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Micro–Macro: Selective Integration of Microfeatures Inside Low-Cost Macromolds for PDMS Microfluidics Fabrication

Microfluidics has become a very promising technology in recent years, due to its great potential to revolutionize life-science solutions. Generic microfabrication processes have been progressively made available to academic laboratories thanks to cost-effective soft-lithography techniques and enable...

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Detalles Bibliográficos
Autores principales: Jiménez-Díaz, Edgar, Cano-Jorge, Mariel, Zamarrón-Hernández, Diego, Cabriales, Lucia, Páez-Larios, Francisco, Cruz-Ramírez, Aarón, Vázquez-Victorio, Genaro, Fiordelisio, Tatiana, Hautefeuille, Mathieu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780727/
https://www.ncbi.nlm.nih.gov/pubmed/31480301
http://dx.doi.org/10.3390/mi10090576
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author Jiménez-Díaz, Edgar
Cano-Jorge, Mariel
Zamarrón-Hernández, Diego
Cabriales, Lucia
Páez-Larios, Francisco
Cruz-Ramírez, Aarón
Vázquez-Victorio, Genaro
Fiordelisio, Tatiana
Hautefeuille, Mathieu
author_facet Jiménez-Díaz, Edgar
Cano-Jorge, Mariel
Zamarrón-Hernández, Diego
Cabriales, Lucia
Páez-Larios, Francisco
Cruz-Ramírez, Aarón
Vázquez-Victorio, Genaro
Fiordelisio, Tatiana
Hautefeuille, Mathieu
author_sort Jiménez-Díaz, Edgar
collection PubMed
description Microfluidics has become a very promising technology in recent years, due to its great potential to revolutionize life-science solutions. Generic microfabrication processes have been progressively made available to academic laboratories thanks to cost-effective soft-lithography techniques and enabled important progress in applications like lab-on-chip platforms using rapid- prototyping. However, micron-sized features are required in most designs, especially in biomimetic cell culture platforms, imposing elevated costs of production associated with lithography and limiting the use of such devices. In most cases, however, only a small portion of the structures require high-resolution and cost may be decreased. In this work, we present a replica-molding method separating the fabrication steps of low (macro) and high (micro) resolutions and then merging the two scales in a single chip. The method consists of fabricating the largest possible area in inexpensive macromolds using simple techniques such as plastics micromilling, laser microfabrication, or even by shrinking printed polystyrene sheets. The microfeatures were made on a separated mold or onto existing macromolds using photolithography or 2-photon lithography. By limiting the expensive area to the essential, the time and cost of fabrication can be reduced. Polydimethylsiloxane (PDMS) microfluidic chips were successfully fabricated from the constructed molds and tested to validate our micro–macro method.
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spelling pubmed-67807272019-10-30 Micro–Macro: Selective Integration of Microfeatures Inside Low-Cost Macromolds for PDMS Microfluidics Fabrication Jiménez-Díaz, Edgar Cano-Jorge, Mariel Zamarrón-Hernández, Diego Cabriales, Lucia Páez-Larios, Francisco Cruz-Ramírez, Aarón Vázquez-Victorio, Genaro Fiordelisio, Tatiana Hautefeuille, Mathieu Micromachines (Basel) Article Microfluidics has become a very promising technology in recent years, due to its great potential to revolutionize life-science solutions. Generic microfabrication processes have been progressively made available to academic laboratories thanks to cost-effective soft-lithography techniques and enabled important progress in applications like lab-on-chip platforms using rapid- prototyping. However, micron-sized features are required in most designs, especially in biomimetic cell culture platforms, imposing elevated costs of production associated with lithography and limiting the use of such devices. In most cases, however, only a small portion of the structures require high-resolution and cost may be decreased. In this work, we present a replica-molding method separating the fabrication steps of low (macro) and high (micro) resolutions and then merging the two scales in a single chip. The method consists of fabricating the largest possible area in inexpensive macromolds using simple techniques such as plastics micromilling, laser microfabrication, or even by shrinking printed polystyrene sheets. The microfeatures were made on a separated mold or onto existing macromolds using photolithography or 2-photon lithography. By limiting the expensive area to the essential, the time and cost of fabrication can be reduced. Polydimethylsiloxane (PDMS) microfluidic chips were successfully fabricated from the constructed molds and tested to validate our micro–macro method. MDPI 2019-08-30 /pmc/articles/PMC6780727/ /pubmed/31480301 http://dx.doi.org/10.3390/mi10090576 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jiménez-Díaz, Edgar
Cano-Jorge, Mariel
Zamarrón-Hernández, Diego
Cabriales, Lucia
Páez-Larios, Francisco
Cruz-Ramírez, Aarón
Vázquez-Victorio, Genaro
Fiordelisio, Tatiana
Hautefeuille, Mathieu
Micro–Macro: Selective Integration of Microfeatures Inside Low-Cost Macromolds for PDMS Microfluidics Fabrication
title Micro–Macro: Selective Integration of Microfeatures Inside Low-Cost Macromolds for PDMS Microfluidics Fabrication
title_full Micro–Macro: Selective Integration of Microfeatures Inside Low-Cost Macromolds for PDMS Microfluidics Fabrication
title_fullStr Micro–Macro: Selective Integration of Microfeatures Inside Low-Cost Macromolds for PDMS Microfluidics Fabrication
title_full_unstemmed Micro–Macro: Selective Integration of Microfeatures Inside Low-Cost Macromolds for PDMS Microfluidics Fabrication
title_short Micro–Macro: Selective Integration of Microfeatures Inside Low-Cost Macromolds for PDMS Microfluidics Fabrication
title_sort micro–macro: selective integration of microfeatures inside low-cost macromolds for pdms microfluidics fabrication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780727/
https://www.ncbi.nlm.nih.gov/pubmed/31480301
http://dx.doi.org/10.3390/mi10090576
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