiji Hirai, Dai Breed Sci Note In Hokkaido, the northernmost region of Japan, soybean [Glycine max (L.) Merr.] crops are damaged by cold weather. Chilling temperatures negatively affect seed appearance by causing seed coat discoloration around the hilum region, which is called cold-induced discoloration (CD). An assay for CD tolerance using a phytotron was developed, and two quantitative trait loci (QTLs) associated with CD tolerance were identified. The major QTL was located in the proximal region of the I locus, and structural variation of this locus can serve as a useful DNA marker, called the Ic marker. To use this marker in breeding programs, the effects need to be assessed under field conditions because the Ic marker has been developed solely under phytotron conditions. The aim of this study was thus to assess the effect of the Ic marker under a cool field environment. We confirmed that the Ic allele was highly effective using 27 cultivars and breeding lines including a near-isogenic line grown in the field where severe cold-weather damage occurred. This allele had no negative influence on the agronomic traits in the near-isogenic line. Our results suggest that marker-assisted selection for the Ic allele is effective for improving CD tolerance in breeding programs. Japanese Society of Breeding 2019-09 2019-07-19 /pmc/articles/PMC6776143/ /pubmed/31598087 http://dx.doi.org/10.1270/jsbbs.19024 Text en Copyright © 2019 by JAPANESE SOCIETY OF BREEDING http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Yamaguchi, Naoya
Hagihara, Seiji
Hirai, Dai
Field assessment of a major QTL associated with tolerance to cold-induced seed coat discoloration in soybean
title Field assessment of a major QTL associated with tolerance to cold-induced seed coat discoloration in soybean
title_full Field assessment of a major QTL associated with tolerance to cold-induced seed coat discoloration in soybean
title_fullStr Field assessment of a major QTL associated with tolerance to cold-induced seed coat discoloration in soybean
title_full_unstemmed Field assessment of a major QTL associated with tolerance to cold-induced seed coat discoloration in soybean
title_short Field assessment of a major QTL associated with tolerance to cold-induced seed coat discoloration in soybean
title_sort field assessment of a major qtl associated with tolerance to cold-induced seed coat discoloration in soybean
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776143/
https://www.ncbi.nlm.nih.gov/pubmed/31598087
http://dx.doi.org/10.1270/jsbbs.19024
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