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Artificial fingerprints for cross-comparison of forensic DNA and protein recovery methods
Quantitative genomic and proteomic evaluation of human latent fingerprint depositions represents a challenge within the forensic field, due to the high variability in the amount of DNA and protein initially deposited. To better assess recovery techniques for touch depositions, we present a method to...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776342/ https://www.ncbi.nlm.nih.gov/pubmed/31581206 http://dx.doi.org/10.1371/journal.pone.0223170 |
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author | LeSassier, Danielle S. Schulte, Kathleen Q. Manley, Tara E. Smith, Alan R. Powals, Megan L. Albright, Nicolette C. Ludolph, Benjamin C. Weber, Katharina L. Woerner, August E. Gardner, Myles W. Hewitt, F. Curtis |
author_facet | LeSassier, Danielle S. Schulte, Kathleen Q. Manley, Tara E. Smith, Alan R. Powals, Megan L. Albright, Nicolette C. Ludolph, Benjamin C. Weber, Katharina L. Woerner, August E. Gardner, Myles W. Hewitt, F. Curtis |
author_sort | LeSassier, Danielle S. |
collection | PubMed |
description | Quantitative genomic and proteomic evaluation of human latent fingerprint depositions represents a challenge within the forensic field, due to the high variability in the amount of DNA and protein initially deposited. To better assess recovery techniques for touch depositions, we present a method to produce simple and customizable artificial fingerprints. These artificial fingerprint samples include the primary components of a typical latent fingerprint, specifically sebaceous fluid, eccrine perspiration, extracellular DNA, and proteinaceous epidermal skin material (i.e., shed skin cells). A commercially available emulsion of sebaceous and eccrine perspiration material provides a chemically-relevant suspension solution for fingerprint deposition, simplifying artificial fingerprint production. Extracted human genomic DNA is added to accurately mimic the extracellular DNA content of a typical latent print and comparable DNA yields are recovered from the artificial prints relative to human prints across surface types. Capitalizing on recent advancements in the use of protein sequence identification for human forensic analysis, these samples also contain a representative quantity of protein, originating from epidermal skin cells collected from the fingers and palms of volunteers. Proteomic sequencing by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicates a high level of protein overlap between artificial and latent prints. Data are available via ProteomeXchange with identifier PXD015445. By including known quantities of DNA and protein into each artificial print, this method enables total DNA and protein recovery to be quantitatively assessed across different sample collection and extraction methods to better evaluate extraction efficiency. Collectively, these artificial fingerprint samples are simple to make, highly versatile and customizable, and accurately represent the biochemical composition and biological signatures of human fingerprints. |
format | Online Article Text |
id | pubmed-6776342 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-67763422019-10-12 Artificial fingerprints for cross-comparison of forensic DNA and protein recovery methods LeSassier, Danielle S. Schulte, Kathleen Q. Manley, Tara E. Smith, Alan R. Powals, Megan L. Albright, Nicolette C. Ludolph, Benjamin C. Weber, Katharina L. Woerner, August E. Gardner, Myles W. Hewitt, F. Curtis PLoS One Research Article Quantitative genomic and proteomic evaluation of human latent fingerprint depositions represents a challenge within the forensic field, due to the high variability in the amount of DNA and protein initially deposited. To better assess recovery techniques for touch depositions, we present a method to produce simple and customizable artificial fingerprints. These artificial fingerprint samples include the primary components of a typical latent fingerprint, specifically sebaceous fluid, eccrine perspiration, extracellular DNA, and proteinaceous epidermal skin material (i.e., shed skin cells). A commercially available emulsion of sebaceous and eccrine perspiration material provides a chemically-relevant suspension solution for fingerprint deposition, simplifying artificial fingerprint production. Extracted human genomic DNA is added to accurately mimic the extracellular DNA content of a typical latent print and comparable DNA yields are recovered from the artificial prints relative to human prints across surface types. Capitalizing on recent advancements in the use of protein sequence identification for human forensic analysis, these samples also contain a representative quantity of protein, originating from epidermal skin cells collected from the fingers and palms of volunteers. Proteomic sequencing by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicates a high level of protein overlap between artificial and latent prints. Data are available via ProteomeXchange with identifier PXD015445. By including known quantities of DNA and protein into each artificial print, this method enables total DNA and protein recovery to be quantitatively assessed across different sample collection and extraction methods to better evaluate extraction efficiency. Collectively, these artificial fingerprint samples are simple to make, highly versatile and customizable, and accurately represent the biochemical composition and biological signatures of human fingerprints. Public Library of Science 2019-10-03 /pmc/articles/PMC6776342/ /pubmed/31581206 http://dx.doi.org/10.1371/journal.pone.0223170 Text en © 2019 LeSassier et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article LeSassier, Danielle S. Schulte, Kathleen Q. Manley, Tara E. Smith, Alan R. Powals, Megan L. Albright, Nicolette C. Ludolph, Benjamin C. Weber, Katharina L. Woerner, August E. Gardner, Myles W. Hewitt, F. Curtis Artificial fingerprints for cross-comparison of forensic DNA and protein recovery methods |
title | Artificial fingerprints for cross-comparison of forensic DNA and protein recovery methods |
title_full | Artificial fingerprints for cross-comparison of forensic DNA and protein recovery methods |
title_fullStr | Artificial fingerprints for cross-comparison of forensic DNA and protein recovery methods |
title_full_unstemmed | Artificial fingerprints for cross-comparison of forensic DNA and protein recovery methods |
title_short | Artificial fingerprints for cross-comparison of forensic DNA and protein recovery methods |
title_sort | artificial fingerprints for cross-comparison of forensic dna and protein recovery methods |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776342/ https://www.ncbi.nlm.nih.gov/pubmed/31581206 http://dx.doi.org/10.1371/journal.pone.0223170 |
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