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Differential Modulation of Human Innate Lymphoid Cell (ILC) Subsets by IL-10 and TGF-β

Using multiparameter flow cytometry human innate lymphoid cell (ILC) subsets can be detected in the circulation, in relatively low frequencies. Despite the low frequency of ILCs in circulation, ex vivo experiments have demonstrated that these ILCs release extremely large per cell quantities of signa...

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Autores principales: Bonne-Année, Sandra, Bush, Mabel C., Nutman, Thomas B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778123/
https://www.ncbi.nlm.nih.gov/pubmed/31586075
http://dx.doi.org/10.1038/s41598-019-50308-8
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author Bonne-Année, Sandra
Bush, Mabel C.
Nutman, Thomas B.
author_facet Bonne-Année, Sandra
Bush, Mabel C.
Nutman, Thomas B.
author_sort Bonne-Année, Sandra
collection PubMed
description Using multiparameter flow cytometry human innate lymphoid cell (ILC) subsets can be detected in the circulation, in relatively low frequencies. Despite the low frequency of ILCs in circulation, ex vivo experiments have demonstrated that these ILCs release extremely large per cell quantities of signature ILC cytokines following activation. To determine how activated ILC cytokine production is regulated, ILC subsets were activated in the presence or absence of the immunoregulatory cytokines IL-10 and TGF-β. An examination of circulating ILC subsets revealed surface expression of IL-10Rα and mRNA expression of both IL-10Rα and TGF-βR1 for all ILC subsets. Stimulated ILC1 production of IFN-γ was decreased by TGF-β and not IL-10. Interestingly, ILC2s stimulated in the presence of IL-10 had a marked reduction in cytokine production of IL-5 and IL-13 while TGF-β had no effect on ILC2 cytokine production. Ex vivo activated ILC1 and ILC2 subsets were also found to be a source of the immunoregulatory cytokine IL-10, raising the potential for ILC-mediated regulation of immune cells. These findings demonstrate the differential effects of immunoregulatory cytokines IL-10 and TGF-β on activated ILC1 and ILC2 populations ex vivo.
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spelling pubmed-67781232019-10-09 Differential Modulation of Human Innate Lymphoid Cell (ILC) Subsets by IL-10 and TGF-β Bonne-Année, Sandra Bush, Mabel C. Nutman, Thomas B. Sci Rep Article Using multiparameter flow cytometry human innate lymphoid cell (ILC) subsets can be detected in the circulation, in relatively low frequencies. Despite the low frequency of ILCs in circulation, ex vivo experiments have demonstrated that these ILCs release extremely large per cell quantities of signature ILC cytokines following activation. To determine how activated ILC cytokine production is regulated, ILC subsets were activated in the presence or absence of the immunoregulatory cytokines IL-10 and TGF-β. An examination of circulating ILC subsets revealed surface expression of IL-10Rα and mRNA expression of both IL-10Rα and TGF-βR1 for all ILC subsets. Stimulated ILC1 production of IFN-γ was decreased by TGF-β and not IL-10. Interestingly, ILC2s stimulated in the presence of IL-10 had a marked reduction in cytokine production of IL-5 and IL-13 while TGF-β had no effect on ILC2 cytokine production. Ex vivo activated ILC1 and ILC2 subsets were also found to be a source of the immunoregulatory cytokine IL-10, raising the potential for ILC-mediated regulation of immune cells. These findings demonstrate the differential effects of immunoregulatory cytokines IL-10 and TGF-β on activated ILC1 and ILC2 populations ex vivo. Nature Publishing Group UK 2019-10-04 /pmc/articles/PMC6778123/ /pubmed/31586075 http://dx.doi.org/10.1038/s41598-019-50308-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bonne-Année, Sandra
Bush, Mabel C.
Nutman, Thomas B.
Differential Modulation of Human Innate Lymphoid Cell (ILC) Subsets by IL-10 and TGF-β
title Differential Modulation of Human Innate Lymphoid Cell (ILC) Subsets by IL-10 and TGF-β
title_full Differential Modulation of Human Innate Lymphoid Cell (ILC) Subsets by IL-10 and TGF-β
title_fullStr Differential Modulation of Human Innate Lymphoid Cell (ILC) Subsets by IL-10 and TGF-β
title_full_unstemmed Differential Modulation of Human Innate Lymphoid Cell (ILC) Subsets by IL-10 and TGF-β
title_short Differential Modulation of Human Innate Lymphoid Cell (ILC) Subsets by IL-10 and TGF-β
title_sort differential modulation of human innate lymphoid cell (ilc) subsets by il-10 and tgf-β
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778123/
https://www.ncbi.nlm.nih.gov/pubmed/31586075
http://dx.doi.org/10.1038/s41598-019-50308-8
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