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Aptamer-Functionalized Liposomes as a Potential Treatment for Basal Cell Carcinoma

More than one out of every three new cancers is a skin cancer, and the large majority are basal cell carcinomas (BCC). Targeted therapy targets the cancer’s specific genes, proteins, or tissue environment that contributes to cancer growth and survival and blocks the growth as well as the spread of c...

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Autores principales: Cadinoiu, Anca N., Rata, Delia M., Atanase, Leonard I., Daraba, Oana M., Gherghel, Daniela, Vochita, Gabriela, Popa, Marcel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780726/
https://www.ncbi.nlm.nih.gov/pubmed/31540426
http://dx.doi.org/10.3390/polym11091515
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author Cadinoiu, Anca N.
Rata, Delia M.
Atanase, Leonard I.
Daraba, Oana M.
Gherghel, Daniela
Vochita, Gabriela
Popa, Marcel
author_facet Cadinoiu, Anca N.
Rata, Delia M.
Atanase, Leonard I.
Daraba, Oana M.
Gherghel, Daniela
Vochita, Gabriela
Popa, Marcel
author_sort Cadinoiu, Anca N.
collection PubMed
description More than one out of every three new cancers is a skin cancer, and the large majority are basal cell carcinomas (BCC). Targeted therapy targets the cancer’s specific genes, proteins, or tissue environment that contributes to cancer growth and survival and blocks the growth as well as the spread of cancer cells while limiting damage to healthy cells. Therefore, in the present study AS1411 aptamer-functionalized liposomes for the treatment of BCC were obtained and characterized. Aptamer conjugation increased liposome size, suggesting that the presence of an additional hydrophilic molecule on the liposomal surface increased the hydrodynamic diameter. As expected, the negatively charged DNA aptamer reduced the surface potential of the liposomes. Vertical Franz diffusion cells with artificial membranes were used to evaluate the in vitro release of 5-fluorouracil (5-FU). The aptamer moieties increase the stability of the liposomes and act as a supplementary steric barrier leading to a lower cumulative amount of the released 5-FU. The in vitro cell viability, targeting capability and apoptotic effects of liposomes on the human dermal fibroblasts and on the basal cell carcinoma TE 354.T cell lines were also evaluated. The results indicate that the functionalized liposomes are more efficient as nanocarriers than the non-functionalized ones.
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spelling pubmed-67807262019-10-30 Aptamer-Functionalized Liposomes as a Potential Treatment for Basal Cell Carcinoma Cadinoiu, Anca N. Rata, Delia M. Atanase, Leonard I. Daraba, Oana M. Gherghel, Daniela Vochita, Gabriela Popa, Marcel Polymers (Basel) Article More than one out of every three new cancers is a skin cancer, and the large majority are basal cell carcinomas (BCC). Targeted therapy targets the cancer’s specific genes, proteins, or tissue environment that contributes to cancer growth and survival and blocks the growth as well as the spread of cancer cells while limiting damage to healthy cells. Therefore, in the present study AS1411 aptamer-functionalized liposomes for the treatment of BCC were obtained and characterized. Aptamer conjugation increased liposome size, suggesting that the presence of an additional hydrophilic molecule on the liposomal surface increased the hydrodynamic diameter. As expected, the negatively charged DNA aptamer reduced the surface potential of the liposomes. Vertical Franz diffusion cells with artificial membranes were used to evaluate the in vitro release of 5-fluorouracil (5-FU). The aptamer moieties increase the stability of the liposomes and act as a supplementary steric barrier leading to a lower cumulative amount of the released 5-FU. The in vitro cell viability, targeting capability and apoptotic effects of liposomes on the human dermal fibroblasts and on the basal cell carcinoma TE 354.T cell lines were also evaluated. The results indicate that the functionalized liposomes are more efficient as nanocarriers than the non-functionalized ones. MDPI 2019-09-18 /pmc/articles/PMC6780726/ /pubmed/31540426 http://dx.doi.org/10.3390/polym11091515 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cadinoiu, Anca N.
Rata, Delia M.
Atanase, Leonard I.
Daraba, Oana M.
Gherghel, Daniela
Vochita, Gabriela
Popa, Marcel
Aptamer-Functionalized Liposomes as a Potential Treatment for Basal Cell Carcinoma
title Aptamer-Functionalized Liposomes as a Potential Treatment for Basal Cell Carcinoma
title_full Aptamer-Functionalized Liposomes as a Potential Treatment for Basal Cell Carcinoma
title_fullStr Aptamer-Functionalized Liposomes as a Potential Treatment for Basal Cell Carcinoma
title_full_unstemmed Aptamer-Functionalized Liposomes as a Potential Treatment for Basal Cell Carcinoma
title_short Aptamer-Functionalized Liposomes as a Potential Treatment for Basal Cell Carcinoma
title_sort aptamer-functionalized liposomes as a potential treatment for basal cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780726/
https://www.ncbi.nlm.nih.gov/pubmed/31540426
http://dx.doi.org/10.3390/polym11091515
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