Cargando…
Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis
Spleen stromal lines which support in vitro hematopoiesis are investigated for their lineage origin and hematopoietic support function in vivo. Marker expression and gene profiling identify a lineage relationship with mesenchymal stem cells and perivascular reticular cells described recently in bone...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777786/ https://www.ncbi.nlm.nih.gov/pubmed/31584977 http://dx.doi.org/10.1371/journal.pone.0223416 |
_version_ | 1783456667616149504 |
---|---|
author | O’Neill, Helen C. Lim, Hong K. Periasamy, Pravin Kumarappan, Lavanya Tan, Jonathan K. H. O’Neill, Terence J. |
author_facet | O’Neill, Helen C. Lim, Hong K. Periasamy, Pravin Kumarappan, Lavanya Tan, Jonathan K. H. O’Neill, Terence J. |
author_sort | O’Neill, Helen C. |
collection | PubMed |
description | Spleen stromal lines which support in vitro hematopoiesis are investigated for their lineage origin and hematopoietic support function in vivo. Marker expression and gene profiling identify a lineage relationship with mesenchymal stem cells and perivascular reticular cells described recently in bone marrow. Stromal lines commonly express Cxcl12, Pdgfra and Pdgfr typical of bone marrow derived perivascular reticular cells but reflect a unique cell type in terms of other gene and marker expression. Their classification as osteoprogenitors is confirmed through ability to undergo osteogenic, but not adipogenic or chondrogenic differentiation. Some stromal lines were shown to form ectopic niches for HSCs following engraftment under the kidney capsule of NOD/SCID mice. The presence of myeloid cells and a higher representation of a specific dendritic-like cell type over other myeloid cells within grafts was consistent with previous in vitro evidence of hematopoietic support capacity. These studies reinforce the role of perivascular/perisinusoidal reticular cells in hematopoiesis and implicate such cells as niches for hematopoiesis in spleen. |
format | Online Article Text |
id | pubmed-6777786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-67777862019-10-13 Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis O’Neill, Helen C. Lim, Hong K. Periasamy, Pravin Kumarappan, Lavanya Tan, Jonathan K. H. O’Neill, Terence J. PLoS One Research Article Spleen stromal lines which support in vitro hematopoiesis are investigated for their lineage origin and hematopoietic support function in vivo. Marker expression and gene profiling identify a lineage relationship with mesenchymal stem cells and perivascular reticular cells described recently in bone marrow. Stromal lines commonly express Cxcl12, Pdgfra and Pdgfr typical of bone marrow derived perivascular reticular cells but reflect a unique cell type in terms of other gene and marker expression. Their classification as osteoprogenitors is confirmed through ability to undergo osteogenic, but not adipogenic or chondrogenic differentiation. Some stromal lines were shown to form ectopic niches for HSCs following engraftment under the kidney capsule of NOD/SCID mice. The presence of myeloid cells and a higher representation of a specific dendritic-like cell type over other myeloid cells within grafts was consistent with previous in vitro evidence of hematopoietic support capacity. These studies reinforce the role of perivascular/perisinusoidal reticular cells in hematopoiesis and implicate such cells as niches for hematopoiesis in spleen. Public Library of Science 2019-10-04 /pmc/articles/PMC6777786/ /pubmed/31584977 http://dx.doi.org/10.1371/journal.pone.0223416 Text en © 2019 O’Neill et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article O’Neill, Helen C. Lim, Hong K. Periasamy, Pravin Kumarappan, Lavanya Tan, Jonathan K. H. O’Neill, Terence J. Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis |
title | Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis |
title_full | Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis |
title_fullStr | Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis |
title_full_unstemmed | Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis |
title_short | Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis |
title_sort | transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6777786/ https://www.ncbi.nlm.nih.gov/pubmed/31584977 http://dx.doi.org/10.1371/journal.pone.0223416 |
work_keys_str_mv | AT oneillhelenc transplantedspleenstromalcellswithosteogenicpotentialsupportectopicmyelopoiesis AT limhongk transplantedspleenstromalcellswithosteogenicpotentialsupportectopicmyelopoiesis AT periasamypravin transplantedspleenstromalcellswithosteogenicpotentialsupportectopicmyelopoiesis AT kumarappanlavanya transplantedspleenstromalcellswithosteogenicpotentialsupportectopicmyelopoiesis AT tanjonathankh transplantedspleenstromalcellswithosteogenicpotentialsupportectopicmyelopoiesis AT oneillterencej transplantedspleenstromalcellswithosteogenicpotentialsupportectopicmyelopoiesis |