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Variation of (13)C and (15)N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.)
Information on the homogeneity and distribution of (13)carbon ((13)C) and nitrogen ((15)N) labeling in winter wheat (Triticum aestivum L.) is limited. We conducted a dual labeling experiment to evaluate the variability of (13)C and (15)N enrichment in aboveground parts of labeled winter wheat plants...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778429/ https://www.ncbi.nlm.nih.gov/pubmed/31592347 http://dx.doi.org/10.7717/peerj.7738 |
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author | Sun, Zhaoan Wu, Shuxia Zhu, Biao Zhang, Yiwen Bol, Roland Chen, Qing Meng, Fanqiao |
author_facet | Sun, Zhaoan Wu, Shuxia Zhu, Biao Zhang, Yiwen Bol, Roland Chen, Qing Meng, Fanqiao |
author_sort | Sun, Zhaoan |
collection | PubMed |
description | Information on the homogeneity and distribution of (13)carbon ((13)C) and nitrogen ((15)N) labeling in winter wheat (Triticum aestivum L.) is limited. We conducted a dual labeling experiment to evaluate the variability of (13)C and (15)N enrichment in aboveground parts of labeled winter wheat plants. Labeling with (13)C and (15)N was performed on non-nitrogen fertilized (−N) and nitrogen fertilized (+N, 250 kg N ha(−1)) plants at the elongation and grain filling stages. Aboveground parts of wheat were destructively sampled at 28 days after labeling. As winter wheat growth progressed, δ(13)C values of wheat ears increased significantly, whereas those of leaves and stems decreased significantly. At the elongation stage, N addition tended to reduce the aboveground δ(13)C values through dilution of C uptake. At the two stages, upper (newly developed) leaves were more highly enriched with (13)C compared with that of lower (aged) leaves. Variability between individual wheat plants and among pots at the grain filling stage was smaller than that at the elongation stage, especially for the −N treatment. Compared with those of (13)C labeling, differences in (15)N excess between aboveground components (leaves and stems) under (15)N labeling conditions were much smaller. We conclude that non-N fertilization and labeling at the grain filling stage may produce more uniformly (13)C-labeled wheat materials, whereas the materials were more highly (13)C-enriched at the elongation stage, although the δ(13)C values were more variable. The (15)N-enriched straw tissues via urea fertilization were more uniformly labeled at the grain filling stage compared with that at the elongation stage. |
format | Online Article Text |
id | pubmed-6778429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67784292019-10-07 Variation of (13)C and (15)N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.) Sun, Zhaoan Wu, Shuxia Zhu, Biao Zhang, Yiwen Bol, Roland Chen, Qing Meng, Fanqiao PeerJ Agricultural Science Information on the homogeneity and distribution of (13)carbon ((13)C) and nitrogen ((15)N) labeling in winter wheat (Triticum aestivum L.) is limited. We conducted a dual labeling experiment to evaluate the variability of (13)C and (15)N enrichment in aboveground parts of labeled winter wheat plants. Labeling with (13)C and (15)N was performed on non-nitrogen fertilized (−N) and nitrogen fertilized (+N, 250 kg N ha(−1)) plants at the elongation and grain filling stages. Aboveground parts of wheat were destructively sampled at 28 days after labeling. As winter wheat growth progressed, δ(13)C values of wheat ears increased significantly, whereas those of leaves and stems decreased significantly. At the elongation stage, N addition tended to reduce the aboveground δ(13)C values through dilution of C uptake. At the two stages, upper (newly developed) leaves were more highly enriched with (13)C compared with that of lower (aged) leaves. Variability between individual wheat plants and among pots at the grain filling stage was smaller than that at the elongation stage, especially for the −N treatment. Compared with those of (13)C labeling, differences in (15)N excess between aboveground components (leaves and stems) under (15)N labeling conditions were much smaller. We conclude that non-N fertilization and labeling at the grain filling stage may produce more uniformly (13)C-labeled wheat materials, whereas the materials were more highly (13)C-enriched at the elongation stage, although the δ(13)C values were more variable. The (15)N-enriched straw tissues via urea fertilization were more uniformly labeled at the grain filling stage compared with that at the elongation stage. PeerJ Inc. 2019-10-02 /pmc/articles/PMC6778429/ /pubmed/31592347 http://dx.doi.org/10.7717/peerj.7738 Text en ©2019 Sun et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Agricultural Science Sun, Zhaoan Wu, Shuxia Zhu, Biao Zhang, Yiwen Bol, Roland Chen, Qing Meng, Fanqiao Variation of (13)C and (15)N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.) |
title | Variation of (13)C and (15)N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.) |
title_full | Variation of (13)C and (15)N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.) |
title_fullStr | Variation of (13)C and (15)N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.) |
title_full_unstemmed | Variation of (13)C and (15)N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.) |
title_short | Variation of (13)C and (15)N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.) |
title_sort | variation of (13)c and (15)n enrichments in different plant components of labeled winter wheat (triticum aestivum l.) |
topic | Agricultural Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778429/ https://www.ncbi.nlm.nih.gov/pubmed/31592347 http://dx.doi.org/10.7717/peerj.7738 |
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