Cargando…

LncRNA GATA6-AS inhibits cancer cell migration and invasion in gallbladder cancer by downregulating miR-421

BACKGROUND: Long non-coding RNS (lncRNA) GATA6-AS regulates endothelial cell growth, which is involved in many types of human diseases. PURPOSE: Our study was carried out to investigate the involvement of GATA6-AS in gallbladder cancer (GBC). PATIENTS AND METHODS: Sixty-two patients with GBC were re...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Kezhi, Tang, Jianwei, Hou, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778446/
https://www.ncbi.nlm.nih.gov/pubmed/31632058
http://dx.doi.org/10.2147/OTT.S212231
_version_ 1783456766664638464
author Li, Kezhi
Tang, Jianwei
Hou, Yong
author_facet Li, Kezhi
Tang, Jianwei
Hou, Yong
author_sort Li, Kezhi
collection PubMed
description BACKGROUND: Long non-coding RNS (lncRNA) GATA6-AS regulates endothelial cell growth, which is involved in many types of human diseases. PURPOSE: Our study was carried out to investigate the involvement of GATA6-AS in gallbladder cancer (GBC). PATIENTS AND METHODS: Sixty-two patients with GBC were recruited in this study. PCR analysis was performed to determine the expression of GATA6-AS, miR-421 and TIMP-2 in tissues or cell lines. Transwell migration and invasion assay was applied. RESULTS: We found that GATA6-AS was downregulated in tumor tissues than in adjacent healthy tissues of GBC patients, and GATA6-AS expression levels in tumor tissues decreased with the increase of clinical stages. MiR-421 was upregulated in tumor tissues than in adjacent healthy tissues of GBC patients and was inversely correlated with the expression levels of GATA6-AS. MiR-421 overexpression failed to significantly affect GATA6-AS in GBC cells, while GATA6-AS overexpression resulted in inhibited miR-421 expression. GATA6-AS overexpression led to decreased migration and invasion rates of GBC cells. MiR-421 overexpression led to increased migration and invasion rates of GBC. Rescue experiments (co-transfection) showed that miR-421 overexpression led to attenuated effects of GATA6-AS overexpression. CONCLUSION: GATA6-AS may inhibit cancer cell migration and invasion in GBC by downregulating miR-421.
format Online
Article
Text
id pubmed-6778446
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-67784462019-10-18 LncRNA GATA6-AS inhibits cancer cell migration and invasion in gallbladder cancer by downregulating miR-421 Li, Kezhi Tang, Jianwei Hou, Yong Onco Targets Ther Original Research BACKGROUND: Long non-coding RNS (lncRNA) GATA6-AS regulates endothelial cell growth, which is involved in many types of human diseases. PURPOSE: Our study was carried out to investigate the involvement of GATA6-AS in gallbladder cancer (GBC). PATIENTS AND METHODS: Sixty-two patients with GBC were recruited in this study. PCR analysis was performed to determine the expression of GATA6-AS, miR-421 and TIMP-2 in tissues or cell lines. Transwell migration and invasion assay was applied. RESULTS: We found that GATA6-AS was downregulated in tumor tissues than in adjacent healthy tissues of GBC patients, and GATA6-AS expression levels in tumor tissues decreased with the increase of clinical stages. MiR-421 was upregulated in tumor tissues than in adjacent healthy tissues of GBC patients and was inversely correlated with the expression levels of GATA6-AS. MiR-421 overexpression failed to significantly affect GATA6-AS in GBC cells, while GATA6-AS overexpression resulted in inhibited miR-421 expression. GATA6-AS overexpression led to decreased migration and invasion rates of GBC cells. MiR-421 overexpression led to increased migration and invasion rates of GBC. Rescue experiments (co-transfection) showed that miR-421 overexpression led to attenuated effects of GATA6-AS overexpression. CONCLUSION: GATA6-AS may inhibit cancer cell migration and invasion in GBC by downregulating miR-421. Dove 2019-10-01 /pmc/articles/PMC6778446/ /pubmed/31632058 http://dx.doi.org/10.2147/OTT.S212231 Text en © 2019 Li et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Li, Kezhi
Tang, Jianwei
Hou, Yong
LncRNA GATA6-AS inhibits cancer cell migration and invasion in gallbladder cancer by downregulating miR-421
title LncRNA GATA6-AS inhibits cancer cell migration and invasion in gallbladder cancer by downregulating miR-421
title_full LncRNA GATA6-AS inhibits cancer cell migration and invasion in gallbladder cancer by downregulating miR-421
title_fullStr LncRNA GATA6-AS inhibits cancer cell migration and invasion in gallbladder cancer by downregulating miR-421
title_full_unstemmed LncRNA GATA6-AS inhibits cancer cell migration and invasion in gallbladder cancer by downregulating miR-421
title_short LncRNA GATA6-AS inhibits cancer cell migration and invasion in gallbladder cancer by downregulating miR-421
title_sort lncrna gata6-as inhibits cancer cell migration and invasion in gallbladder cancer by downregulating mir-421
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778446/
https://www.ncbi.nlm.nih.gov/pubmed/31632058
http://dx.doi.org/10.2147/OTT.S212231
work_keys_str_mv AT likezhi lncrnagata6asinhibitscancercellmigrationandinvasioningallbladdercancerbydownregulatingmir421
AT tangjianwei lncrnagata6asinhibitscancercellmigrationandinvasioningallbladdercancerbydownregulatingmir421
AT houyong lncrnagata6asinhibitscancercellmigrationandinvasioningallbladdercancerbydownregulatingmir421