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Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology

The mouth is an important niche for bacterial colonization. Previous research used mouth microbiota to predict diseases like colon cancer and inflammatory bowel disease (IBD). It is still unclear how the sampling methodology influences microbial characterization. Our aim was to determine if the samp...

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Autores principales: Hernández-Arriaga, Angélica, Baumann, Anja, Witte, Otto W., Frahm, Christiane, Bergheim, Ina, Camarinha-Silva, Amélia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780121/
https://www.ncbi.nlm.nih.gov/pubmed/31443509
http://dx.doi.org/10.3390/microorganisms7090283
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author Hernández-Arriaga, Angélica
Baumann, Anja
Witte, Otto W.
Frahm, Christiane
Bergheim, Ina
Camarinha-Silva, Amélia
author_facet Hernández-Arriaga, Angélica
Baumann, Anja
Witte, Otto W.
Frahm, Christiane
Bergheim, Ina
Camarinha-Silva, Amélia
author_sort Hernández-Arriaga, Angélica
collection PubMed
description The mouth is an important niche for bacterial colonization. Previous research used mouth microbiota to predict diseases like colon cancer and inflammatory bowel disease (IBD). It is still unclear how the sampling methodology influences microbial characterization. Our aim was to determine if the sampling methods, e.g., cotton swab or tissue biopsy, and the age influence the oral microbial composition of mice. Microbial DNA was extracted using a commercial kit and characterized targeting the 16s rRNA gene from mouth swabs and tissue biopsies from 2 and 15 months old C57BL/6 male mice kept in the same SPF facility. Our results show statistical different microbial community of the different ages, type of sampling, and the two fixed factors age x type of sample (p-value < 0.05). At the genus level, we identified that the genera Actinobacillus, Neisseria, Staphylococcus, and Streptococcus either increase or decrease in abundance depending on sampling and age. Additionally, the abundance of Streptococcus danieliae, Moraxella osloensis, and some unclassified Streptococcus was affected by the sampling method. While swab and tissue biopsies both identified the common colonizers of oral microbiota, cotton swabbing is a low-cost and practical method, validating the use of the swab as the preferred oral sampling approach.
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spelling pubmed-67801212019-10-30 Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology Hernández-Arriaga, Angélica Baumann, Anja Witte, Otto W. Frahm, Christiane Bergheim, Ina Camarinha-Silva, Amélia Microorganisms Brief Report The mouth is an important niche for bacterial colonization. Previous research used mouth microbiota to predict diseases like colon cancer and inflammatory bowel disease (IBD). It is still unclear how the sampling methodology influences microbial characterization. Our aim was to determine if the sampling methods, e.g., cotton swab or tissue biopsy, and the age influence the oral microbial composition of mice. Microbial DNA was extracted using a commercial kit and characterized targeting the 16s rRNA gene from mouth swabs and tissue biopsies from 2 and 15 months old C57BL/6 male mice kept in the same SPF facility. Our results show statistical different microbial community of the different ages, type of sampling, and the two fixed factors age x type of sample (p-value < 0.05). At the genus level, we identified that the genera Actinobacillus, Neisseria, Staphylococcus, and Streptococcus either increase or decrease in abundance depending on sampling and age. Additionally, the abundance of Streptococcus danieliae, Moraxella osloensis, and some unclassified Streptococcus was affected by the sampling method. While swab and tissue biopsies both identified the common colonizers of oral microbiota, cotton swabbing is a low-cost and practical method, validating the use of the swab as the preferred oral sampling approach. MDPI 2019-08-22 /pmc/articles/PMC6780121/ /pubmed/31443509 http://dx.doi.org/10.3390/microorganisms7090283 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Hernández-Arriaga, Angélica
Baumann, Anja
Witte, Otto W.
Frahm, Christiane
Bergheim, Ina
Camarinha-Silva, Amélia
Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology
title Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology
title_full Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology
title_fullStr Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology
title_full_unstemmed Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology
title_short Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology
title_sort changes in oral microbial ecology of c57bl/6 mice at different ages associated with sampling methodology
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780121/
https://www.ncbi.nlm.nih.gov/pubmed/31443509
http://dx.doi.org/10.3390/microorganisms7090283
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