Cargando…

Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases

Dysbiosis has been described in systemic autoimmune diseases (SADs), including systemic lupus erythematosus (SLE), Sjögren’s syndrome (SjS), and primary anti-phosholipid syndrome (PAPS), however the biological implications of these associations are often elusive. Stool and plasma samples from 114 su...

Descripción completa

Detalles Bibliográficos
Autores principales: Bellocchi, Chiara, Fernández-Ochoa, Álvaro, Montanelli, Gaia, Vigone, Barbara, Santaniello, Alessandro, Quirantes-Piné, Rosa, Borrás-Linares, Isabel, Gerosa, Maria, Artusi, Carolina, Gualtierotti, Roberta, Segura-Carrettero, Antonio, Alarcón-Riquelme, Marta E., Beretta, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780636/
https://www.ncbi.nlm.nih.gov/pubmed/31450824
http://dx.doi.org/10.3390/jcm8091291
_version_ 1783457185056948224
author Bellocchi, Chiara
Fernández-Ochoa, Álvaro
Montanelli, Gaia
Vigone, Barbara
Santaniello, Alessandro
Quirantes-Piné, Rosa
Borrás-Linares, Isabel
Gerosa, Maria
Artusi, Carolina
Gualtierotti, Roberta
Segura-Carrettero, Antonio
Alarcón-Riquelme, Marta E.
Beretta, Lorenzo
author_facet Bellocchi, Chiara
Fernández-Ochoa, Álvaro
Montanelli, Gaia
Vigone, Barbara
Santaniello, Alessandro
Quirantes-Piné, Rosa
Borrás-Linares, Isabel
Gerosa, Maria
Artusi, Carolina
Gualtierotti, Roberta
Segura-Carrettero, Antonio
Alarcón-Riquelme, Marta E.
Beretta, Lorenzo
author_sort Bellocchi, Chiara
collection PubMed
description Dysbiosis has been described in systemic autoimmune diseases (SADs), including systemic lupus erythematosus (SLE), Sjögren’s syndrome (SjS), and primary anti-phosholipid syndrome (PAPS), however the biological implications of these associations are often elusive. Stool and plasma samples from 114 subjects, including in SLE (n = 27), SjS (n = 23), PAPs (n = 11) and undifferentiated connective tissue (UCTD, n = 26) patients, and geographically-matched healthy controls (HCs, n = 27), were collected for microbiome (16s rRNA gene sequencing) and metabolome (high-performance liquid chromatography coupled to mass spectrometry) analysis to identify shared characteristics across diseases. Out of 130 identified microbial genera, a subset of 29 bacteria was able to differentiate study groups (area under receiver operating characteristics (AUROC) = 0.730 ± 0.025). A fair classification was obtained with a subset of 41 metabolic peaks out of 254 (AUROC = 0.748 ± 0.021). In both models, HCs were well separated from SADs, while UCTD largely overlapped with the other diseases. In all of the SADs pro-tolerogenic bacteria were reduced, while pathobiont genera were increased. Metabolic alterations included two clusters comprised of: (a) members of the acylcarnitine family, positively correlating with a Prevotella-enriched cluster and negatively correlating with a butyrate-producing bacteria-enriched cluster; and (b) phospholipids, negatively correlating with butyrate-producing bacteria. These findings demonstrate a strong interaction between intestinal microbiota and metabolic function in patients with SADs.
format Online
Article
Text
id pubmed-6780636
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-67806362019-10-30 Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases Bellocchi, Chiara Fernández-Ochoa, Álvaro Montanelli, Gaia Vigone, Barbara Santaniello, Alessandro Quirantes-Piné, Rosa Borrás-Linares, Isabel Gerosa, Maria Artusi, Carolina Gualtierotti, Roberta Segura-Carrettero, Antonio Alarcón-Riquelme, Marta E. Beretta, Lorenzo J Clin Med Article Dysbiosis has been described in systemic autoimmune diseases (SADs), including systemic lupus erythematosus (SLE), Sjögren’s syndrome (SjS), and primary anti-phosholipid syndrome (PAPS), however the biological implications of these associations are often elusive. Stool and plasma samples from 114 subjects, including in SLE (n = 27), SjS (n = 23), PAPs (n = 11) and undifferentiated connective tissue (UCTD, n = 26) patients, and geographically-matched healthy controls (HCs, n = 27), were collected for microbiome (16s rRNA gene sequencing) and metabolome (high-performance liquid chromatography coupled to mass spectrometry) analysis to identify shared characteristics across diseases. Out of 130 identified microbial genera, a subset of 29 bacteria was able to differentiate study groups (area under receiver operating characteristics (AUROC) = 0.730 ± 0.025). A fair classification was obtained with a subset of 41 metabolic peaks out of 254 (AUROC = 0.748 ± 0.021). In both models, HCs were well separated from SADs, while UCTD largely overlapped with the other diseases. In all of the SADs pro-tolerogenic bacteria were reduced, while pathobiont genera were increased. Metabolic alterations included two clusters comprised of: (a) members of the acylcarnitine family, positively correlating with a Prevotella-enriched cluster and negatively correlating with a butyrate-producing bacteria-enriched cluster; and (b) phospholipids, negatively correlating with butyrate-producing bacteria. These findings demonstrate a strong interaction between intestinal microbiota and metabolic function in patients with SADs. MDPI 2019-08-23 /pmc/articles/PMC6780636/ /pubmed/31450824 http://dx.doi.org/10.3390/jcm8091291 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bellocchi, Chiara
Fernández-Ochoa, Álvaro
Montanelli, Gaia
Vigone, Barbara
Santaniello, Alessandro
Quirantes-Piné, Rosa
Borrás-Linares, Isabel
Gerosa, Maria
Artusi, Carolina
Gualtierotti, Roberta
Segura-Carrettero, Antonio
Alarcón-Riquelme, Marta E.
Beretta, Lorenzo
Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases
title Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases
title_full Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases
title_fullStr Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases
title_full_unstemmed Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases
title_short Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases
title_sort identification of a shared microbiomic and metabolomic profile in systemic autoimmune diseases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780636/
https://www.ncbi.nlm.nih.gov/pubmed/31450824
http://dx.doi.org/10.3390/jcm8091291
work_keys_str_mv AT bellocchichiara identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT fernandezochoaalvaro identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT montanelligaia identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT vigonebarbara identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT santanielloalessandro identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT quirantespinerosa identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT borraslinaresisabel identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT gerosamaria identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT artusicarolina identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT gualtierottiroberta identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT seguracarretteroantonio identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT alarconriquelmemartae identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases
AT berettalorenzo identificationofasharedmicrobiomicandmetabolomicprofileinsystemicautoimmunediseases