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The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms
The ESCRT pathway, comprising the in sequence acting ESCRT-0, -I, -II, -III and Vps4 complexes, conducts the abscission of membranes away from the cytosol. Whereas the components of the central ESCRT-III core complex have been thoroughly investigated, the function of the components of the associated...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777356/ https://www.ncbi.nlm.nih.gov/pubmed/31412999 http://dx.doi.org/10.1242/bio.043422 |
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author | Bäumers, Miriam Klose, Sven Brüser, Christian Haag, Carl Hänsch, Sebastian Pannen, Hendrik Weidtkamp-Peters, Stefanie Feldbrügge, Michael Klein, Thomas |
author_facet | Bäumers, Miriam Klose, Sven Brüser, Christian Haag, Carl Hänsch, Sebastian Pannen, Hendrik Weidtkamp-Peters, Stefanie Feldbrügge, Michael Klein, Thomas |
author_sort | Bäumers, Miriam |
collection | PubMed |
description | The ESCRT pathway, comprising the in sequence acting ESCRT-0, -I, -II, -III and Vps4 complexes, conducts the abscission of membranes away from the cytosol. Whereas the components of the central ESCRT-III core complex have been thoroughly investigated, the function of the components of the associated two auxiliary ESCRT sub-complexes are not well-understood in metazoans, especially at the organismal level. We here present the developmental analysis of the Drosophila orthologs of the auxiliary ESCRTs Chmp5 and Ist1, DChmp5 and DIst1, which belong to the two auxiliary sub-complexes. While each single null mutant displayed mild defects in development, the Dist1 Dchmp5 double mutant displayed a severe defect, indicating that the two genes act synergistically, but in separate pathways. Moreover, the presented results indicate that the auxiliary ESCRTs provide robustness against cold during development of diverse poikilothermic organisms, probably by preventing the accumulation of the ESCRT-III core component Shrub on the endosomal membrane. |
format | Online Article Text |
id | pubmed-6777356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-67773562019-10-07 The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms Bäumers, Miriam Klose, Sven Brüser, Christian Haag, Carl Hänsch, Sebastian Pannen, Hendrik Weidtkamp-Peters, Stefanie Feldbrügge, Michael Klein, Thomas Biol Open Research Article The ESCRT pathway, comprising the in sequence acting ESCRT-0, -I, -II, -III and Vps4 complexes, conducts the abscission of membranes away from the cytosol. Whereas the components of the central ESCRT-III core complex have been thoroughly investigated, the function of the components of the associated two auxiliary ESCRT sub-complexes are not well-understood in metazoans, especially at the organismal level. We here present the developmental analysis of the Drosophila orthologs of the auxiliary ESCRTs Chmp5 and Ist1, DChmp5 and DIst1, which belong to the two auxiliary sub-complexes. While each single null mutant displayed mild defects in development, the Dist1 Dchmp5 double mutant displayed a severe defect, indicating that the two genes act synergistically, but in separate pathways. Moreover, the presented results indicate that the auxiliary ESCRTs provide robustness against cold during development of diverse poikilothermic organisms, probably by preventing the accumulation of the ESCRT-III core component Shrub on the endosomal membrane. The Company of Biologists Ltd 2019-08-14 /pmc/articles/PMC6777356/ /pubmed/31412999 http://dx.doi.org/10.1242/bio.043422 Text en © 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Bäumers, Miriam Klose, Sven Brüser, Christian Haag, Carl Hänsch, Sebastian Pannen, Hendrik Weidtkamp-Peters, Stefanie Feldbrügge, Michael Klein, Thomas The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms |
title | The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms |
title_full | The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms |
title_fullStr | The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms |
title_full_unstemmed | The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms |
title_short | The auxiliary ESCRT complexes provide robustness to cold in poikilothermic organisms |
title_sort | auxiliary escrt complexes provide robustness to cold in poikilothermic organisms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777356/ https://www.ncbi.nlm.nih.gov/pubmed/31412999 http://dx.doi.org/10.1242/bio.043422 |
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