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Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D
According to previous research, natural polyamines exert a role in regulating cell committment and differentiation from stemness during skeletal development. In order to assess whether distinct polyamine patterns are associated with different skeletal cell types, primary cultures of stem cells, chon...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776621/ https://www.ncbi.nlm.nih.gov/pubmed/31582764 http://dx.doi.org/10.1038/s41598-019-50543-z |
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author | Minguzzi, Manuela Guidotti, Serena Platano, Daniela D’Adamo, Stefania Cetrullo, Silvia Assirelli, Elisa Santi, Spartaco Mariani, Erminia Trisolino, Giovanni Filardo, Giuseppe Flamigni, Flavio Borzì, Rosa Maria |
author_facet | Minguzzi, Manuela Guidotti, Serena Platano, Daniela D’Adamo, Stefania Cetrullo, Silvia Assirelli, Elisa Santi, Spartaco Mariani, Erminia Trisolino, Giovanni Filardo, Giuseppe Flamigni, Flavio Borzì, Rosa Maria |
author_sort | Minguzzi, Manuela |
collection | PubMed |
description | According to previous research, natural polyamines exert a role in regulating cell committment and differentiation from stemness during skeletal development. In order to assess whether distinct polyamine patterns are associated with different skeletal cell types, primary cultures of stem cells, chondrocytes or osteoblasts were dedicated for HPLC analysis of intracellular polyamines. Spermine (SPM) and Spermidine (SPD) levels were higher in adipose derived stem cells (ASC) compared to mature skeletal cells, i.e. chondrocytes and osteoblasts, confirming the connection of polyamine content with stemness. To establish whether polyamines can protect ASC against oxidative DNA damage in a 3-D differentiation model, the level of γH2AX was measured by western blot, and found to correlate with age and BMI of patients. Addition of either polyamine to ASC was able to hinder DNA damage in the low micromolecular range, with marked reduction of γH2AX level at 10 µM SPM and 5 µM SPD. Molecular analysis of the mechanisms that might underlie the protective effect of polyamine supplementation evidences a possible involvement of autophagy. Altogether, these results support the idea that polyamines are able to manage both stem cell differentiation and cell oxidative damage, and therefore represent appealing tools for regenerative and cell based applications. |
format | Online Article Text |
id | pubmed-6776621 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67766212019-10-09 Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D Minguzzi, Manuela Guidotti, Serena Platano, Daniela D’Adamo, Stefania Cetrullo, Silvia Assirelli, Elisa Santi, Spartaco Mariani, Erminia Trisolino, Giovanni Filardo, Giuseppe Flamigni, Flavio Borzì, Rosa Maria Sci Rep Article According to previous research, natural polyamines exert a role in regulating cell committment and differentiation from stemness during skeletal development. In order to assess whether distinct polyamine patterns are associated with different skeletal cell types, primary cultures of stem cells, chondrocytes or osteoblasts were dedicated for HPLC analysis of intracellular polyamines. Spermine (SPM) and Spermidine (SPD) levels were higher in adipose derived stem cells (ASC) compared to mature skeletal cells, i.e. chondrocytes and osteoblasts, confirming the connection of polyamine content with stemness. To establish whether polyamines can protect ASC against oxidative DNA damage in a 3-D differentiation model, the level of γH2AX was measured by western blot, and found to correlate with age and BMI of patients. Addition of either polyamine to ASC was able to hinder DNA damage in the low micromolecular range, with marked reduction of γH2AX level at 10 µM SPM and 5 µM SPD. Molecular analysis of the mechanisms that might underlie the protective effect of polyamine supplementation evidences a possible involvement of autophagy. Altogether, these results support the idea that polyamines are able to manage both stem cell differentiation and cell oxidative damage, and therefore represent appealing tools for regenerative and cell based applications. Nature Publishing Group UK 2019-10-03 /pmc/articles/PMC6776621/ /pubmed/31582764 http://dx.doi.org/10.1038/s41598-019-50543-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Minguzzi, Manuela Guidotti, Serena Platano, Daniela D’Adamo, Stefania Cetrullo, Silvia Assirelli, Elisa Santi, Spartaco Mariani, Erminia Trisolino, Giovanni Filardo, Giuseppe Flamigni, Flavio Borzì, Rosa Maria Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D |
title | Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D |
title_full | Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D |
title_fullStr | Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D |
title_full_unstemmed | Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D |
title_short | Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D |
title_sort | polyamine supplementation reduces dna damage in adipose stem cells cultured in 3-d |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776621/ https://www.ncbi.nlm.nih.gov/pubmed/31582764 http://dx.doi.org/10.1038/s41598-019-50543-z |
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