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Electrospinning of Hyaluronan Using Polymer Coelectrospinning and Intermediate Solvent

In the current study, we present methods of sodium hyaluronate, also denoted as hyaluronan (HA), nanofiber fabrication using a direct-current (DC) electric field. HA was spun in combination with poly(vinyl alcohol) (PVA) and polyethylene oxide (PEO) and as a pure polymer. Nonaggressive solvents were...

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Autores principales: Vítková, Lenka, Musilová, Lenka, Achbergerová, Eva, Minařík, Antonín, Smolka, Petr, Wrzecionko, Erik, Mráček, Aleš
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780821/
https://www.ncbi.nlm.nih.gov/pubmed/31540478
http://dx.doi.org/10.3390/polym11091517
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author Vítková, Lenka
Musilová, Lenka
Achbergerová, Eva
Minařík, Antonín
Smolka, Petr
Wrzecionko, Erik
Mráček, Aleš
author_facet Vítková, Lenka
Musilová, Lenka
Achbergerová, Eva
Minařík, Antonín
Smolka, Petr
Wrzecionko, Erik
Mráček, Aleš
author_sort Vítková, Lenka
collection PubMed
description In the current study, we present methods of sodium hyaluronate, also denoted as hyaluronan (HA), nanofiber fabrication using a direct-current (DC) electric field. HA was spun in combination with poly(vinyl alcohol) (PVA) and polyethylene oxide (PEO) and as a pure polymer. Nonaggressive solvents were used due to the possible use of the fibers in life sciences. The influences of polymer concentration, average molecular weight ([Formula: see text]), viscosity, and solution surface tension were analyzed. HA and PVA were fluorescent-labeled in order to examine the electrospun structures using fluorescence confocal microscopy. In this study, two intermediate solvent mixtures that facilitate HA electrospinning were found. In the case of polymer co-electrospinning, the effect of the surfactant content on the HA/PVA electrospinning process, and the effect of HA [Formula: see text] on HA/PEO nanofiber morphology, were examined, respectively.
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spelling pubmed-67808212019-10-30 Electrospinning of Hyaluronan Using Polymer Coelectrospinning and Intermediate Solvent Vítková, Lenka Musilová, Lenka Achbergerová, Eva Minařík, Antonín Smolka, Petr Wrzecionko, Erik Mráček, Aleš Polymers (Basel) Article In the current study, we present methods of sodium hyaluronate, also denoted as hyaluronan (HA), nanofiber fabrication using a direct-current (DC) electric field. HA was spun in combination with poly(vinyl alcohol) (PVA) and polyethylene oxide (PEO) and as a pure polymer. Nonaggressive solvents were used due to the possible use of the fibers in life sciences. The influences of polymer concentration, average molecular weight ([Formula: see text]), viscosity, and solution surface tension were analyzed. HA and PVA were fluorescent-labeled in order to examine the electrospun structures using fluorescence confocal microscopy. In this study, two intermediate solvent mixtures that facilitate HA electrospinning were found. In the case of polymer co-electrospinning, the effect of the surfactant content on the HA/PVA electrospinning process, and the effect of HA [Formula: see text] on HA/PEO nanofiber morphology, were examined, respectively. MDPI 2019-09-18 /pmc/articles/PMC6780821/ /pubmed/31540478 http://dx.doi.org/10.3390/polym11091517 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vítková, Lenka
Musilová, Lenka
Achbergerová, Eva
Minařík, Antonín
Smolka, Petr
Wrzecionko, Erik
Mráček, Aleš
Electrospinning of Hyaluronan Using Polymer Coelectrospinning and Intermediate Solvent
title Electrospinning of Hyaluronan Using Polymer Coelectrospinning and Intermediate Solvent
title_full Electrospinning of Hyaluronan Using Polymer Coelectrospinning and Intermediate Solvent
title_fullStr Electrospinning of Hyaluronan Using Polymer Coelectrospinning and Intermediate Solvent
title_full_unstemmed Electrospinning of Hyaluronan Using Polymer Coelectrospinning and Intermediate Solvent
title_short Electrospinning of Hyaluronan Using Polymer Coelectrospinning and Intermediate Solvent
title_sort electrospinning of hyaluronan using polymer coelectrospinning and intermediate solvent
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780821/
https://www.ncbi.nlm.nih.gov/pubmed/31540478
http://dx.doi.org/10.3390/polym11091517
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