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Changes of Gene Expression in Euglena gracilis Obtained During the 29(th) DLR Parabolic Flight Campaign
Parabolic flight maneuvers of Novespace’s Airbus A310 ZERO-G produce subsequent phases of hypergravity (about 20 s), microgravity (about 22 s) and another 20 s hypergravity on experiments located in the experiment area of the aircraft. The 29(th) DLR parabolic flight campaign consisted of four conse...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776534/ https://www.ncbi.nlm.nih.gov/pubmed/31582787 http://dx.doi.org/10.1038/s41598-019-50611-4 |
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author | Krüger, Julia Richter, Peter Stoltze, Julia Strauch, Sebastian M. Krüger, Marcus Daiker, Viktor Prasad, Binod Sonnewald, Sophia Reid, Stephen Lebert, Michael |
author_facet | Krüger, Julia Richter, Peter Stoltze, Julia Strauch, Sebastian M. Krüger, Marcus Daiker, Viktor Prasad, Binod Sonnewald, Sophia Reid, Stephen Lebert, Michael |
author_sort | Krüger, Julia |
collection | PubMed |
description | Parabolic flight maneuvers of Novespace’s Airbus A310 ZERO-G produce subsequent phases of hypergravity (about 20 s), microgravity (about 22 s) and another 20 s hypergravity on experiments located in the experiment area of the aircraft. The 29(th) DLR parabolic flight campaign consisted of four consecutive flight days with thirty-one parabolas each day. Euglena gracilis cells were fixed with TRIzol during different acceleration conditions at the first and the last parabola of each flight. Samples were collected and analyzed with microarrays for one-color gene expression analysis. The data indicate significant changes in gene expression in E. gracilis within short time. Hierarchical clustering shows that changes induced by the different accelerations yield reproducible effects at independent flight days. Transcription differed between the first and last parabolas indicating adaptation effects in the course of the flight. Different gene groups were found to be affected in different phases of the parabolic flight, among others, genes involved in signal transduction, calcium signaling, transport mechanisms, metabolic pathways, and stress-response as well as membrane and cytoskeletal proteins. In addition, transcripts of other areas, e.g., DNA and protein modification, were altered. The study contributes to the understanding of short-term effects of microgravity and different accelerations on cells at a molecular level. |
format | Online Article Text |
id | pubmed-6776534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67765342019-10-09 Changes of Gene Expression in Euglena gracilis Obtained During the 29(th) DLR Parabolic Flight Campaign Krüger, Julia Richter, Peter Stoltze, Julia Strauch, Sebastian M. Krüger, Marcus Daiker, Viktor Prasad, Binod Sonnewald, Sophia Reid, Stephen Lebert, Michael Sci Rep Article Parabolic flight maneuvers of Novespace’s Airbus A310 ZERO-G produce subsequent phases of hypergravity (about 20 s), microgravity (about 22 s) and another 20 s hypergravity on experiments located in the experiment area of the aircraft. The 29(th) DLR parabolic flight campaign consisted of four consecutive flight days with thirty-one parabolas each day. Euglena gracilis cells were fixed with TRIzol during different acceleration conditions at the first and the last parabola of each flight. Samples were collected and analyzed with microarrays for one-color gene expression analysis. The data indicate significant changes in gene expression in E. gracilis within short time. Hierarchical clustering shows that changes induced by the different accelerations yield reproducible effects at independent flight days. Transcription differed between the first and last parabolas indicating adaptation effects in the course of the flight. Different gene groups were found to be affected in different phases of the parabolic flight, among others, genes involved in signal transduction, calcium signaling, transport mechanisms, metabolic pathways, and stress-response as well as membrane and cytoskeletal proteins. In addition, transcripts of other areas, e.g., DNA and protein modification, were altered. The study contributes to the understanding of short-term effects of microgravity and different accelerations on cells at a molecular level. Nature Publishing Group UK 2019-10-03 /pmc/articles/PMC6776534/ /pubmed/31582787 http://dx.doi.org/10.1038/s41598-019-50611-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Krüger, Julia Richter, Peter Stoltze, Julia Strauch, Sebastian M. Krüger, Marcus Daiker, Viktor Prasad, Binod Sonnewald, Sophia Reid, Stephen Lebert, Michael Changes of Gene Expression in Euglena gracilis Obtained During the 29(th) DLR Parabolic Flight Campaign |
title | Changes of Gene Expression in Euglena gracilis Obtained During the 29(th) DLR Parabolic Flight Campaign |
title_full | Changes of Gene Expression in Euglena gracilis Obtained During the 29(th) DLR Parabolic Flight Campaign |
title_fullStr | Changes of Gene Expression in Euglena gracilis Obtained During the 29(th) DLR Parabolic Flight Campaign |
title_full_unstemmed | Changes of Gene Expression in Euglena gracilis Obtained During the 29(th) DLR Parabolic Flight Campaign |
title_short | Changes of Gene Expression in Euglena gracilis Obtained During the 29(th) DLR Parabolic Flight Campaign |
title_sort | changes of gene expression in euglena gracilis obtained during the 29(th) dlr parabolic flight campaign |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776534/ https://www.ncbi.nlm.nih.gov/pubmed/31582787 http://dx.doi.org/10.1038/s41598-019-50611-4 |
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