Cargando…

Probiotic Bacillus Attenuates Oxidative Stress- Induced Intestinal Injury via p38-Mediated Autophagy

Probiotics have been widely used in maintaining intestinal health and one of their benefits is to enhance host antioxidant capacity. However, the involved molecular mechanisms require further investigated. Autophagy is a self-protection process in response to diverse stresses. We hypothesized that p...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Yanping, Wang, Baikui, Xu, Han, Tang, Li, Li, Yali, Gong, Li, Wang, Yang, Li, Weifen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779063/
https://www.ncbi.nlm.nih.gov/pubmed/31632359
http://dx.doi.org/10.3389/fmicb.2019.02185
_version_ 1783456877056622592
author Wu, Yanping
Wang, Baikui
Xu, Han
Tang, Li
Li, Yali
Gong, Li
Wang, Yang
Li, Weifen
author_facet Wu, Yanping
Wang, Baikui
Xu, Han
Tang, Li
Li, Yali
Gong, Li
Wang, Yang
Li, Weifen
author_sort Wu, Yanping
collection PubMed
description Probiotics have been widely used in maintaining intestinal health and one of their benefits is to enhance host antioxidant capacity. However, the involved molecular mechanisms require further investigated. Autophagy is a self-protection process in response to diverse stresses. We hypothesized that probiotics could modulate intestinal autophagy to alleviate oxidative stress. Sprague-Dawley (SD) rats were orally administered Bacillus SC06 or SC08 daily for 24 days and thereafter received an intraperitoneal injection of diquat (DQ) to induce oxidative stress. We found that rats administered Bacillus SC06 showed more significant intestinal tissue repair and antioxidant properties than those administered SC08, which suggests a strain-specific effect of probiotics. Moreover, SC06 alleviated apoptosis by regulating the expression of Bcl2, Bax and cleaved caspase-3. Further investigations revealed that SC06 triggered autophagy, indicated by the upregulation of LC3 and Beclin1 and the degradation of p62 in rat jejunum and IEC-6 cells. Preincubation with autophagy inhibitor 3-methyladenine (3-MA) significantly aggravated reactive oxygen species (ROS) production and apoptotic cell formation. Furthermore, we demonstrated that p38 MAPK (mitogen-activated protein kinase), not AKT (alpha serine/threonine kinase)/mTOR (mammalian target of rapamycin), was involved in SC06-induced autophagy. Taken together, Bacillus SC06 can alleviate oxidative stress-induced disorders and apoptosis via p38-mediated autophagy. The above findings highlight a novel mechanism underlying the beneficial effects of probiotics as functional food and provide a new perspective on the prevention and treatment of oxidative damages.
format Online
Article
Text
id pubmed-6779063
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-67790632019-10-18 Probiotic Bacillus Attenuates Oxidative Stress- Induced Intestinal Injury via p38-Mediated Autophagy Wu, Yanping Wang, Baikui Xu, Han Tang, Li Li, Yali Gong, Li Wang, Yang Li, Weifen Front Microbiol Microbiology Probiotics have been widely used in maintaining intestinal health and one of their benefits is to enhance host antioxidant capacity. However, the involved molecular mechanisms require further investigated. Autophagy is a self-protection process in response to diverse stresses. We hypothesized that probiotics could modulate intestinal autophagy to alleviate oxidative stress. Sprague-Dawley (SD) rats were orally administered Bacillus SC06 or SC08 daily for 24 days and thereafter received an intraperitoneal injection of diquat (DQ) to induce oxidative stress. We found that rats administered Bacillus SC06 showed more significant intestinal tissue repair and antioxidant properties than those administered SC08, which suggests a strain-specific effect of probiotics. Moreover, SC06 alleviated apoptosis by regulating the expression of Bcl2, Bax and cleaved caspase-3. Further investigations revealed that SC06 triggered autophagy, indicated by the upregulation of LC3 and Beclin1 and the degradation of p62 in rat jejunum and IEC-6 cells. Preincubation with autophagy inhibitor 3-methyladenine (3-MA) significantly aggravated reactive oxygen species (ROS) production and apoptotic cell formation. Furthermore, we demonstrated that p38 MAPK (mitogen-activated protein kinase), not AKT (alpha serine/threonine kinase)/mTOR (mammalian target of rapamycin), was involved in SC06-induced autophagy. Taken together, Bacillus SC06 can alleviate oxidative stress-induced disorders and apoptosis via p38-mediated autophagy. The above findings highlight a novel mechanism underlying the beneficial effects of probiotics as functional food and provide a new perspective on the prevention and treatment of oxidative damages. Frontiers Media S.A. 2019-09-30 /pmc/articles/PMC6779063/ /pubmed/31632359 http://dx.doi.org/10.3389/fmicb.2019.02185 Text en Copyright © 2019 Wu, Wang, Xu, Tang, Li, Gong, Wang and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wu, Yanping
Wang, Baikui
Xu, Han
Tang, Li
Li, Yali
Gong, Li
Wang, Yang
Li, Weifen
Probiotic Bacillus Attenuates Oxidative Stress- Induced Intestinal Injury via p38-Mediated Autophagy
title Probiotic Bacillus Attenuates Oxidative Stress- Induced Intestinal Injury via p38-Mediated Autophagy
title_full Probiotic Bacillus Attenuates Oxidative Stress- Induced Intestinal Injury via p38-Mediated Autophagy
title_fullStr Probiotic Bacillus Attenuates Oxidative Stress- Induced Intestinal Injury via p38-Mediated Autophagy
title_full_unstemmed Probiotic Bacillus Attenuates Oxidative Stress- Induced Intestinal Injury via p38-Mediated Autophagy
title_short Probiotic Bacillus Attenuates Oxidative Stress- Induced Intestinal Injury via p38-Mediated Autophagy
title_sort probiotic bacillus attenuates oxidative stress- induced intestinal injury via p38-mediated autophagy
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779063/
https://www.ncbi.nlm.nih.gov/pubmed/31632359
http://dx.doi.org/10.3389/fmicb.2019.02185
work_keys_str_mv AT wuyanping probioticbacillusattenuatesoxidativestressinducedintestinalinjuryviap38mediatedautophagy
AT wangbaikui probioticbacillusattenuatesoxidativestressinducedintestinalinjuryviap38mediatedautophagy
AT xuhan probioticbacillusattenuatesoxidativestressinducedintestinalinjuryviap38mediatedautophagy
AT tangli probioticbacillusattenuatesoxidativestressinducedintestinalinjuryviap38mediatedautophagy
AT liyali probioticbacillusattenuatesoxidativestressinducedintestinalinjuryviap38mediatedautophagy
AT gongli probioticbacillusattenuatesoxidativestressinducedintestinalinjuryviap38mediatedautophagy
AT wangyang probioticbacillusattenuatesoxidativestressinducedintestinalinjuryviap38mediatedautophagy
AT liweifen probioticbacillusattenuatesoxidativestressinducedintestinalinjuryviap38mediatedautophagy