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Exposure to atmospheric pollutants is associated with alterations of gut microbiota in spontaneously hypertensive rats

Atmospheric particulate matter with a diameter <2.5 µm (PM2.5) and pollution are worldwide environmental problems and may have negative effects on cardiovascular disease through the lung and gut. The dynamics of intestinal microflora in response to particulate pollutants is unclear. The present s...

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Autores principales: Chen, Dongmei, Xiao, Chunling, Jin, Huanrong, Yang, Biao, Niu, Jiayu, Yan, Siyuan, Sun, Ye, Zhou, Yuan, Wang, Xiangming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777218/
https://www.ncbi.nlm.nih.gov/pubmed/31602224
http://dx.doi.org/10.3892/etm.2019.7934
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author Chen, Dongmei
Xiao, Chunling
Jin, Huanrong
Yang, Biao
Niu, Jiayu
Yan, Siyuan
Sun, Ye
Zhou, Yuan
Wang, Xiangming
author_facet Chen, Dongmei
Xiao, Chunling
Jin, Huanrong
Yang, Biao
Niu, Jiayu
Yan, Siyuan
Sun, Ye
Zhou, Yuan
Wang, Xiangming
author_sort Chen, Dongmei
collection PubMed
description Atmospheric particulate matter with a diameter <2.5 µm (PM2.5) and pollution are worldwide environmental problems and may have negative effects on cardiovascular disease through the lung and gut. The dynamics of intestinal microflora in response to particulate pollutants is unclear. The present study investigated changes in the gut microbiota related to pollutant exposure using spontaneously hypertensive rats (SHR). DNA was extracted from fecal samples. Amplicon Generation and the quality control of PCR products were performed. PCR products was sequenced on an Illumina HiSeq 2500 platform. Data analysis included: operational taxonomic unit (OTU) clustering and species annotation, alpha diversity, beta diversity, principal coordinates analysis (PCoA), and the use of PICRUSt bioinformatics software. The microbial diversity of the SHR rats was inversely associated with exposure to pollutants. In terms of relative abundance, 24 bacterial genera and 2 genera in particular (Actinobacillus and Fusobacterium) significantly declined, and one genus (Treponema) increased. Moreover, pollutant exposure was associated with the accumulation of genes from the gut microbiota that are implicated in cardiovascular diseases. From the long-term exposure experiment, rats appeared to respond to pollutant injury. In conclusion, these results suggest that the effects of atmospheric pollutants on organisms are not limited to the respiratory tract, but also include the gastrointestinal tract. Pollutants are likely to influence the intestinal microbiota and promote the progression of cardiovascular disease.
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spelling pubmed-67772182019-10-10 Exposure to atmospheric pollutants is associated with alterations of gut microbiota in spontaneously hypertensive rats Chen, Dongmei Xiao, Chunling Jin, Huanrong Yang, Biao Niu, Jiayu Yan, Siyuan Sun, Ye Zhou, Yuan Wang, Xiangming Exp Ther Med Articles Atmospheric particulate matter with a diameter <2.5 µm (PM2.5) and pollution are worldwide environmental problems and may have negative effects on cardiovascular disease through the lung and gut. The dynamics of intestinal microflora in response to particulate pollutants is unclear. The present study investigated changes in the gut microbiota related to pollutant exposure using spontaneously hypertensive rats (SHR). DNA was extracted from fecal samples. Amplicon Generation and the quality control of PCR products were performed. PCR products was sequenced on an Illumina HiSeq 2500 platform. Data analysis included: operational taxonomic unit (OTU) clustering and species annotation, alpha diversity, beta diversity, principal coordinates analysis (PCoA), and the use of PICRUSt bioinformatics software. The microbial diversity of the SHR rats was inversely associated with exposure to pollutants. In terms of relative abundance, 24 bacterial genera and 2 genera in particular (Actinobacillus and Fusobacterium) significantly declined, and one genus (Treponema) increased. Moreover, pollutant exposure was associated with the accumulation of genes from the gut microbiota that are implicated in cardiovascular diseases. From the long-term exposure experiment, rats appeared to respond to pollutant injury. In conclusion, these results suggest that the effects of atmospheric pollutants on organisms are not limited to the respiratory tract, but also include the gastrointestinal tract. Pollutants are likely to influence the intestinal microbiota and promote the progression of cardiovascular disease. D.A. Spandidos 2019-11 2019-08-23 /pmc/articles/PMC6777218/ /pubmed/31602224 http://dx.doi.org/10.3892/etm.2019.7934 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Chen, Dongmei
Xiao, Chunling
Jin, Huanrong
Yang, Biao
Niu, Jiayu
Yan, Siyuan
Sun, Ye
Zhou, Yuan
Wang, Xiangming
Exposure to atmospheric pollutants is associated with alterations of gut microbiota in spontaneously hypertensive rats
title Exposure to atmospheric pollutants is associated with alterations of gut microbiota in spontaneously hypertensive rats
title_full Exposure to atmospheric pollutants is associated with alterations of gut microbiota in spontaneously hypertensive rats
title_fullStr Exposure to atmospheric pollutants is associated with alterations of gut microbiota in spontaneously hypertensive rats
title_full_unstemmed Exposure to atmospheric pollutants is associated with alterations of gut microbiota in spontaneously hypertensive rats
title_short Exposure to atmospheric pollutants is associated with alterations of gut microbiota in spontaneously hypertensive rats
title_sort exposure to atmospheric pollutants is associated with alterations of gut microbiota in spontaneously hypertensive rats
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777218/
https://www.ncbi.nlm.nih.gov/pubmed/31602224
http://dx.doi.org/10.3892/etm.2019.7934
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