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Macrophage Migration Inhibitory Factor Triggers Inflammatory Responses During Very Virulent Infectious Bursal Disease Virus Infection
Infectious bursal disease (IBD) is one of the main threats to the poultry industry worldwide. In China, very virulent IBD virus (vvIBDV) is the main prevalent virus strain, causing inflammation, immunosuppression, and high mortality in young chickens. To determine whether this acute inflammation can...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779731/ https://www.ncbi.nlm.nih.gov/pubmed/31632367 http://dx.doi.org/10.3389/fmicb.2019.02225 |
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author | Liu, Aijing Li, Hui Qi, Xiaole Wang, Qi Yang, Bo Wu, Tiantian Yan, Nana Li, Yue Pan, Qing Gao, Yulong Gao, Li Liu, Changjun Zhang, Yanping Cui, Hongyu Li, Kai Wang, Yongqiang Wang, Xiaomei |
author_facet | Liu, Aijing Li, Hui Qi, Xiaole Wang, Qi Yang, Bo Wu, Tiantian Yan, Nana Li, Yue Pan, Qing Gao, Yulong Gao, Li Liu, Changjun Zhang, Yanping Cui, Hongyu Li, Kai Wang, Yongqiang Wang, Xiaomei |
author_sort | Liu, Aijing |
collection | PubMed |
description | Infectious bursal disease (IBD) is one of the main threats to the poultry industry worldwide. In China, very virulent IBD virus (vvIBDV) is the main prevalent virus strain, causing inflammation, immunosuppression, and high mortality in young chickens. To determine whether this acute inflammation can trigger lesions or even death in chickens, it is important to study the mechanism of vvIBDV pathogenicity. Thus, in the current study, we investigated the inflammation response, bursal lesions, and mortality in chickens caused by vvIBDV at different time points postinfection. Results showed an upregulation of proinflammatory cytokines, including interleukin-1β and interleukin-18, and macrophage infiltration in bursa in response to vvIBDV infection. High-throughput proteomic sequencing based on isobaric tags for relative and absolute quantitation showed that chicken macrophage migration inhibitory factor (chMIF) was upregulated uniquely in primary bursal cells infected with vvIBDV compared with infection by nonpathogenic attenuated IBDV. We confirmed that chMIF was upregulated by vvIBDV infection both in vivo and in vitro. Moreover, chMIF was extracellularly secreted by infected DT40 and primary bursal cells. Further experiments revealed that the secreted chMIF could induce migration of peripheral blood mononuclear cells and promote transcription of proinflammatory cytokines in chicken primary macrophages. Notably, these effects of chMIF could be reduced by using an MIF specific inhibitor. Thus, our study elucidates critical molecular determinants underlying vvIBDV-mediated initiation of acute inflammation, which might be pivotal to understand the mechanism of vvIBDV pathogenicity. |
format | Online Article Text |
id | pubmed-6779731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67797312019-10-18 Macrophage Migration Inhibitory Factor Triggers Inflammatory Responses During Very Virulent Infectious Bursal Disease Virus Infection Liu, Aijing Li, Hui Qi, Xiaole Wang, Qi Yang, Bo Wu, Tiantian Yan, Nana Li, Yue Pan, Qing Gao, Yulong Gao, Li Liu, Changjun Zhang, Yanping Cui, Hongyu Li, Kai Wang, Yongqiang Wang, Xiaomei Front Microbiol Microbiology Infectious bursal disease (IBD) is one of the main threats to the poultry industry worldwide. In China, very virulent IBD virus (vvIBDV) is the main prevalent virus strain, causing inflammation, immunosuppression, and high mortality in young chickens. To determine whether this acute inflammation can trigger lesions or even death in chickens, it is important to study the mechanism of vvIBDV pathogenicity. Thus, in the current study, we investigated the inflammation response, bursal lesions, and mortality in chickens caused by vvIBDV at different time points postinfection. Results showed an upregulation of proinflammatory cytokines, including interleukin-1β and interleukin-18, and macrophage infiltration in bursa in response to vvIBDV infection. High-throughput proteomic sequencing based on isobaric tags for relative and absolute quantitation showed that chicken macrophage migration inhibitory factor (chMIF) was upregulated uniquely in primary bursal cells infected with vvIBDV compared with infection by nonpathogenic attenuated IBDV. We confirmed that chMIF was upregulated by vvIBDV infection both in vivo and in vitro. Moreover, chMIF was extracellularly secreted by infected DT40 and primary bursal cells. Further experiments revealed that the secreted chMIF could induce migration of peripheral blood mononuclear cells and promote transcription of proinflammatory cytokines in chicken primary macrophages. Notably, these effects of chMIF could be reduced by using an MIF specific inhibitor. Thus, our study elucidates critical molecular determinants underlying vvIBDV-mediated initiation of acute inflammation, which might be pivotal to understand the mechanism of vvIBDV pathogenicity. Frontiers Media S.A. 2019-10-01 /pmc/articles/PMC6779731/ /pubmed/31632367 http://dx.doi.org/10.3389/fmicb.2019.02225 Text en Copyright © 2019 Liu, Li, Qi, Wang, Yang, Wu, Yan, Li, Pan, Gao, Gao, Liu, Zhang, Cui, Li, Wang and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Liu, Aijing Li, Hui Qi, Xiaole Wang, Qi Yang, Bo Wu, Tiantian Yan, Nana Li, Yue Pan, Qing Gao, Yulong Gao, Li Liu, Changjun Zhang, Yanping Cui, Hongyu Li, Kai Wang, Yongqiang Wang, Xiaomei Macrophage Migration Inhibitory Factor Triggers Inflammatory Responses During Very Virulent Infectious Bursal Disease Virus Infection |
title | Macrophage Migration Inhibitory Factor Triggers Inflammatory Responses During Very Virulent Infectious Bursal Disease Virus Infection |
title_full | Macrophage Migration Inhibitory Factor Triggers Inflammatory Responses During Very Virulent Infectious Bursal Disease Virus Infection |
title_fullStr | Macrophage Migration Inhibitory Factor Triggers Inflammatory Responses During Very Virulent Infectious Bursal Disease Virus Infection |
title_full_unstemmed | Macrophage Migration Inhibitory Factor Triggers Inflammatory Responses During Very Virulent Infectious Bursal Disease Virus Infection |
title_short | Macrophage Migration Inhibitory Factor Triggers Inflammatory Responses During Very Virulent Infectious Bursal Disease Virus Infection |
title_sort | macrophage migration inhibitory factor triggers inflammatory responses during very virulent infectious bursal disease virus infection |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779731/ https://www.ncbi.nlm.nih.gov/pubmed/31632367 http://dx.doi.org/10.3389/fmicb.2019.02225 |
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