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Hydrogen inhibits microglial activation and regulates microglial phenotype in a mouse middle cerebral artery occlusion model

Microglia participate in bi-directional control of brain repair after stroke. Previous studies have demonstrated that hydrogen protects brain after ischemia/reperfusion (I/R) by inhibiting inflammation, but the specific mechanism of anti-inflammatory effect of hydrogen is poorly understood. The goal...

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Autores principales: Huang, Jun-Long, Liu, Wen-Wu, Manaenko, Anatol, Sun, Xue-Jun, Mei, Qi-Yong, Hu, Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779010/
https://www.ncbi.nlm.nih.gov/pubmed/31552875
http://dx.doi.org/10.4103/2045-9912.266987
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author Huang, Jun-Long
Liu, Wen-Wu
Manaenko, Anatol
Sun, Xue-Jun
Mei, Qi-Yong
Hu, Qin
author_facet Huang, Jun-Long
Liu, Wen-Wu
Manaenko, Anatol
Sun, Xue-Jun
Mei, Qi-Yong
Hu, Qin
author_sort Huang, Jun-Long
collection PubMed
description Microglia participate in bi-directional control of brain repair after stroke. Previous studies have demonstrated that hydrogen protects brain after ischemia/reperfusion (I/R) by inhibiting inflammation, but the specific mechanism of anti-inflammatory effect of hydrogen is poorly understood. The goal of our study is to investigate whether inhalation of high concentration hydrogen (HCH) is able to attenuate I/R-induced microglia activation. Eighty C57B/L male mice were divided into four groups: sham, I/R, I/R + HCH and I/R + N(2)/O(2) groups. Assessment of animals happened in “blind” matter. I/R was induced by occlusion of middle cerebral artery for one hour). After one hour, filament was withdrawn, which induced reperfusion. Hydrogen treated I/R animals inhaled mix of 66.7% H(2) balanced with O(2) for 90 minutes, starting immediately after initiation of reperfusion. Control animals (N(2)/O(2)) inhaled mix in which hydrogen was replaced with N(2) for the same time (90 minutes). The brain injury, such as brain infarction and development of brain edema, as well as neurobehavioral deficits were determined 23 hours after reperfusion. Effect of HCH on microglia activation in the ischemic penumbra was investigated by immunostaining also 23 hours after reperfusion. mRNA expression of inflammation related genes was detected by PCR. Our results showed that HCH attenuated brain injury and consequently reduced neurological dysfunction after I/R. Furthermore, we demonstrated that HCH directed microglia polarization towards anti-inflammatory M2 polarization. This study indicates hydrogen may exert neuroprotective effects by inhibiting the microglial activation and regulating microglial polarization. This study was conducted in agreement with the Animal Care and Use Committee (IACUC) and Institutional Animal Care guidelines regulation (Shanghai Jiao Tong University, China (approval No. A2015-011) in November 2015.
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spelling pubmed-67790102019-10-15 Hydrogen inhibits microglial activation and regulates microglial phenotype in a mouse middle cerebral artery occlusion model Huang, Jun-Long Liu, Wen-Wu Manaenko, Anatol Sun, Xue-Jun Mei, Qi-Yong Hu, Qin Med Gas Res Research Article Microglia participate in bi-directional control of brain repair after stroke. Previous studies have demonstrated that hydrogen protects brain after ischemia/reperfusion (I/R) by inhibiting inflammation, but the specific mechanism of anti-inflammatory effect of hydrogen is poorly understood. The goal of our study is to investigate whether inhalation of high concentration hydrogen (HCH) is able to attenuate I/R-induced microglia activation. Eighty C57B/L male mice were divided into four groups: sham, I/R, I/R + HCH and I/R + N(2)/O(2) groups. Assessment of animals happened in “blind” matter. I/R was induced by occlusion of middle cerebral artery for one hour). After one hour, filament was withdrawn, which induced reperfusion. Hydrogen treated I/R animals inhaled mix of 66.7% H(2) balanced with O(2) for 90 minutes, starting immediately after initiation of reperfusion. Control animals (N(2)/O(2)) inhaled mix in which hydrogen was replaced with N(2) for the same time (90 minutes). The brain injury, such as brain infarction and development of brain edema, as well as neurobehavioral deficits were determined 23 hours after reperfusion. Effect of HCH on microglia activation in the ischemic penumbra was investigated by immunostaining also 23 hours after reperfusion. mRNA expression of inflammation related genes was detected by PCR. Our results showed that HCH attenuated brain injury and consequently reduced neurological dysfunction after I/R. Furthermore, we demonstrated that HCH directed microglia polarization towards anti-inflammatory M2 polarization. This study indicates hydrogen may exert neuroprotective effects by inhibiting the microglial activation and regulating microglial polarization. This study was conducted in agreement with the Animal Care and Use Committee (IACUC) and Institutional Animal Care guidelines regulation (Shanghai Jiao Tong University, China (approval No. A2015-011) in November 2015. Wolters Kluwer - Medknow 2019-09-23 /pmc/articles/PMC6779010/ /pubmed/31552875 http://dx.doi.org/10.4103/2045-9912.266987 Text en Copyright: © 2019 Medical Gas Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Research Article
Huang, Jun-Long
Liu, Wen-Wu
Manaenko, Anatol
Sun, Xue-Jun
Mei, Qi-Yong
Hu, Qin
Hydrogen inhibits microglial activation and regulates microglial phenotype in a mouse middle cerebral artery occlusion model
title Hydrogen inhibits microglial activation and regulates microglial phenotype in a mouse middle cerebral artery occlusion model
title_full Hydrogen inhibits microglial activation and regulates microglial phenotype in a mouse middle cerebral artery occlusion model
title_fullStr Hydrogen inhibits microglial activation and regulates microglial phenotype in a mouse middle cerebral artery occlusion model
title_full_unstemmed Hydrogen inhibits microglial activation and regulates microglial phenotype in a mouse middle cerebral artery occlusion model
title_short Hydrogen inhibits microglial activation and regulates microglial phenotype in a mouse middle cerebral artery occlusion model
title_sort hydrogen inhibits microglial activation and regulates microglial phenotype in a mouse middle cerebral artery occlusion model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779010/
https://www.ncbi.nlm.nih.gov/pubmed/31552875
http://dx.doi.org/10.4103/2045-9912.266987
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