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Genetic background and immunological status influence B cell repertoire diversity in mice

The relationship between the immune repertoire and the physiopathological status of individuals is essential to apprehend the genesis and the evolution of numerous pathologies. Nevertheless, the methodological approaches to understand these complex interactions are challenging. We performed a study...

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Autores principales: Chaaya, Nancy, Shahsavarian, Melody A., Maffucci, Irene, Friboulet, Alain, Offmann, Bernard, Léger, Jean-Benoist, Rousseau, Sylvain, Avalle, Bérangère, Padiolleau-Lefèvre, Séverine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776527/
https://www.ncbi.nlm.nih.gov/pubmed/31582818
http://dx.doi.org/10.1038/s41598-019-50714-y
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author Chaaya, Nancy
Shahsavarian, Melody A.
Maffucci, Irene
Friboulet, Alain
Offmann, Bernard
Léger, Jean-Benoist
Rousseau, Sylvain
Avalle, Bérangère
Padiolleau-Lefèvre, Séverine
author_facet Chaaya, Nancy
Shahsavarian, Melody A.
Maffucci, Irene
Friboulet, Alain
Offmann, Bernard
Léger, Jean-Benoist
Rousseau, Sylvain
Avalle, Bérangère
Padiolleau-Lefèvre, Séverine
author_sort Chaaya, Nancy
collection PubMed
description The relationship between the immune repertoire and the physiopathological status of individuals is essential to apprehend the genesis and the evolution of numerous pathologies. Nevertheless, the methodological approaches to understand these complex interactions are challenging. We performed a study evaluating the diversity harbored by different immune repertoires as a function of their physiopathological status. In this study, we base our analysis on a murine scFv library previously described and representing four different immune repertoires: i) healthy and naïve, ii) healthy and immunized, iii) autoimmune prone and naïve, and iv) autoimmune prone and immunized. This library, 2.6 × 10(9) in size, is submitted to high throughput sequencing (Next Generation Sequencing, NGS) in order to analyze the gene subgroups encoding for immunoglobulins. A comparative study of the distribution of immunoglobulin gene subgroups present in the four libraries has revealed shifts in the B cell repertoire originating from differences in genetic background and immunological status of mice.
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spelling pubmed-67765272019-10-09 Genetic background and immunological status influence B cell repertoire diversity in mice Chaaya, Nancy Shahsavarian, Melody A. Maffucci, Irene Friboulet, Alain Offmann, Bernard Léger, Jean-Benoist Rousseau, Sylvain Avalle, Bérangère Padiolleau-Lefèvre, Séverine Sci Rep Article The relationship between the immune repertoire and the physiopathological status of individuals is essential to apprehend the genesis and the evolution of numerous pathologies. Nevertheless, the methodological approaches to understand these complex interactions are challenging. We performed a study evaluating the diversity harbored by different immune repertoires as a function of their physiopathological status. In this study, we base our analysis on a murine scFv library previously described and representing four different immune repertoires: i) healthy and naïve, ii) healthy and immunized, iii) autoimmune prone and naïve, and iv) autoimmune prone and immunized. This library, 2.6 × 10(9) in size, is submitted to high throughput sequencing (Next Generation Sequencing, NGS) in order to analyze the gene subgroups encoding for immunoglobulins. A comparative study of the distribution of immunoglobulin gene subgroups present in the four libraries has revealed shifts in the B cell repertoire originating from differences in genetic background and immunological status of mice. Nature Publishing Group UK 2019-10-03 /pmc/articles/PMC6776527/ /pubmed/31582818 http://dx.doi.org/10.1038/s41598-019-50714-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chaaya, Nancy
Shahsavarian, Melody A.
Maffucci, Irene
Friboulet, Alain
Offmann, Bernard
Léger, Jean-Benoist
Rousseau, Sylvain
Avalle, Bérangère
Padiolleau-Lefèvre, Séverine
Genetic background and immunological status influence B cell repertoire diversity in mice
title Genetic background and immunological status influence B cell repertoire diversity in mice
title_full Genetic background and immunological status influence B cell repertoire diversity in mice
title_fullStr Genetic background and immunological status influence B cell repertoire diversity in mice
title_full_unstemmed Genetic background and immunological status influence B cell repertoire diversity in mice
title_short Genetic background and immunological status influence B cell repertoire diversity in mice
title_sort genetic background and immunological status influence b cell repertoire diversity in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776527/
https://www.ncbi.nlm.nih.gov/pubmed/31582818
http://dx.doi.org/10.1038/s41598-019-50714-y
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