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Self-Assembled Metal–Organic Biohybrids (MOBs) Using Copper and Silver for Cell Studies

The novel synthesis of metal-containing biohybrids using self-assembly methods at physiological temperatures (37 °C) was compared for copper and silver using the amino acid dimer cystine. Once assembled, the copper containing biohybrid is a stable, high-aspect ratio structure, which we call CuHARS....

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Autores principales: Karekar, Neha, Karan, Anik, Khezerlou, Elnaz, Prajapati, Neela, Pernici, Chelsea D., Murray, Teresa A., DeCoster, Mark A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6781094/
https://www.ncbi.nlm.nih.gov/pubmed/31500351
http://dx.doi.org/10.3390/nano9091282
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author Karekar, Neha
Karan, Anik
Khezerlou, Elnaz
Prajapati, Neela
Pernici, Chelsea D.
Murray, Teresa A.
DeCoster, Mark A.
author_facet Karekar, Neha
Karan, Anik
Khezerlou, Elnaz
Prajapati, Neela
Pernici, Chelsea D.
Murray, Teresa A.
DeCoster, Mark A.
author_sort Karekar, Neha
collection PubMed
description The novel synthesis of metal-containing biohybrids using self-assembly methods at physiological temperatures (37 °C) was compared for copper and silver using the amino acid dimer cystine. Once assembled, the copper containing biohybrid is a stable, high-aspect ratio structure, which we call CuHARS. Using the same synthesis conditions, but replacing copper with silver, we have synthesized cystine-capped silver nanoparticles (AgCysNPs), which are shown here to form stable colloid solutions in contrast to the CuHARS, which settle out from a 1 mg/mL solution in 90 min. Both the copper and silver biohybrids, as synthesized, demonstrate very low agglomeration which we have applied for the purpose of applications with cell culture methods, namely, for testing as anti-cancer compounds. AgCysNPs (1000 ng/mL) demonstrated significant toxicity (only 6.8% viability) to glioma and neuroblastoma cells in vitro, with concentrations as low as 20 ng/mL causing some toxicity. In contrast, CuHARS required at least 5 μg/mL. For comparative purposes, silver sulfate at 100 ng/mL decreased viability by 52% and copper sulfate at 100 ng/mL only by 19.5% on glioma cells. Using these methods, the novel materials were tested here as metal–organic biohybrids (MOBs), and it is anticipated that the functionalization and dynamics of MOBs may result in building a foundation of new materials for cellular applications, including cell engineering of both normal and diseased cells and tissue constructs.
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spelling pubmed-67810942019-10-30 Self-Assembled Metal–Organic Biohybrids (MOBs) Using Copper and Silver for Cell Studies Karekar, Neha Karan, Anik Khezerlou, Elnaz Prajapati, Neela Pernici, Chelsea D. Murray, Teresa A. DeCoster, Mark A. Nanomaterials (Basel) Article The novel synthesis of metal-containing biohybrids using self-assembly methods at physiological temperatures (37 °C) was compared for copper and silver using the amino acid dimer cystine. Once assembled, the copper containing biohybrid is a stable, high-aspect ratio structure, which we call CuHARS. Using the same synthesis conditions, but replacing copper with silver, we have synthesized cystine-capped silver nanoparticles (AgCysNPs), which are shown here to form stable colloid solutions in contrast to the CuHARS, which settle out from a 1 mg/mL solution in 90 min. Both the copper and silver biohybrids, as synthesized, demonstrate very low agglomeration which we have applied for the purpose of applications with cell culture methods, namely, for testing as anti-cancer compounds. AgCysNPs (1000 ng/mL) demonstrated significant toxicity (only 6.8% viability) to glioma and neuroblastoma cells in vitro, with concentrations as low as 20 ng/mL causing some toxicity. In contrast, CuHARS required at least 5 μg/mL. For comparative purposes, silver sulfate at 100 ng/mL decreased viability by 52% and copper sulfate at 100 ng/mL only by 19.5% on glioma cells. Using these methods, the novel materials were tested here as metal–organic biohybrids (MOBs), and it is anticipated that the functionalization and dynamics of MOBs may result in building a foundation of new materials for cellular applications, including cell engineering of both normal and diseased cells and tissue constructs. MDPI 2019-09-08 /pmc/articles/PMC6781094/ /pubmed/31500351 http://dx.doi.org/10.3390/nano9091282 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Karekar, Neha
Karan, Anik
Khezerlou, Elnaz
Prajapati, Neela
Pernici, Chelsea D.
Murray, Teresa A.
DeCoster, Mark A.
Self-Assembled Metal–Organic Biohybrids (MOBs) Using Copper and Silver for Cell Studies
title Self-Assembled Metal–Organic Biohybrids (MOBs) Using Copper and Silver for Cell Studies
title_full Self-Assembled Metal–Organic Biohybrids (MOBs) Using Copper and Silver for Cell Studies
title_fullStr Self-Assembled Metal–Organic Biohybrids (MOBs) Using Copper and Silver for Cell Studies
title_full_unstemmed Self-Assembled Metal–Organic Biohybrids (MOBs) Using Copper and Silver for Cell Studies
title_short Self-Assembled Metal–Organic Biohybrids (MOBs) Using Copper and Silver for Cell Studies
title_sort self-assembled metal–organic biohybrids (mobs) using copper and silver for cell studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6781094/
https://www.ncbi.nlm.nih.gov/pubmed/31500351
http://dx.doi.org/10.3390/nano9091282
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