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An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells
The novel ratiometric fluorescent probe HPQRB with an ESIPT effect based on Michael addition for highly sensitive and fast detection of sulfite in living HepG2 cells is reported. HPQRB can be easily synthesized by a two‐step condensation reaction. HPQRB has a large emission shift (Δλ=116 nm), which...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779067/ https://www.ncbi.nlm.nih.gov/pubmed/31608204 http://dx.doi.org/10.1002/open.201900242 |
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author | Liu, Yupeng Ren, Tian‐Bing Cheng, Dan Hou, Jianing Su, Dongdong Yuan, Lin |
author_facet | Liu, Yupeng Ren, Tian‐Bing Cheng, Dan Hou, Jianing Su, Dongdong Yuan, Lin |
author_sort | Liu, Yupeng |
collection | PubMed |
description | The novel ratiometric fluorescent probe HPQRB with an ESIPT effect based on Michael addition for highly sensitive and fast detection of sulfite in living HepG2 cells is reported. HPQRB can be easily synthesized by a two‐step condensation reaction. HPQRB has a large emission shift (Δλ=116 nm), which is beneficial for fluorescence imaging research, and its sulfite‐responsive site is based on a rhodamine‐like structure with the emission peak at 566 nm, which decreases with increasing sulfite concentration. and its HPQ structure always has an ESIPT effect throughout the reaction process, keeping the emission peak at 450 nm as a self‐reference. In particular, HPQRB has high selectivity for sulfite and responds quickly (within 30 s) with a low detection limit (44 nM). Furthermore, HPQRB has been successfully used for fluorescence imaging of sulfite in HepG2 cells, demonstrating the superior ability to detect sulfite under physiological conditions. |
format | Online Article Text |
id | pubmed-6779067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67790672019-10-11 An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells Liu, Yupeng Ren, Tian‐Bing Cheng, Dan Hou, Jianing Su, Dongdong Yuan, Lin ChemistryOpen Full Papers The novel ratiometric fluorescent probe HPQRB with an ESIPT effect based on Michael addition for highly sensitive and fast detection of sulfite in living HepG2 cells is reported. HPQRB can be easily synthesized by a two‐step condensation reaction. HPQRB has a large emission shift (Δλ=116 nm), which is beneficial for fluorescence imaging research, and its sulfite‐responsive site is based on a rhodamine‐like structure with the emission peak at 566 nm, which decreases with increasing sulfite concentration. and its HPQ structure always has an ESIPT effect throughout the reaction process, keeping the emission peak at 450 nm as a self‐reference. In particular, HPQRB has high selectivity for sulfite and responds quickly (within 30 s) with a low detection limit (44 nM). Furthermore, HPQRB has been successfully used for fluorescence imaging of sulfite in HepG2 cells, demonstrating the superior ability to detect sulfite under physiological conditions. John Wiley and Sons Inc. 2019-10-07 /pmc/articles/PMC6779067/ /pubmed/31608204 http://dx.doi.org/10.1002/open.201900242 Text en © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Full Papers Liu, Yupeng Ren, Tian‐Bing Cheng, Dan Hou, Jianing Su, Dongdong Yuan, Lin An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells |
title | An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells |
title_full | An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells |
title_fullStr | An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells |
title_full_unstemmed | An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells |
title_short | An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells |
title_sort | esipt‐based ratiometric fluorescent probe for highly sensitive and rapid detection of sulfite in living cells |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779067/ https://www.ncbi.nlm.nih.gov/pubmed/31608204 http://dx.doi.org/10.1002/open.201900242 |
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