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miR-134-5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model
Forkhead box P2 (Foxp2) is a transcription factor involved in vocal learning. However, the number of previous studies that have investigated the role of Foxp2 in early vascular dementia (VD) is limited. The aim of the present study was to determine whether microRNA (miR)-134-5p/Foxp2 contributes to...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777691/ https://www.ncbi.nlm.nih.gov/pubmed/31545395 http://dx.doi.org/10.3892/ijmm.2019.4331 |
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author | Liu, Xin Zhang, Ruilin Wu, Zimei Si, Wenwen Ren, Zhenxing Zhang, Saixia Zhou, Jianhong Chen, Dongfeng |
author_facet | Liu, Xin Zhang, Ruilin Wu, Zimei Si, Wenwen Ren, Zhenxing Zhang, Saixia Zhou, Jianhong Chen, Dongfeng |
author_sort | Liu, Xin |
collection | PubMed |
description | Forkhead box P2 (Foxp2) is a transcription factor involved in vocal learning. However, the number of previous studies that have investigated the role of Foxp2 in early vascular dementia (VD) is limited. The aim of the present study was to determine whether microRNA (miR)-134-5p/Foxp2 contributes to cognitive impairment in a chronic ischemia-induced early VD model. miR-134-5p was found to be significantly increased in the cortex in a rat VD model. Intracerebroventricular injection of miR-134-5p antagomir into VD rats prevented the loss of synaptic proteins and the development of cognitive impairment phenotypes. Histopathological analysis revealed that miR-134-5p aggravated cognitive impairment in VD rats through damage to cortical neurons and loss of synaptic proteins. Bioinformatics analysis predicted that miR-134-5p targets Foxp2 mRNA. Dual luciferase analysis and western blotting supported the prediction that miR-134-5p targets Foxp2. Furthermore, the silencing of Foxp2 significantly inhibited the effect of miR-134-5p on synaptic protein loss. Chromatin immunoprecipitation-quantitative polymerase chain reaction analysis indicated that Foxp2 binds to the synapsin I (Syn1) promoter at -400/-600 bp upstream of the transcription start site. In conclusion, the miR-134-5p/Foxp2/Syn1 axis was found to contribute to cognitive impairment in a chronic ischemia-induced early VD model, which may enable the development of new therapeutic strategies for the prevention and treatment of VD. |
format | Online Article Text |
id | pubmed-6777691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-67776912019-10-09 miR-134-5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model Liu, Xin Zhang, Ruilin Wu, Zimei Si, Wenwen Ren, Zhenxing Zhang, Saixia Zhou, Jianhong Chen, Dongfeng Int J Mol Med Articles Forkhead box P2 (Foxp2) is a transcription factor involved in vocal learning. However, the number of previous studies that have investigated the role of Foxp2 in early vascular dementia (VD) is limited. The aim of the present study was to determine whether microRNA (miR)-134-5p/Foxp2 contributes to cognitive impairment in a chronic ischemia-induced early VD model. miR-134-5p was found to be significantly increased in the cortex in a rat VD model. Intracerebroventricular injection of miR-134-5p antagomir into VD rats prevented the loss of synaptic proteins and the development of cognitive impairment phenotypes. Histopathological analysis revealed that miR-134-5p aggravated cognitive impairment in VD rats through damage to cortical neurons and loss of synaptic proteins. Bioinformatics analysis predicted that miR-134-5p targets Foxp2 mRNA. Dual luciferase analysis and western blotting supported the prediction that miR-134-5p targets Foxp2. Furthermore, the silencing of Foxp2 significantly inhibited the effect of miR-134-5p on synaptic protein loss. Chromatin immunoprecipitation-quantitative polymerase chain reaction analysis indicated that Foxp2 binds to the synapsin I (Syn1) promoter at -400/-600 bp upstream of the transcription start site. In conclusion, the miR-134-5p/Foxp2/Syn1 axis was found to contribute to cognitive impairment in a chronic ischemia-induced early VD model, which may enable the development of new therapeutic strategies for the prevention and treatment of VD. D.A. Spandidos 2019-11 2019-09-05 /pmc/articles/PMC6777691/ /pubmed/31545395 http://dx.doi.org/10.3892/ijmm.2019.4331 Text en Copyright: © Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Xin Zhang, Ruilin Wu, Zimei Si, Wenwen Ren, Zhenxing Zhang, Saixia Zhou, Jianhong Chen, Dongfeng miR-134-5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model |
title | miR-134-5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model |
title_full | miR-134-5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model |
title_fullStr | miR-134-5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model |
title_full_unstemmed | miR-134-5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model |
title_short | miR-134-5p/Foxp2/Syn1 is involved in cognitive impairment in an early vascular dementia rat model |
title_sort | mir-134-5p/foxp2/syn1 is involved in cognitive impairment in an early vascular dementia rat model |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777691/ https://www.ncbi.nlm.nih.gov/pubmed/31545395 http://dx.doi.org/10.3892/ijmm.2019.4331 |
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