Cargando…

The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin

Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of t...

Descripción completa

Detalles Bibliográficos
Autores principales: Ruiz, Pamela, Balado, Miguel, Fuentes-Monteverde, Juan Carlos, Toranzo, Alicia E., Rodríguez, Jaime, Jiménez, Carlos, Avendaño-Herrera, Ruben, Lemos, Manuel L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780188/
https://www.ncbi.nlm.nih.gov/pubmed/31484388
http://dx.doi.org/10.3390/microorganisms7090313
_version_ 1783457071376629760
author Ruiz, Pamela
Balado, Miguel
Fuentes-Monteverde, Juan Carlos
Toranzo, Alicia E.
Rodríguez, Jaime
Jiménez, Carlos
Avendaño-Herrera, Ruben
Lemos, Manuel L.
author_facet Ruiz, Pamela
Balado, Miguel
Fuentes-Monteverde, Juan Carlos
Toranzo, Alicia E.
Rodríguez, Jaime
Jiménez, Carlos
Avendaño-Herrera, Ruben
Lemos, Manuel L.
author_sort Ruiz, Pamela
collection PubMed
description Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by V. ordalii. Using genetic analysis, identification by peptide mass fingerprinting (PMF) of iron-regulated membrane proteins and chemical identification by LC-HRMS, we were able to clearly demonstrate that V. ordalii produces piscibactin under iron limitation. The synthesis and transport of this siderophore is encoded by a chromosomal gene cluster homologous to another one described in V. anguillarum, which also encodes the synthesis of piscibactin. Using β-galactosidase assays we were able to show that two potential promoters regulated by iron control the transcription of this gene cluster in V. ordalii. Moreover, biosynthetic and transport proteins corresponding to piscibactin synthesis and uptake could be identified in membrane fractions of V. ordalii cells grown under iron limitation. The synthesis of piscibactin was previously reported in other fish pathogens like Photobacterium damselae subsp. piscicida and V. anguillarum, which highlights the importance of this siderophore as a key virulence factor in Vibrionaceae bacteria infecting poikilothermic animals.
format Online
Article
Text
id pubmed-6780188
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67801882019-10-30 The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin Ruiz, Pamela Balado, Miguel Fuentes-Monteverde, Juan Carlos Toranzo, Alicia E. Rodríguez, Jaime Jiménez, Carlos Avendaño-Herrera, Ruben Lemos, Manuel L. Microorganisms Article Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by V. ordalii. Using genetic analysis, identification by peptide mass fingerprinting (PMF) of iron-regulated membrane proteins and chemical identification by LC-HRMS, we were able to clearly demonstrate that V. ordalii produces piscibactin under iron limitation. The synthesis and transport of this siderophore is encoded by a chromosomal gene cluster homologous to another one described in V. anguillarum, which also encodes the synthesis of piscibactin. Using β-galactosidase assays we were able to show that two potential promoters regulated by iron control the transcription of this gene cluster in V. ordalii. Moreover, biosynthetic and transport proteins corresponding to piscibactin synthesis and uptake could be identified in membrane fractions of V. ordalii cells grown under iron limitation. The synthesis of piscibactin was previously reported in other fish pathogens like Photobacterium damselae subsp. piscicida and V. anguillarum, which highlights the importance of this siderophore as a key virulence factor in Vibrionaceae bacteria infecting poikilothermic animals. MDPI 2019-09-03 /pmc/articles/PMC6780188/ /pubmed/31484388 http://dx.doi.org/10.3390/microorganisms7090313 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ruiz, Pamela
Balado, Miguel
Fuentes-Monteverde, Juan Carlos
Toranzo, Alicia E.
Rodríguez, Jaime
Jiménez, Carlos
Avendaño-Herrera, Ruben
Lemos, Manuel L.
The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin
title The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin
title_full The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin
title_fullStr The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin
title_full_unstemmed The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin
title_short The Fish Pathogen Vibrio ordalii Under Iron Deprivation Produces the Siderophore Piscibactin
title_sort fish pathogen vibrio ordalii under iron deprivation produces the siderophore piscibactin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780188/
https://www.ncbi.nlm.nih.gov/pubmed/31484388
http://dx.doi.org/10.3390/microorganisms7090313
work_keys_str_mv AT ruizpamela thefishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT baladomiguel thefishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT fuentesmonteverdejuancarlos thefishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT toranzoaliciae thefishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT rodriguezjaime thefishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT jimenezcarlos thefishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT avendanoherreraruben thefishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT lemosmanuell thefishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT ruizpamela fishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT baladomiguel fishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT fuentesmonteverdejuancarlos fishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT toranzoaliciae fishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT rodriguezjaime fishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT jimenezcarlos fishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT avendanoherreraruben fishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin
AT lemosmanuell fishpathogenvibrioordaliiunderirondeprivationproducesthesiderophorepiscibactin