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Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment
Here we report on a non-linear spectroscopic method for visualization of cold atmospheric plasma (CAP)-induced changes in tissue for reaching a new quality level of CAP application in medicine via online monitoring of wound or cancer treatment. A combination of coherent anti-Stokes Raman scattering...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780561/ https://www.ncbi.nlm.nih.gov/pubmed/31454918 http://dx.doi.org/10.3390/mi10090564 |
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author | Meyer, Tobias Bae, Hyeonsoo Hasse, Sybille Winter, Jörn von Woedtke, Thomas Schmitt, Michael Weltmann, Klaus-Dieter Popp, Juergen |
author_facet | Meyer, Tobias Bae, Hyeonsoo Hasse, Sybille Winter, Jörn von Woedtke, Thomas Schmitt, Michael Weltmann, Klaus-Dieter Popp, Juergen |
author_sort | Meyer, Tobias |
collection | PubMed |
description | Here we report on a non-linear spectroscopic method for visualization of cold atmospheric plasma (CAP)-induced changes in tissue for reaching a new quality level of CAP application in medicine via online monitoring of wound or cancer treatment. A combination of coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence lifetime imaging (2P-FLIM) and second harmonic generation (SHG) microscopy has been used for non-invasive and label-free detection of CAP-induced changes on human skin and mucosa samples. By correlation with histochemical staining, the observed local increase in fluorescence could be assigned to melanin. CARS and SHG prove the integrity of the tissue structure, visualize tissue morphology and composition. The influence of plasma effects by variation of plasma parameters e.g., duration of treatment, gas composition and plasma source has been evaluated. Overall quantitative spectroscopic markers could be identified for a direct monitoring of CAP-treated tissue areas, which is very important for translating CAPs into clinical routine. |
format | Online Article Text |
id | pubmed-6780561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67805612019-10-30 Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment Meyer, Tobias Bae, Hyeonsoo Hasse, Sybille Winter, Jörn von Woedtke, Thomas Schmitt, Michael Weltmann, Klaus-Dieter Popp, Juergen Micromachines (Basel) Article Here we report on a non-linear spectroscopic method for visualization of cold atmospheric plasma (CAP)-induced changes in tissue for reaching a new quality level of CAP application in medicine via online monitoring of wound or cancer treatment. A combination of coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence lifetime imaging (2P-FLIM) and second harmonic generation (SHG) microscopy has been used for non-invasive and label-free detection of CAP-induced changes on human skin and mucosa samples. By correlation with histochemical staining, the observed local increase in fluorescence could be assigned to melanin. CARS and SHG prove the integrity of the tissue structure, visualize tissue morphology and composition. The influence of plasma effects by variation of plasma parameters e.g., duration of treatment, gas composition and plasma source has been evaluated. Overall quantitative spectroscopic markers could be identified for a direct monitoring of CAP-treated tissue areas, which is very important for translating CAPs into clinical routine. MDPI 2019-08-26 /pmc/articles/PMC6780561/ /pubmed/31454918 http://dx.doi.org/10.3390/mi10090564 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Meyer, Tobias Bae, Hyeonsoo Hasse, Sybille Winter, Jörn von Woedtke, Thomas Schmitt, Michael Weltmann, Klaus-Dieter Popp, Juergen Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment |
title | Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment |
title_full | Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment |
title_fullStr | Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment |
title_full_unstemmed | Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment |
title_short | Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment |
title_sort | multimodal nonlinear microscopy for therapy monitoring of cold atmospheric plasma treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780561/ https://www.ncbi.nlm.nih.gov/pubmed/31454918 http://dx.doi.org/10.3390/mi10090564 |
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