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The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China

Pennisetum sinese is a good forage grass with high biomass production and crude proteins. However, little is known about the endophytic fungi diversity of P. sinese, which might play an important role in the plant’s growth and biomass production. Here, we used high throughput sequencing of the Inter...

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Autores principales: Deng, Zhen-Shan, Liu, Xiao-Dong, Zhang, Bao-Cheng, Jiao, Shuo, Qi, Xiang-Ying, Sun, Zhi-Hong, He, Xiao-Long, Liu, Yu-Zhen, Li, Jing, Chen, Kai-Kai, Lin, Zhan-Xi, Jiang, Ying-Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780252/
https://www.ncbi.nlm.nih.gov/pubmed/31505744
http://dx.doi.org/10.3390/microorganisms7090332
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author Deng, Zhen-Shan
Liu, Xiao-Dong
Zhang, Bao-Cheng
Jiao, Shuo
Qi, Xiang-Ying
Sun, Zhi-Hong
He, Xiao-Long
Liu, Yu-Zhen
Li, Jing
Chen, Kai-Kai
Lin, Zhan-Xi
Jiang, Ying-Ying
author_facet Deng, Zhen-Shan
Liu, Xiao-Dong
Zhang, Bao-Cheng
Jiao, Shuo
Qi, Xiang-Ying
Sun, Zhi-Hong
He, Xiao-Long
Liu, Yu-Zhen
Li, Jing
Chen, Kai-Kai
Lin, Zhan-Xi
Jiang, Ying-Ying
author_sort Deng, Zhen-Shan
collection PubMed
description Pennisetum sinese is a good forage grass with high biomass production and crude proteins. However, little is known about the endophytic fungi diversity of P. sinese, which might play an important role in the plant’s growth and biomass production. Here, we used high throughput sequencing of the Internal Transcribed Spacer (ITS) sequences based on primers ITS5-1737 and ITS2-2043R to investigate the endophytic fungi diversity of P. sinese roots at the maturity stage, as collected from four provinces (Shaanxi province, SX; Fujian province, FJ; the Xinjiang Uyghur autonomous prefecture, XJ and Inner Mongolia, including sand (NS) and saline-alkali land (NY), China). The ITS sequences were processed using QIIME and R software. A total of 374,875 effective tags were obtained, and 708 operational taxonomic units (OTUs) were yielded with 97% identity in the five samples. Ascomycota and Basidiomycota were the two dominant phyla in the five samples, and the genera Khuskia and Heydenia were the most abundant in the FJ and XJ samples, respectively, while the most abundant tags in the other three samples could not be annotated at the genus level. In addition, our study revealed that the FJ sample possessed the highest OTU numbers (242) and the NS sample had the lowest (86). Moreover, only 22 OTUs were present in all samples simultaneously. The beta diversity analysis suggested a division of two endophytic fungi groups: the FJ sample from the south of China and the other four samples from north or northwest China. Correlation analysis between the environmental factors and endophytic fungi at the class level revealed that Sordariomycetes and Pucciniomycetes had extremely significant positive correlations with the total carbon, annual average precipitation, and annual average temperature, while Leotiomycetes showed an extremely significant negative correlation with quick acting potassium. The results revealed significant differences in the root endophytic fungi diversity of P. sinese in different provinces and might be useful for growth promotion and biomass production in the future.
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spelling pubmed-67802522019-10-30 The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China Deng, Zhen-Shan Liu, Xiao-Dong Zhang, Bao-Cheng Jiao, Shuo Qi, Xiang-Ying Sun, Zhi-Hong He, Xiao-Long Liu, Yu-Zhen Li, Jing Chen, Kai-Kai Lin, Zhan-Xi Jiang, Ying-Ying Microorganisms Article Pennisetum sinese is a good forage grass with high biomass production and crude proteins. However, little is known about the endophytic fungi diversity of P. sinese, which might play an important role in the plant’s growth and biomass production. Here, we used high throughput sequencing of the Internal Transcribed Spacer (ITS) sequences based on primers ITS5-1737 and ITS2-2043R to investigate the endophytic fungi diversity of P. sinese roots at the maturity stage, as collected from four provinces (Shaanxi province, SX; Fujian province, FJ; the Xinjiang Uyghur autonomous prefecture, XJ and Inner Mongolia, including sand (NS) and saline-alkali land (NY), China). The ITS sequences were processed using QIIME and R software. A total of 374,875 effective tags were obtained, and 708 operational taxonomic units (OTUs) were yielded with 97% identity in the five samples. Ascomycota and Basidiomycota were the two dominant phyla in the five samples, and the genera Khuskia and Heydenia were the most abundant in the FJ and XJ samples, respectively, while the most abundant tags in the other three samples could not be annotated at the genus level. In addition, our study revealed that the FJ sample possessed the highest OTU numbers (242) and the NS sample had the lowest (86). Moreover, only 22 OTUs were present in all samples simultaneously. The beta diversity analysis suggested a division of two endophytic fungi groups: the FJ sample from the south of China and the other four samples from north or northwest China. Correlation analysis between the environmental factors and endophytic fungi at the class level revealed that Sordariomycetes and Pucciniomycetes had extremely significant positive correlations with the total carbon, annual average precipitation, and annual average temperature, while Leotiomycetes showed an extremely significant negative correlation with quick acting potassium. The results revealed significant differences in the root endophytic fungi diversity of P. sinese in different provinces and might be useful for growth promotion and biomass production in the future. MDPI 2019-09-09 /pmc/articles/PMC6780252/ /pubmed/31505744 http://dx.doi.org/10.3390/microorganisms7090332 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Deng, Zhen-Shan
Liu, Xiao-Dong
Zhang, Bao-Cheng
Jiao, Shuo
Qi, Xiang-Ying
Sun, Zhi-Hong
He, Xiao-Long
Liu, Yu-Zhen
Li, Jing
Chen, Kai-Kai
Lin, Zhan-Xi
Jiang, Ying-Ying
The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China
title The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China
title_full The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China
title_fullStr The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China
title_full_unstemmed The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China
title_short The Root Endophytic Fungi Community Structure of Pennisetum sinese from Four Representative Provinces in China
title_sort root endophytic fungi community structure of pennisetum sinese from four representative provinces in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780252/
https://www.ncbi.nlm.nih.gov/pubmed/31505744
http://dx.doi.org/10.3390/microorganisms7090332
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