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MiR-92a Family: A Novel Diagnostic Biomarker and Potential Therapeutic Target in Human Cancers

Purpose: This study tried to explore whether members of miR-92a family contribute to early diagnosis and prognosis for human cancers and how they work. Methods: Integrated meta-analysis retrieved from public repositories was employed to assess the clinical roles of the miR-92a family for cancer diag...

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Autores principales: Jiang, Min, Li, Xuelian, Quan, Xiaowei, Li, Xiaoying, Zhou, Baosen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779690/
https://www.ncbi.nlm.nih.gov/pubmed/31632984
http://dx.doi.org/10.3389/fmolb.2019.00098
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author Jiang, Min
Li, Xuelian
Quan, Xiaowei
Li, Xiaoying
Zhou, Baosen
author_facet Jiang, Min
Li, Xuelian
Quan, Xiaowei
Li, Xiaoying
Zhou, Baosen
author_sort Jiang, Min
collection PubMed
description Purpose: This study tried to explore whether members of miR-92a family contribute to early diagnosis and prognosis for human cancers and how they work. Methods: Integrated meta-analysis retrieved from public repositories was employed to assess the clinical roles of the miR-92a family for cancer diagnosis and prognosis. Expression level of miR-92a was detected by the TCGA database and was confirmed by non-small-cell lung cancer (NSCLC) tissues. Targets of miR-92a were predicted using starbase, and validated by dual luciferase assay. Correlation between miR-92a and the target gene was assessed by linkedOmics while expression of the target gene and its role in cancer prognosis were analyzed with UALCAN and Gepia. Results: We recognized the miR-92a family could serve as a potential diagnostic biomarker with a pooled sensitivity of 0.85 [0.81–0.88] and specificity of 0.86 [0.83–0.90]. The overall hazard ratio (HR) was 2.26 [95% CI: 1.70–3.00] for high expression groups compared to low expression groups. Expression of miR-92a was identified to be upregulated in NSCLC, especially in lung squamous cell carcinoma (LUSC). Results from starbase and dual luciferase assay indicated the regulator of G-protein signaling 3 (RGS3) was a direct target of miR-92a. Statistical negative correlation was found for the expression of miR-92a and RGS3. In addition, expression of RGS3 was downregulated in NSCLC and patients with the high expression had a poor prognosis (HR = 1.3) for LUSC patients. However, results were to the contrary for lung adenocarcinoma (HR = 0.7). Conclusion: This study revealed that miR-92a family could be ideal biomarkers for cancer diagnosis and prognosis, which might function through targeting RGS3.
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spelling pubmed-67796902019-10-18 MiR-92a Family: A Novel Diagnostic Biomarker and Potential Therapeutic Target in Human Cancers Jiang, Min Li, Xuelian Quan, Xiaowei Li, Xiaoying Zhou, Baosen Front Mol Biosci Molecular Biosciences Purpose: This study tried to explore whether members of miR-92a family contribute to early diagnosis and prognosis for human cancers and how they work. Methods: Integrated meta-analysis retrieved from public repositories was employed to assess the clinical roles of the miR-92a family for cancer diagnosis and prognosis. Expression level of miR-92a was detected by the TCGA database and was confirmed by non-small-cell lung cancer (NSCLC) tissues. Targets of miR-92a were predicted using starbase, and validated by dual luciferase assay. Correlation between miR-92a and the target gene was assessed by linkedOmics while expression of the target gene and its role in cancer prognosis were analyzed with UALCAN and Gepia. Results: We recognized the miR-92a family could serve as a potential diagnostic biomarker with a pooled sensitivity of 0.85 [0.81–0.88] and specificity of 0.86 [0.83–0.90]. The overall hazard ratio (HR) was 2.26 [95% CI: 1.70–3.00] for high expression groups compared to low expression groups. Expression of miR-92a was identified to be upregulated in NSCLC, especially in lung squamous cell carcinoma (LUSC). Results from starbase and dual luciferase assay indicated the regulator of G-protein signaling 3 (RGS3) was a direct target of miR-92a. Statistical negative correlation was found for the expression of miR-92a and RGS3. In addition, expression of RGS3 was downregulated in NSCLC and patients with the high expression had a poor prognosis (HR = 1.3) for LUSC patients. However, results were to the contrary for lung adenocarcinoma (HR = 0.7). Conclusion: This study revealed that miR-92a family could be ideal biomarkers for cancer diagnosis and prognosis, which might function through targeting RGS3. Frontiers Media S.A. 2019-10-01 /pmc/articles/PMC6779690/ /pubmed/31632984 http://dx.doi.org/10.3389/fmolb.2019.00098 Text en Copyright © 2019 Jiang, Li, Quan, Li and Zhou. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Jiang, Min
Li, Xuelian
Quan, Xiaowei
Li, Xiaoying
Zhou, Baosen
MiR-92a Family: A Novel Diagnostic Biomarker and Potential Therapeutic Target in Human Cancers
title MiR-92a Family: A Novel Diagnostic Biomarker and Potential Therapeutic Target in Human Cancers
title_full MiR-92a Family: A Novel Diagnostic Biomarker and Potential Therapeutic Target in Human Cancers
title_fullStr MiR-92a Family: A Novel Diagnostic Biomarker and Potential Therapeutic Target in Human Cancers
title_full_unstemmed MiR-92a Family: A Novel Diagnostic Biomarker and Potential Therapeutic Target in Human Cancers
title_short MiR-92a Family: A Novel Diagnostic Biomarker and Potential Therapeutic Target in Human Cancers
title_sort mir-92a family: a novel diagnostic biomarker and potential therapeutic target in human cancers
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779690/
https://www.ncbi.nlm.nih.gov/pubmed/31632984
http://dx.doi.org/10.3389/fmolb.2019.00098
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