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Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing

Lutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations hel...

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Detalles Bibliográficos
Autores principales: Casaril, Aline Etelvina, Alonso, Diego Peres, Franco, Karina Garcia, Alvarez, Marcus Vinicius Niz, Barrios, Suellem Petilim Gomes, Fernandes, Wagner de Souza, Infran, Jucelei de Oliveira Moura, Rodrigues, Ana Caroline Moura, Ribolla, Paulo Eduardo Martins, de Oliveira, Alessandra Gutierrez
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776309/
https://www.ncbi.nlm.nih.gov/pubmed/31581227
http://dx.doi.org/10.1371/journal.pone.0223277
Descripción
Sumario:Lutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations helps the elucidation of components and interactions of the disease ecoepidemiology. Thus, the objective of this study was to genotypically analyze populations of the Lu. longipalpis complex from a macrogeographic perspective using Next Generation Sequencing. Polymorphism analysis of three molecular markers was used to access the levels of population genetic structure among nine different populations of sand flies. Illumina Amplicon Sequencing Protocol(®) was used to identify possible polymorphic sites. The library was sequenced on paired-end Illumina MiSeq platform. Significant macrogeographical population differentiation was observed among Lu. longipalpis populations via PCA and DAPC analyses. Our results revealed that populations of Lu. longipalpis from the nine municipalities were grouped into three clusters. In addition, it was observed that the levels of Lu. longipalpis population structure could be associated with distance isolation. This new sequencing method allowed us to study different molecular markers after a single sequencing run, and to evaluate population and inter-species differences on a macrogeographic scale.