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Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation

The change in the gut microbiome and microbial metabolites in a patient suffering from severe and enduring anorexia nervosa (AN) and diagnosed with small intestinal bacterial overgrowth syndrome (SIBO) was investigated. Microbial gut dysbiosis is associated with both AN and SIBO, and therefore gut m...

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Autores principales: Prochazkova, Petra, Roubalova, Radka, Dvorak, Jiri, Tlaskalova-Hogenova, Helena, Cermakova, Martina, Tomasova, Petra, Sediva, Blanka, Kuzma, Marek, Bulant, Josef, Bilej, Martin, Hrabak, Pavel, Meisnerova, Eva, Lambertova, Alena, Papezova, Hana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780752/
https://www.ncbi.nlm.nih.gov/pubmed/31510101
http://dx.doi.org/10.3390/microorganisms7090338
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author Prochazkova, Petra
Roubalova, Radka
Dvorak, Jiri
Tlaskalova-Hogenova, Helena
Cermakova, Martina
Tomasova, Petra
Sediva, Blanka
Kuzma, Marek
Bulant, Josef
Bilej, Martin
Hrabak, Pavel
Meisnerova, Eva
Lambertova, Alena
Papezova, Hana
author_facet Prochazkova, Petra
Roubalova, Radka
Dvorak, Jiri
Tlaskalova-Hogenova, Helena
Cermakova, Martina
Tomasova, Petra
Sediva, Blanka
Kuzma, Marek
Bulant, Josef
Bilej, Martin
Hrabak, Pavel
Meisnerova, Eva
Lambertova, Alena
Papezova, Hana
author_sort Prochazkova, Petra
collection PubMed
description The change in the gut microbiome and microbial metabolites in a patient suffering from severe and enduring anorexia nervosa (AN) and diagnosed with small intestinal bacterial overgrowth syndrome (SIBO) was investigated. Microbial gut dysbiosis is associated with both AN and SIBO, and therefore gut microbiome changes by serial fecal microbiota transplantation (FMT) is a possible therapeutic modality. This study assessed the effects of FMT on gut barrier function, microbiota composition, and the levels of bacterial metabolic products. The patient treatment with FMT led to the improvement of gut barrier function, which was altered prior to FMT. Very low bacterial alpha diversity, a lack of beneficial bacteria, together with a great abundance of fungal species were observed in the patient stool sample before FMT. After FMT, both bacterial species richness and gut microbiome evenness increased in the patient, while the fungal alpha diversity decreased. The total short-chain fatty acids (SCFAs) levels (molecules presenting an important source of energy for epithelial gut cells) gradually increased after FMT. Contrarily, one of the most abundant intestinal neurotransmitters, serotonin, tended to decrease throughout the observation period. Overall, gut microbial dysbiosis improvement after FMT was considered. However, there were no signs of patient clinical improvement. The need for an in-depth analysis of the donor´s stool and correct selection pre-FMT is evident.
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spelling pubmed-67807522019-10-30 Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation Prochazkova, Petra Roubalova, Radka Dvorak, Jiri Tlaskalova-Hogenova, Helena Cermakova, Martina Tomasova, Petra Sediva, Blanka Kuzma, Marek Bulant, Josef Bilej, Martin Hrabak, Pavel Meisnerova, Eva Lambertova, Alena Papezova, Hana Microorganisms Case Report The change in the gut microbiome and microbial metabolites in a patient suffering from severe and enduring anorexia nervosa (AN) and diagnosed with small intestinal bacterial overgrowth syndrome (SIBO) was investigated. Microbial gut dysbiosis is associated with both AN and SIBO, and therefore gut microbiome changes by serial fecal microbiota transplantation (FMT) is a possible therapeutic modality. This study assessed the effects of FMT on gut barrier function, microbiota composition, and the levels of bacterial metabolic products. The patient treatment with FMT led to the improvement of gut barrier function, which was altered prior to FMT. Very low bacterial alpha diversity, a lack of beneficial bacteria, together with a great abundance of fungal species were observed in the patient stool sample before FMT. After FMT, both bacterial species richness and gut microbiome evenness increased in the patient, while the fungal alpha diversity decreased. The total short-chain fatty acids (SCFAs) levels (molecules presenting an important source of energy for epithelial gut cells) gradually increased after FMT. Contrarily, one of the most abundant intestinal neurotransmitters, serotonin, tended to decrease throughout the observation period. Overall, gut microbial dysbiosis improvement after FMT was considered. However, there were no signs of patient clinical improvement. The need for an in-depth analysis of the donor´s stool and correct selection pre-FMT is evident. MDPI 2019-09-10 /pmc/articles/PMC6780752/ /pubmed/31510101 http://dx.doi.org/10.3390/microorganisms7090338 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Case Report
Prochazkova, Petra
Roubalova, Radka
Dvorak, Jiri
Tlaskalova-Hogenova, Helena
Cermakova, Martina
Tomasova, Petra
Sediva, Blanka
Kuzma, Marek
Bulant, Josef
Bilej, Martin
Hrabak, Pavel
Meisnerova, Eva
Lambertova, Alena
Papezova, Hana
Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation
title Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation
title_full Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation
title_fullStr Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation
title_full_unstemmed Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation
title_short Microbiota, Microbial Metabolites, and Barrier Function in A Patient with Anorexia Nervosa after Fecal Microbiota Transplantation
title_sort microbiota, microbial metabolites, and barrier function in a patient with anorexia nervosa after fecal microbiota transplantation
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780752/
https://www.ncbi.nlm.nih.gov/pubmed/31510101
http://dx.doi.org/10.3390/microorganisms7090338
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