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Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts

Plant species have traditionally been revered for their unparalleled pharmacognostic applications. We outline a non-iterative multi-parallel metabolomic-cum-bioassay-guided methodology toward the functional characterization of ethanol extracts from the Betulaceae family plants (n = 10). We performed...

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Autores principales: Lee, Sunmin, Oh, Dong-Gu, Singh, Digar, Lee, Hye Jin, Kim, Ga Ryun, Lee, Sarah, Lee, Jong Seok, Lee, Choong Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780370/
https://www.ncbi.nlm.nih.gov/pubmed/31527409
http://dx.doi.org/10.3390/metabo9090186
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author Lee, Sunmin
Oh, Dong-Gu
Singh, Digar
Lee, Hye Jin
Kim, Ga Ryun
Lee, Sarah
Lee, Jong Seok
Lee, Choong Hwan
author_facet Lee, Sunmin
Oh, Dong-Gu
Singh, Digar
Lee, Hye Jin
Kim, Ga Ryun
Lee, Sarah
Lee, Jong Seok
Lee, Choong Hwan
author_sort Lee, Sunmin
collection PubMed
description Plant species have traditionally been revered for their unparalleled pharmacognostic applications. We outline a non-iterative multi-parallel metabolomic-cum-bioassay-guided methodology toward the functional characterization of ethanol extracts from the Betulaceae family plants (n = 10). We performed mass spectrometry (MS)-based multivariate analyses and bioassay-guided (ABTS antioxidant activity and cytoprotective effects against H(2)O(2)-induced cell damage) analyses of SPE fractions. A clearly distinct metabolomic pattern coupled with significantly higher bioactivities was observed for 40% methanol SPE eluate. Further, the 40% SPE eluate was subjected to preparative high-performance liquid chromatography (prep-HPLC) analysis, yielding 72 sub-fractions (1 min(−1)), with the highest antioxidant activities observed for the 15 min and 31 min sub-fractions. We simultaneously performed hyphenated-MS-based metabolite characterization of bioactive components for both the 40% methanol SPE fraction and its prep-HPLC sub-fraction (15 min and 31 min). Altogether, 19 candidate metabolites were mainly observed to contribute toward the observed bioactivities. In particular, ethyl gallate was mainly observed to affect the antioxidant activities of SPE and prep-HPLC fractions of Alnus firma extracts. We propose an integrated metabolomic-cum-bioassay-guided approach for the expeditious selection and characterization of discriminant metabolites with desired phenotypes or bioactivities.
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spelling pubmed-67803702019-10-30 Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts Lee, Sunmin Oh, Dong-Gu Singh, Digar Lee, Hye Jin Kim, Ga Ryun Lee, Sarah Lee, Jong Seok Lee, Choong Hwan Metabolites Article Plant species have traditionally been revered for their unparalleled pharmacognostic applications. We outline a non-iterative multi-parallel metabolomic-cum-bioassay-guided methodology toward the functional characterization of ethanol extracts from the Betulaceae family plants (n = 10). We performed mass spectrometry (MS)-based multivariate analyses and bioassay-guided (ABTS antioxidant activity and cytoprotective effects against H(2)O(2)-induced cell damage) analyses of SPE fractions. A clearly distinct metabolomic pattern coupled with significantly higher bioactivities was observed for 40% methanol SPE eluate. Further, the 40% SPE eluate was subjected to preparative high-performance liquid chromatography (prep-HPLC) analysis, yielding 72 sub-fractions (1 min(−1)), with the highest antioxidant activities observed for the 15 min and 31 min sub-fractions. We simultaneously performed hyphenated-MS-based metabolite characterization of bioactive components for both the 40% methanol SPE fraction and its prep-HPLC sub-fraction (15 min and 31 min). Altogether, 19 candidate metabolites were mainly observed to contribute toward the observed bioactivities. In particular, ethyl gallate was mainly observed to affect the antioxidant activities of SPE and prep-HPLC fractions of Alnus firma extracts. We propose an integrated metabolomic-cum-bioassay-guided approach for the expeditious selection and characterization of discriminant metabolites with desired phenotypes or bioactivities. MDPI 2019-09-16 /pmc/articles/PMC6780370/ /pubmed/31527409 http://dx.doi.org/10.3390/metabo9090186 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Sunmin
Oh, Dong-Gu
Singh, Digar
Lee, Hye Jin
Kim, Ga Ryun
Lee, Sarah
Lee, Jong Seok
Lee, Choong Hwan
Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts
title Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts
title_full Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts
title_fullStr Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts
title_full_unstemmed Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts
title_short Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts
title_sort untargeted metabolomics toward systematic characterization of antioxidant compounds in betulaceae family plant extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780370/
https://www.ncbi.nlm.nih.gov/pubmed/31527409
http://dx.doi.org/10.3390/metabo9090186
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