Cargando…

Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing

Lutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations hel...

Descripción completa

Detalles Bibliográficos
Autores principales: Casaril, Aline Etelvina, Alonso, Diego Peres, Franco, Karina Garcia, Alvarez, Marcus Vinicius Niz, Barrios, Suellem Petilim Gomes, Fernandes, Wagner de Souza, Infran, Jucelei de Oliveira Moura, Rodrigues, Ana Caroline Moura, Ribolla, Paulo Eduardo Martins, de Oliveira, Alessandra Gutierrez
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776309/
https://www.ncbi.nlm.nih.gov/pubmed/31581227
http://dx.doi.org/10.1371/journal.pone.0223277
_version_ 1783456396567642112
author Casaril, Aline Etelvina
Alonso, Diego Peres
Franco, Karina Garcia
Alvarez, Marcus Vinicius Niz
Barrios, Suellem Petilim Gomes
Fernandes, Wagner de Souza
Infran, Jucelei de Oliveira Moura
Rodrigues, Ana Caroline Moura
Ribolla, Paulo Eduardo Martins
de Oliveira, Alessandra Gutierrez
author_facet Casaril, Aline Etelvina
Alonso, Diego Peres
Franco, Karina Garcia
Alvarez, Marcus Vinicius Niz
Barrios, Suellem Petilim Gomes
Fernandes, Wagner de Souza
Infran, Jucelei de Oliveira Moura
Rodrigues, Ana Caroline Moura
Ribolla, Paulo Eduardo Martins
de Oliveira, Alessandra Gutierrez
author_sort Casaril, Aline Etelvina
collection PubMed
description Lutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations helps the elucidation of components and interactions of the disease ecoepidemiology. Thus, the objective of this study was to genotypically analyze populations of the Lu. longipalpis complex from a macrogeographic perspective using Next Generation Sequencing. Polymorphism analysis of three molecular markers was used to access the levels of population genetic structure among nine different populations of sand flies. Illumina Amplicon Sequencing Protocol(®) was used to identify possible polymorphic sites. The library was sequenced on paired-end Illumina MiSeq platform. Significant macrogeographical population differentiation was observed among Lu. longipalpis populations via PCA and DAPC analyses. Our results revealed that populations of Lu. longipalpis from the nine municipalities were grouped into three clusters. In addition, it was observed that the levels of Lu. longipalpis population structure could be associated with distance isolation. This new sequencing method allowed us to study different molecular markers after a single sequencing run, and to evaluate population and inter-species differences on a macrogeographic scale.
format Online
Article
Text
id pubmed-6776309
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-67763092019-10-12 Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing Casaril, Aline Etelvina Alonso, Diego Peres Franco, Karina Garcia Alvarez, Marcus Vinicius Niz Barrios, Suellem Petilim Gomes Fernandes, Wagner de Souza Infran, Jucelei de Oliveira Moura Rodrigues, Ana Caroline Moura Ribolla, Paulo Eduardo Martins de Oliveira, Alessandra Gutierrez PLoS One Research Article Lutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations helps the elucidation of components and interactions of the disease ecoepidemiology. Thus, the objective of this study was to genotypically analyze populations of the Lu. longipalpis complex from a macrogeographic perspective using Next Generation Sequencing. Polymorphism analysis of three molecular markers was used to access the levels of population genetic structure among nine different populations of sand flies. Illumina Amplicon Sequencing Protocol(®) was used to identify possible polymorphic sites. The library was sequenced on paired-end Illumina MiSeq platform. Significant macrogeographical population differentiation was observed among Lu. longipalpis populations via PCA and DAPC analyses. Our results revealed that populations of Lu. longipalpis from the nine municipalities were grouped into three clusters. In addition, it was observed that the levels of Lu. longipalpis population structure could be associated with distance isolation. This new sequencing method allowed us to study different molecular markers after a single sequencing run, and to evaluate population and inter-species differences on a macrogeographic scale. Public Library of Science 2019-10-03 /pmc/articles/PMC6776309/ /pubmed/31581227 http://dx.doi.org/10.1371/journal.pone.0223277 Text en © 2019 Casaril et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Casaril, Aline Etelvina
Alonso, Diego Peres
Franco, Karina Garcia
Alvarez, Marcus Vinicius Niz
Barrios, Suellem Petilim Gomes
Fernandes, Wagner de Souza
Infran, Jucelei de Oliveira Moura
Rodrigues, Ana Caroline Moura
Ribolla, Paulo Eduardo Martins
de Oliveira, Alessandra Gutierrez
Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing
title Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing
title_full Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing
title_fullStr Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing
title_full_unstemmed Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing
title_short Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing
title_sort macrogeographic genetic structure of lutzomyia longipalpis complex populations using next generation sequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776309/
https://www.ncbi.nlm.nih.gov/pubmed/31581227
http://dx.doi.org/10.1371/journal.pone.0223277
work_keys_str_mv AT casarilalineetelvina macrogeographicgeneticstructureoflutzomyialongipalpiscomplexpopulationsusingnextgenerationsequencing
AT alonsodiegoperes macrogeographicgeneticstructureoflutzomyialongipalpiscomplexpopulationsusingnextgenerationsequencing
AT francokarinagarcia macrogeographicgeneticstructureoflutzomyialongipalpiscomplexpopulationsusingnextgenerationsequencing
AT alvarezmarcusviniciusniz macrogeographicgeneticstructureoflutzomyialongipalpiscomplexpopulationsusingnextgenerationsequencing
AT barriossuellempetilimgomes macrogeographicgeneticstructureoflutzomyialongipalpiscomplexpopulationsusingnextgenerationsequencing
AT fernandeswagnerdesouza macrogeographicgeneticstructureoflutzomyialongipalpiscomplexpopulationsusingnextgenerationsequencing
AT infranjuceleideoliveiramoura macrogeographicgeneticstructureoflutzomyialongipalpiscomplexpopulationsusingnextgenerationsequencing
AT rodriguesanacarolinemoura macrogeographicgeneticstructureoflutzomyialongipalpiscomplexpopulationsusingnextgenerationsequencing
AT ribollapauloeduardomartins macrogeographicgeneticstructureoflutzomyialongipalpiscomplexpopulationsusingnextgenerationsequencing
AT deoliveiraalessandragutierrez macrogeographicgeneticstructureoflutzomyialongipalpiscomplexpopulationsusingnextgenerationsequencing