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Fine Mapping Using Whole-Genome Sequencing Confirms Anti-Müllerian Hormone as a Major Gene for Sex Determination in Farmed Nile Tilapia (Oreochromis niloticus L.)

Nile tilapia (Oreochromis niloticus) is one of the most cultivated and economically important species in world aquaculture. Intensive production promotes the use of monosex animals, due to an important dimorphism that favors male growth. Currently, the main mechanism to obtain all-male populations i...

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Autores principales: Cáceres, Giovanna, López, María E., Cádiz, María I., Yoshida, Grazyella M., Jedlicki, Ana, Palma-Véjares, Ricardo, Travisany, Dante, Díaz-Domínguez, Diego, Maass, Alejandro, Lhorente, Jean P., Soto, Jose, Salas, Diego, Yáñez, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778786/
https://www.ncbi.nlm.nih.gov/pubmed/31416805
http://dx.doi.org/10.1534/g3.119.400297
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author Cáceres, Giovanna
López, María E.
Cádiz, María I.
Yoshida, Grazyella M.
Jedlicki, Ana
Palma-Véjares, Ricardo
Travisany, Dante
Díaz-Domínguez, Diego
Maass, Alejandro
Lhorente, Jean P.
Soto, Jose
Salas, Diego
Yáñez, José M.
author_facet Cáceres, Giovanna
López, María E.
Cádiz, María I.
Yoshida, Grazyella M.
Jedlicki, Ana
Palma-Véjares, Ricardo
Travisany, Dante
Díaz-Domínguez, Diego
Maass, Alejandro
Lhorente, Jean P.
Soto, Jose
Salas, Diego
Yáñez, José M.
author_sort Cáceres, Giovanna
collection PubMed
description Nile tilapia (Oreochromis niloticus) is one of the most cultivated and economically important species in world aquaculture. Intensive production promotes the use of monosex animals, due to an important dimorphism that favors male growth. Currently, the main mechanism to obtain all-male populations is the use of hormones in feeding during larval and fry phases. Identifying genomic regions associated with sex determination in Nile tilapia is a research topic of great interest. The objective of this study was to identify genomic variants associated with sex determination in three commercial populations of Nile tilapia. Whole-genome sequencing of 326 individuals was performed, and a total of 2.4 million high-quality bi-allelic single nucleotide polymorphisms (SNPs) were identified after quality control. A genome-wide association study (GWAS) was conducted to identify markers associated with the binary sex trait (males = 1; females = 0). A mixed logistic regression GWAS model was fitted and a genome-wide significant signal comprising 36 SNPs, spanning a genomic region of 536 kb in chromosome 23 was identified. Ten out of these 36 genetic variants intercept the anti-Müllerian (Amh) hormone gene. Other significant SNPs were located in the neighboring Amh gene region. This gene has been strongly associated with sex determination in several vertebrate species, playing an essential role in the differentiation of male and female reproductive tissue in early stages of development. This finding provides useful information to better understand the genetic mechanisms underlying sex determination in Nile tilapia.
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spelling pubmed-67787862019-10-07 Fine Mapping Using Whole-Genome Sequencing Confirms Anti-Müllerian Hormone as a Major Gene for Sex Determination in Farmed Nile Tilapia (Oreochromis niloticus L.) Cáceres, Giovanna López, María E. Cádiz, María I. Yoshida, Grazyella M. Jedlicki, Ana Palma-Véjares, Ricardo Travisany, Dante Díaz-Domínguez, Diego Maass, Alejandro Lhorente, Jean P. Soto, Jose Salas, Diego Yáñez, José M. G3 (Bethesda) Genetics of Sex Nile tilapia (Oreochromis niloticus) is one of the most cultivated and economically important species in world aquaculture. Intensive production promotes the use of monosex animals, due to an important dimorphism that favors male growth. Currently, the main mechanism to obtain all-male populations is the use of hormones in feeding during larval and fry phases. Identifying genomic regions associated with sex determination in Nile tilapia is a research topic of great interest. The objective of this study was to identify genomic variants associated with sex determination in three commercial populations of Nile tilapia. Whole-genome sequencing of 326 individuals was performed, and a total of 2.4 million high-quality bi-allelic single nucleotide polymorphisms (SNPs) were identified after quality control. A genome-wide association study (GWAS) was conducted to identify markers associated with the binary sex trait (males = 1; females = 0). A mixed logistic regression GWAS model was fitted and a genome-wide significant signal comprising 36 SNPs, spanning a genomic region of 536 kb in chromosome 23 was identified. Ten out of these 36 genetic variants intercept the anti-Müllerian (Amh) hormone gene. Other significant SNPs were located in the neighboring Amh gene region. This gene has been strongly associated with sex determination in several vertebrate species, playing an essential role in the differentiation of male and female reproductive tissue in early stages of development. This finding provides useful information to better understand the genetic mechanisms underlying sex determination in Nile tilapia. Genetics Society of America 2019-08-15 /pmc/articles/PMC6778786/ /pubmed/31416805 http://dx.doi.org/10.1534/g3.119.400297 Text en Copyright © Cáceres et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genetics of Sex
Cáceres, Giovanna
López, María E.
Cádiz, María I.
Yoshida, Grazyella M.
Jedlicki, Ana
Palma-Véjares, Ricardo
Travisany, Dante
Díaz-Domínguez, Diego
Maass, Alejandro
Lhorente, Jean P.
Soto, Jose
Salas, Diego
Yáñez, José M.
Fine Mapping Using Whole-Genome Sequencing Confirms Anti-Müllerian Hormone as a Major Gene for Sex Determination in Farmed Nile Tilapia (Oreochromis niloticus L.)
title Fine Mapping Using Whole-Genome Sequencing Confirms Anti-Müllerian Hormone as a Major Gene for Sex Determination in Farmed Nile Tilapia (Oreochromis niloticus L.)
title_full Fine Mapping Using Whole-Genome Sequencing Confirms Anti-Müllerian Hormone as a Major Gene for Sex Determination in Farmed Nile Tilapia (Oreochromis niloticus L.)
title_fullStr Fine Mapping Using Whole-Genome Sequencing Confirms Anti-Müllerian Hormone as a Major Gene for Sex Determination in Farmed Nile Tilapia (Oreochromis niloticus L.)
title_full_unstemmed Fine Mapping Using Whole-Genome Sequencing Confirms Anti-Müllerian Hormone as a Major Gene for Sex Determination in Farmed Nile Tilapia (Oreochromis niloticus L.)
title_short Fine Mapping Using Whole-Genome Sequencing Confirms Anti-Müllerian Hormone as a Major Gene for Sex Determination in Farmed Nile Tilapia (Oreochromis niloticus L.)
title_sort fine mapping using whole-genome sequencing confirms anti-müllerian hormone as a major gene for sex determination in farmed nile tilapia (oreochromis niloticus l.)
topic Genetics of Sex
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778786/
https://www.ncbi.nlm.nih.gov/pubmed/31416805
http://dx.doi.org/10.1534/g3.119.400297
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