Cargando…

Scaffold-Free Bioprinter Utilizing Layer-By-Layer Printing of Cellular Spheroids

Free from the limitations posed by exogenous scaffolds or extracellular matrix-based materials, scaffold-free engineered tissues have immense clinical potential. Biomaterials may produce adverse responses, interfere with cell–cell interaction, or affect the extracellular matrix integrity of cells. T...

Descripción completa

Detalles Bibliográficos
Autores principales: LaBarge, Wesley, Morales, Andrés, Pretorius, Daniëlle, Kahn-Krell, Asher M., Kannappan, Ramaswamy, Zhang, Jianyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780220/
https://www.ncbi.nlm.nih.gov/pubmed/31470604
http://dx.doi.org/10.3390/mi10090570
_version_ 1783457079611097088
author LaBarge, Wesley
Morales, Andrés
Pretorius, Daniëlle
Kahn-Krell, Asher M.
Kannappan, Ramaswamy
Zhang, Jianyi
author_facet LaBarge, Wesley
Morales, Andrés
Pretorius, Daniëlle
Kahn-Krell, Asher M.
Kannappan, Ramaswamy
Zhang, Jianyi
author_sort LaBarge, Wesley
collection PubMed
description Free from the limitations posed by exogenous scaffolds or extracellular matrix-based materials, scaffold-free engineered tissues have immense clinical potential. Biomaterials may produce adverse responses, interfere with cell–cell interaction, or affect the extracellular matrix integrity of cells. The scaffold-free Kenzan method can generate complex tissues using spheroids on an array of needles but could be inefficient in terms of time, as it moves and places only a single spheroid at a time. We aimed to design and construct a novel scaffold-free bioprinter that can print an entire layer of spheroids at once, effectively reducing the printing time. The bioprinter was designed using computer-aided design software and constructed from machined, 3D printed, and commercially available parts. The printing efficiency and the operating precision were examined using Zirconia and alginate beads, which mimic spheroids. In less than a minute, the printer could efficiently pick and transfer the beads to the printing surface and assemble them onto the 4 × 4 needles. The average overlap coefficient between layers was measured and found to be 0.997. As a proof of concept using human induced pluripotent stem cell-derived spheroids, we confirmed the ability of the bioprinter to place cellular spheroids onto the needles efficiently to print an entire layer of tissue. This novel layer-by-layer, scaffold-free bioprinter is efficient and precise in operation and can be easily scaled to print large tissues.
format Online
Article
Text
id pubmed-6780220
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67802202019-10-30 Scaffold-Free Bioprinter Utilizing Layer-By-Layer Printing of Cellular Spheroids LaBarge, Wesley Morales, Andrés Pretorius, Daniëlle Kahn-Krell, Asher M. Kannappan, Ramaswamy Zhang, Jianyi Micromachines (Basel) Article Free from the limitations posed by exogenous scaffolds or extracellular matrix-based materials, scaffold-free engineered tissues have immense clinical potential. Biomaterials may produce adverse responses, interfere with cell–cell interaction, or affect the extracellular matrix integrity of cells. The scaffold-free Kenzan method can generate complex tissues using spheroids on an array of needles but could be inefficient in terms of time, as it moves and places only a single spheroid at a time. We aimed to design and construct a novel scaffold-free bioprinter that can print an entire layer of spheroids at once, effectively reducing the printing time. The bioprinter was designed using computer-aided design software and constructed from machined, 3D printed, and commercially available parts. The printing efficiency and the operating precision were examined using Zirconia and alginate beads, which mimic spheroids. In less than a minute, the printer could efficiently pick and transfer the beads to the printing surface and assemble them onto the 4 × 4 needles. The average overlap coefficient between layers was measured and found to be 0.997. As a proof of concept using human induced pluripotent stem cell-derived spheroids, we confirmed the ability of the bioprinter to place cellular spheroids onto the needles efficiently to print an entire layer of tissue. This novel layer-by-layer, scaffold-free bioprinter is efficient and precise in operation and can be easily scaled to print large tissues. MDPI 2019-08-29 /pmc/articles/PMC6780220/ /pubmed/31470604 http://dx.doi.org/10.3390/mi10090570 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
LaBarge, Wesley
Morales, Andrés
Pretorius, Daniëlle
Kahn-Krell, Asher M.
Kannappan, Ramaswamy
Zhang, Jianyi
Scaffold-Free Bioprinter Utilizing Layer-By-Layer Printing of Cellular Spheroids
title Scaffold-Free Bioprinter Utilizing Layer-By-Layer Printing of Cellular Spheroids
title_full Scaffold-Free Bioprinter Utilizing Layer-By-Layer Printing of Cellular Spheroids
title_fullStr Scaffold-Free Bioprinter Utilizing Layer-By-Layer Printing of Cellular Spheroids
title_full_unstemmed Scaffold-Free Bioprinter Utilizing Layer-By-Layer Printing of Cellular Spheroids
title_short Scaffold-Free Bioprinter Utilizing Layer-By-Layer Printing of Cellular Spheroids
title_sort scaffold-free bioprinter utilizing layer-by-layer printing of cellular spheroids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780220/
https://www.ncbi.nlm.nih.gov/pubmed/31470604
http://dx.doi.org/10.3390/mi10090570
work_keys_str_mv AT labargewesley scaffoldfreebioprinterutilizinglayerbylayerprintingofcellularspheroids
AT moralesandres scaffoldfreebioprinterutilizinglayerbylayerprintingofcellularspheroids
AT pretoriusdanielle scaffoldfreebioprinterutilizinglayerbylayerprintingofcellularspheroids
AT kahnkrellasherm scaffoldfreebioprinterutilizinglayerbylayerprintingofcellularspheroids
AT kannappanramaswamy scaffoldfreebioprinterutilizinglayerbylayerprintingofcellularspheroids
AT zhangjianyi scaffoldfreebioprinterutilizinglayerbylayerprintingofcellularspheroids