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Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing
Lutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations hel...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776309/ https://www.ncbi.nlm.nih.gov/pubmed/31581227 http://dx.doi.org/10.1371/journal.pone.0223277 |
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author | Casaril, Aline Etelvina Alonso, Diego Peres Franco, Karina Garcia Alvarez, Marcus Vinicius Niz Barrios, Suellem Petilim Gomes Fernandes, Wagner de Souza Infran, Jucelei de Oliveira Moura Rodrigues, Ana Caroline Moura Ribolla, Paulo Eduardo Martins de Oliveira, Alessandra Gutierrez |
author_facet | Casaril, Aline Etelvina Alonso, Diego Peres Franco, Karina Garcia Alvarez, Marcus Vinicius Niz Barrios, Suellem Petilim Gomes Fernandes, Wagner de Souza Infran, Jucelei de Oliveira Moura Rodrigues, Ana Caroline Moura Ribolla, Paulo Eduardo Martins de Oliveira, Alessandra Gutierrez |
author_sort | Casaril, Aline Etelvina |
collection | PubMed |
description | Lutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations helps the elucidation of components and interactions of the disease ecoepidemiology. Thus, the objective of this study was to genotypically analyze populations of the Lu. longipalpis complex from a macrogeographic perspective using Next Generation Sequencing. Polymorphism analysis of three molecular markers was used to access the levels of population genetic structure among nine different populations of sand flies. Illumina Amplicon Sequencing Protocol(®) was used to identify possible polymorphic sites. The library was sequenced on paired-end Illumina MiSeq platform. Significant macrogeographical population differentiation was observed among Lu. longipalpis populations via PCA and DAPC analyses. Our results revealed that populations of Lu. longipalpis from the nine municipalities were grouped into three clusters. In addition, it was observed that the levels of Lu. longipalpis population structure could be associated with distance isolation. This new sequencing method allowed us to study different molecular markers after a single sequencing run, and to evaluate population and inter-species differences on a macrogeographic scale. |
format | Online Article Text |
id | pubmed-6776309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-67763092019-10-12 Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing Casaril, Aline Etelvina Alonso, Diego Peres Franco, Karina Garcia Alvarez, Marcus Vinicius Niz Barrios, Suellem Petilim Gomes Fernandes, Wagner de Souza Infran, Jucelei de Oliveira Moura Rodrigues, Ana Caroline Moura Ribolla, Paulo Eduardo Martins de Oliveira, Alessandra Gutierrez PLoS One Research Article Lutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations helps the elucidation of components and interactions of the disease ecoepidemiology. Thus, the objective of this study was to genotypically analyze populations of the Lu. longipalpis complex from a macrogeographic perspective using Next Generation Sequencing. Polymorphism analysis of three molecular markers was used to access the levels of population genetic structure among nine different populations of sand flies. Illumina Amplicon Sequencing Protocol(®) was used to identify possible polymorphic sites. The library was sequenced on paired-end Illumina MiSeq platform. Significant macrogeographical population differentiation was observed among Lu. longipalpis populations via PCA and DAPC analyses. Our results revealed that populations of Lu. longipalpis from the nine municipalities were grouped into three clusters. In addition, it was observed that the levels of Lu. longipalpis population structure could be associated with distance isolation. This new sequencing method allowed us to study different molecular markers after a single sequencing run, and to evaluate population and inter-species differences on a macrogeographic scale. Public Library of Science 2019-10-03 /pmc/articles/PMC6776309/ /pubmed/31581227 http://dx.doi.org/10.1371/journal.pone.0223277 Text en © 2019 Casaril et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Casaril, Aline Etelvina Alonso, Diego Peres Franco, Karina Garcia Alvarez, Marcus Vinicius Niz Barrios, Suellem Petilim Gomes Fernandes, Wagner de Souza Infran, Jucelei de Oliveira Moura Rodrigues, Ana Caroline Moura Ribolla, Paulo Eduardo Martins de Oliveira, Alessandra Gutierrez Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title | Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title_full | Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title_fullStr | Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title_full_unstemmed | Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title_short | Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title_sort | macrogeographic genetic structure of lutzomyia longipalpis complex populations using next generation sequencing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776309/ https://www.ncbi.nlm.nih.gov/pubmed/31581227 http://dx.doi.org/10.1371/journal.pone.0223277 |
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